FastQCFastQC Report
Thu 28 Sept 2023
SRR3199179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11653006
Total Bases594.3 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC1530961.313789763774257No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA1366041.1722640492933754No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT1233881.058851252629579Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC1050440.9014326432166944Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT995800.8545434542812387No Hit
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC960920.8246112633941834No Hit
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC692210.5940184017754732Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTC602950.5174201403483358Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA602800.5172914181971587Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG556540.47759350677413187Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC526480.45179758767823514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT515990.44279561857258115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT490270.42072406038407606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACG488010.4187846466396739No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG482030.4136529235460791TruSeq Adapter, Index 11 (100% over 49bp)
AACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTCC462200.39663585516046246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACT438030.3758944258674543Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACAC433170.37172382816931526No Hit
CGTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACT430880.36975866999467777Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCC423750.36364007707539153Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCT403930.3466315901665201Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG399250.3426154590497937Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTC392090.33647112170027205No Hit
GTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTCC346310.29718512116101203Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC339120.29101503938125495No Hit
CGATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGT338020.2900710769392893No Hit
CGTGCAAAGTCGTACCCATATCCGCAGATCGGAAGAGCACACGTCTGAAC297010.254878440807462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTC289070.2480647482718193No Hit
GCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAAC272580.23391389311908017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAA271320.2328326270491923Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC270510.23213752743283578No Hit
CAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAAC269080.23091037625828048Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACG247160.21209977923292925No Hit
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG244020.20940519553495468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTGA243190.2086929329651079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCAC239100.2051831089763448No Hit
GGGCGACCGGGGTTCGATTCCCCGACACGGAAGATCGGAAGAGCACACGT237160.20351830248778727No Hit
CAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACT234230.20100392980146067Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGT233520.2003946449525556No Hit
CTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAACT229250.19673035438237996Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTGAGTCCCATCTGACGAGCGAGATCGGAAGAGCACACGTCTGAACTC228390.19599234738229776Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACG220870.189539076869951No Hit
GTGTAATGGCTATCACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAG205600.17643516188011915Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGTAACGGTTAGCACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG202090.17342306354257433Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTG199460.171166135158602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACACGTCTG198930.17071131689110947Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC195290.16758765935587777Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCCA182880.1569380467151566Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGTGCAAAGTCGTACCCATATCCGCAGAGATCGGAAGAGCACACGTCTGA179080.15367708555200263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGA178740.1533853153426678Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC171770.14740402605130384No Hit
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA168190.14433185737654303Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGA165200.14176599582974556Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACGAAACCTAGAAGGCAGAGTGAGATCGGAAGAGCACACGTCTGAACTC164590.1412425257482919Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACTC164050.14077912600405423Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGAAC163740.14051310022495483Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT158270.13581903244536217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT156410.13422287777076575No Hit
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACAC148710.12761514067700644No Hit
ATGGGTCAGTCGATCCTAAGCTATAGGAGATCGGAAGAGCACACGTCTGA147190.12631075621174487Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGA146380.12561565659538834Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACATGGGTCAGTCGATCCTAAGCTAGATCGGAAGAGCACACGTCT144980.12441424985106847Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGAGATCGGAAGAGCACACGTCT143630.1232557504904743Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTG142950.12267221007180465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGA142320.1221315770368607Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACTC141610.12152229218795561Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGTGAGTCCCATCTGACGAGCAGATCGGAAGAGCACACGTCTGAACTCC140530.12059549269948029Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAAC140270.1203723743041066Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAGTGGAGAAGGTTTCCATGTGAACAGATCGGAAGAGCACACGTCTGA139510.1197201820714758Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTG138280.11866466043182335Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGT138190.11858742714111706No Hit
CGTCCGATCACTGAAGTCAAACTACGACGAGATCGGAAGAGCACACGTCT135360.11615886922224188Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACG133110.11422803695458493No Hit
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC130220.11174799017523891Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA128860.11058090933789959Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG127870.10973134314013054Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGAA127380.10931085077961857Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCTGAACTCCA127280.10922503601216715Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC126710.10873589183769408Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGGTCAGTCGATCCTAAGCTATAGGTTAAAAGATCGGAAGAGCACACG126310.10839263276788838No Hit
CGTGCAAAGTCGTACCCATATCCGCAGCAGATCGGAAGAGCACACGTCTG125560.10774902201200275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAACT124180.10656477822117315Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGC124170.10655619674442801No Hit
CAAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAA122620.105226067848931Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAA122330.10497720502332189Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGGTCAGTCGATCCTAAGCTATAGAGATCGGAAGAGCACACGTCTGAA121950.10465110890700649Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCT121870.10458245709304534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC121610.10435933869767165Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTG119950.1029348135579781Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAGATCGGAAGAGCACACGTCT119550.1025915544881724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGAACTCCAG118900.10203375849973817Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTCCGATCACTGAAGTCAAACTAAGATCGGAAGAGCACACGTCTGAACT118710.10187071044158047Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG117520.10084951470890859Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAACT117240.10060923336004463Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)