FastQCFastQC Report
Thu 28 Sept 2023
SRR3199178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14146698
Total Bases721.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC1870311.3220823686205785No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA1680741.1880793666479628No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT1634171.1551600239151214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC1308960.9252759901992678Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT1304460.9220950358875265No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC1184830.8375311327067277No Hit
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC865170.6115702759753548Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA750330.5303923219397205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTC738070.5217259886370657Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG700800.49538061814848944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC668420.47249188467867204Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT658380.46539482216980954Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT638080.4510451838301772Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG622100.43974926162981637TruSeq Adapter, Index 11 (100% over 49bp)
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACG597610.42243780138658504No Hit
AACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTCC559710.3956470972943651Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACT532850.3766603344469501Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACAC528720.3737409252675077No Hit
GGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCT523350.36994498645549656Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACT520120.36766176813840235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCC519960.3675486675406515Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG514510.36369617842976504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTC477480.337520458837815No Hit
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC454270.32111380337659007No Hit
GTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTCC445480.3149003392876557Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGT412800.29179954219705545No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTC383810.271307127642083No Hit
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC360920.25512667337635964No Hit
CGTGCAAAGTCGTACCCATATCCGCAGATCGGAAGAGCACACGTCTGAAC360590.2548934033934986Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAAC355330.2511752212424412Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAA347020.2453010589467592Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAAC325340.22997592795152622Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAAGATCGGAAGAGCACACGT320870.22681618000186332No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG320380.22646980942125153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCAC313060.22129545707415257No Hit
AAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTGA307980.21770451309556477Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACG303760.21472148482988754No Hit
CCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGT289290.20449294952079983No Hit
GTGTGAGTCCCATCTGACGAGCGAGATCGGAAGAGCACACGTCTGAACTC288200.20372245169862252Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACT287920.20352452565255866Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTAATGGCTATCACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAG284080.2008101113065395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGTAACGGTTAGCACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG281560.1990287768919645Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAACT276410.19538835140186073Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACG266260.18821353223204454No Hit
GTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACACGTCTG255230.180416659774599Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTG250190.1768539909454489Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCCA236390.16709906438944266Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA230050.16261745320356735Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC219890.15543556524639177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGCAAAGTCGTACCCATATCCGCAGAGATCGGAAGAGCACACGTCTGA216220.1528413202854829Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGA213590.15098222920995413Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACTC208540.14741249159344463Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT207940.14698836435187915No Hit
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC207750.14685405739205007No Hit
TAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGA204190.14433756909209486Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT203770.14404068002299902Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGAAC198880.14058404300424027Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACGAAACCTAGAAGGCAGAGTGAGATCGGAAGAGCACACGTCTGAACTC197370.13951665611296712Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTGAACATGGGTCAGTCGATCCTAAGCTAGATCGGAAGAGCACACGTCT191330.13524710854787456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGA190990.1350067697776541Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGA190920.13495728826613815Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAAC190300.1345190234498538Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGGTCAGTCGATCCTAAGCTATAGGAGATCGGAAGAGCACACGTCTGA186330.13171271486816216Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTG184470.13039792041930914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACAC180860.1278460881825568No Hit
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG179610.12696248976262872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGAGTCCCATCTGACGAGCAGATCGGAAGAGCACACGTCTGAACTCC176570.12481357840536357Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGAGATCGGAAGAGCACACGTCT176460.1247358217444099Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACTC175300.12391584241071663Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTG173600.12271414855961439Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAGTGGAGAAGGTTTCCATGTGAACAGATCGGAAGAGCACACGTCTGA170600.12059351235178697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA170290.1203743799436448Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC169760.11999973421359528Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGT169220.11961801969618635No Hit
GGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCTGAACTCCA169120.1195473318225921Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACG166440.11765289681026625No Hit
ATGGGTCAGTCGATCCTAAGCTATAGGTTAAAAGATCGGAAGAGCACACG164730.11644413417180462No Hit
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGC159900.11302990987720243No Hit
ATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGAA159280.11259164506091811Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTG156360.11052755915196608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC155220.10972171739299164Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGCAAAGTCGTACCCATATCCGCAGCAGATCGGAAGAGCACACGTCTG153470.10848467960509231Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAACT153370.10841399173149804Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGGTCAGTCGATCCTAAGCTATAGAGATCGGAAGAGCACACGTCTGAA152120.10753039331156994Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCT150980.10672455155259554Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTCCGATCACTGAAGTCAAACTACGACGAGATCGGAAGAGCACACGTCT150940.10669627640315783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTACAATGAGTCGATCAACGTGCAGATCGGAAGAGCACACGTCTGAACT150600.10645593763293738Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAACT150530.10640645612142142Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAA149660.10579147162115145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGAACTCCAG148440.10492907956330164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC145890.1031265387866483Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAGATCGGAAGAGCACACGTCT145810.10306998848777289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG145300.10270948033244225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTG145090.10256103579789433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTTAATATTCCCGCACTTTGTGACAGATCGGAAGAGCACACGTCTGAAC144430.1020944958321723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTACAATGAGTCGATCAACGTGAGATCGGAAGAGCACACGTCTGAACTC142150.10048281231422344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAA141910.10031316141759725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)