FastQCFastQC Report
Thu 28 Sept 2023
SRR3199177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14171244
Total Bases722.7 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC1880021.326644294601095No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA1679741.1853158410087357No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT1627141.148198422100417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC1309230.923863847097686Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT1303910.9201097659457419No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC1194860.843158158874408No Hit
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC869660.6136793636465507Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA752730.5311672002824875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTC743510.5246610671582538Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG701160.494776605356594Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC663110.46792645726797166Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT653020.46080640485761165Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT635850.44869031963601785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG625070.44108336572286805TruSeq Adapter, Index 11 (100% over 49bp)
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACG603130.4256013092428583No Hit
AACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTCC570360.40247701613210524Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACT535560.3779202446870578Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACAC527840.37247259309062775No Hit
CGTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCC523190.3691913003544361Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCT520670.36741305138772573Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACT519940.3668979237108612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG511580.3609986533292349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTC481570.33982196622964084No Hit
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC459890.324523379881117No Hit
GTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTCC447990.31612609309387374Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGT410660.2897840161385973No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTC376730.265841163979676No Hit
CGTGCAAAGTCGTACCCATATCCGCAGATCGGAAGAGCACACGTCTGAAC366280.25846707600264307Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAAC357390.25219380881452613Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC356620.25165045496358684No Hit
TGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAA348980.24625925571530627Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAAC326150.23014916686213294Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAAGATCGGAAGAGCACACGT322550.22760881119540388No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG317930.22434868808976827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTGA311590.21987483949891767Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCAC311380.21972665208502512No Hit
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACG302760.21364391157191281No Hit
CCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGT292750.20658031150970232No Hit
GTGTGAGTCCCATCTGACGAGCGAGATCGGAAGAGCACACGTCTGAACTC290320.20486557143466022Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACT287140.20262159059571622Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTAACGGTTAGCACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG284890.20103386830401057Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGTAATGGCTATCACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAG282630.19943908946878625Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACG274790.19390675935013188No Hit
CTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAACT274750.1938785331760571Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACACGTCTG255580.1803511392507249Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTG248420.17529865409134157Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCCA236950.1672047986754021Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA226960.16015531170022898Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC221460.15627421276494852Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGCAAAGTCGTACCCATATCCGCAGAGATCGGAAGAGCACACGTCTGA218750.15436188947138305Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGA216300.15263303630930355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC209270.1476722862156632No Hit
TAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGA206510.14572468020450427Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT206410.14565411476931736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACTC205900.14529423104986408Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT204920.14460268978503227No Hit
TACGAAACCTAGAAGGCAGAGTGAGATCGGAAGAGCACACGTCTGAACTC198190.1398536359969527Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGAAC197100.1390844727534153Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGA192290.13569027532092456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACATGGGTCAGTCGATCCTAAGCTAGATCGGAAGAGCACACGTCT191390.1350551864042423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAAC188870.13327693743753194Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGA187870.13257128308566277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTG187180.13208438158287306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGGGTCAGTCGATCCTAAGCTATAGGAGATCGGAAGAGCACACGTCTGA187070.1320067596041674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACAC182140.1285278836494524No Hit
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG179350.12655910800773734Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGAGTCCCATCTGACGAGCAGATCGGAAGAGCACACGTCTGAACTCC177000.12490082028084477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACTC175400.1237717733178541Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTG175290.1236941513391485Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGAGATCGGAAGAGCACACGTCT173740.12260038709375126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAGTGGAGAAGGTTTCCATGTGAACAGATCGGAAGAGCACACGTCTGA173060.1221205421344802Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGT172150.12147839667427927No Hit
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA169630.11970014770756893Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC168940.1192132462047792Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACG168760.11908622842144274No Hit
GGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCTGAACTCCA167230.1180065772630829Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGC161850.11421015685002672No Hit
ATGGGTCAGTCGATCCTAAGCTATAGGTTAAAAGATCGGAAGAGCACACG161330.11384321658705475No Hit
ATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGAA158090.1115568964869986Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTG156630.1105266411332696Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC154300.10888246649341442Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAACT154020.10868488327489105Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGCAAAGTCGTACCCATATCCGCAGCAGATCGGAAGAGCACACGTCTG153050.10800039855357794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCAGATCGGAAGAGCACACGTCTGAACT152770.10780281533505455Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTCCGATCACTGAAGTCAAACTACGACGAGATCGGAAGAGCACACGTCT151740.10707599135262931Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAACT151430.10685723850354986Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCT151360.10680784269891902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGGTCAGTCGATCCTAAGCTATAGAGATCGGAAGAGCACACGTCTGAA151290.10675844689428818Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAA149550.10553060832203581Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAGATCGGAAGAGCACACGTCT147390.10400639492199838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGAACTCCAG147350.10397816874792361Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTG145540.1027009343710404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG145010.10232693756454973Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC144290.10181886643120391Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTACAATGAGTCGATCAACGTGAGATCGGAAGAGCACACGTCTGAACTC142580.10061219748950763Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCAAAGTCGTACCCATATCCGCAGATCGGAAGAGCACACGTCTGAACT142440.10051340588024595Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTTAATATTCCCGCACTTTGTGACAGATCGGAAGAGCACACGTCTGAAC142210.10035110537931603Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGACCGGTTGGGAATACCTCGTGCAGATCGGAAGAGCACACGTCTGAA141790.10005473055153097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)