FastQCFastQC Report
Thu 28 Sept 2023
SRR3199180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3199180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11525829
Total Bases587.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTC1514481.31398791358088No Hit
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACA1362591.1822056357074184No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCT1258551.0919388097810578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGT1037580.9002215805908625No Hit
AAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTC1035940.8987986894478479Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCGAGATCGGAAGAGCACACGTC958560.8316625207609795No Hit
AAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAAC685830.5950374589107646Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTC595950.5170560833411636Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAA595370.5165528657418048Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTG554140.48078103535979927Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAAC537070.4659708208407395Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCCAGATCGGAAGAGCACACGTCT509020.44163417659588733Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGATCGGAAGAGCACACGTCT499490.43336579086849203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACG487780.423206001060748No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG474520.41170140559954516TruSeq Adapter, Index 11 (100% over 49bp)
AACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACTCC453020.3930476497612449Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACT428390.37167825411950844Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACT427310.37074122824484035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACAC425790.36942245108790006No Hit
GGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCT416860.36167463529087585Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCC416000.3609284850573438Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTGTTAGTATAGTGGTGAGTATTCCAGATCGGAAGAGCACACGTCTG399200.34635252700695107Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTC391850.33997554535990426No Hit
GTGCAAAGTCGTACCCATATCCAGATCGGAAGAGCACACGTCTGAACTCC351540.30500192220446787Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGCGACCGGGGTTCGATTCCCCGACACGGAGATCGGAAGAGCACACGTC349670.30337947925481107No Hit
CGATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGT335830.2913716661942495No Hit
GATAGCAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTC294340.2553742555090831No Hit
CGTGCAAAGTCGTACCCATATCCGCAGATCGGAAGAGCACACGTCTGAAC294120.25518337986794704Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAAC278960.24203031296056882Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAAGATCGGAAGAGCACACGTC273510.2373018027596974No Hit
TGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAA271770.23579214996162098Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAAC265150.23004852839652576Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTTAGCGTCATACCGCGTAGAATAAACAGATCGGAAGAGCACACGTCTG253010.21951566347201576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACG248330.21545521801512066No Hit
GGGCGACCGGGGTTCGATTCCCCGACACGGAAGATCGGAAGAGCACACGT246660.21400629837558757No Hit
GAAAGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCAC245460.2129651585148452No Hit
AAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTGA241960.20992850058768006Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGT234830.20374239458176932No Hit
GTGTGAGTCCCATCTGACGAGCGAGATCGGAAGAGCACACGTCTGAACTC234510.2034647572855714Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAACT228530.19827641031287205Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGAACT227470.19735673676921633Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACG218430.18951348315162406No Hit
GTGTAATGGCTATCACTGTGAGATCGGAAGAGCACACGTCTGAACTCCAG211600.18358766211089894Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGTAACGGTTAGCACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAG209020.1813492114103029Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACACGTCTG202220.1754494188660963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTG195660.1697578542940382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCCGATCACTGAAGTCAAACTACAGATCGGAAGAGCACACGTCTGAAC194230.1685171626266536Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGCAAAGTCGTACCCATATCAGATCGGAAGAGCACACGTCTGAACTCCA188790.16379732859128832Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGA179280.1555462951949053Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGCAAAGTCGTACCCATATCCGCAGAGATCGGAAGAGCACACGTCTGA176720.15332519682532164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGACCTCAGATTAGTCGAGAGCACTCGCTGAGATCGGAAGAGCACACGTC173830.15081778499403384No Hit
GGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGA170280.14773774623933775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCAAAGTCGTACCCATATCCGAGATCGGAAGAGCACACGTCTGAACTC167220.1450828395944448Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTTAGCGTCATACCGCGTAGAATAAACCAGAGATCGGAAGAGCACACGT164660.14286174122486114No Hit
TAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACGTCTGA164470.1426968940802436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACGAAACCTAGAAGGCAGAGTGAGATCGGAAGAGCACACGTCTGAACTC162190.14071872834483315Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAGTGGGTTAATATTCCCGCACTTTGTGAGATCGGAAGAGCACACGTCT159330.1382373450100639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTGAAC159050.13799441237589072Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGAACATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGA152460.13227681930731403Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACATGGGTCAGTCGATCCTAAGCTAGATCGGAAGAGCACACGTCT150680.1307324618472129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAAGCAGATCGGAAGAGCACAC148200.12858077280167873No Hit
ATGGGTCAGTCGATCCTAAGCTATAGGAGATCGGAAGAGCACACGTCTGA147760.12819902151940654Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGA145480.1262208557839961Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAAC143990.12492810712357437Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCTG142380.12353124447707839Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGTGAGTCCCATCTGACGAGCAGATCGGAAGAGCACACGTCTGAACTCC142140.12332301650492992Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACAAGTACCGTGAGGGAAAGTTAGATCGGAAGAGCACACGTCTGAACTC140760.12212570566507623Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTG140350.12176998287932261Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAGTGGAGAAGGTTTCCATGTGAACAGATCGGAAGAGCACACGTCTGA140230.12166586889324837Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGATAGCAAACAAGTACCGTGAGGGAAAGAGATCGGAAGAGCACACGTCT139580.12110191813534626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGT137260.11908904773791108No Hit
GCTTAGCGTCATACCGCGTAGAATAAAAGATCGGAAGAGCACACGTCTGA136080.11806526020818112Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTCCGATCACTGAAGTCAAACTACGACGAGATCGGAAGAGCACACGTCT133470.11580078101106654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTTAATATTCCCGCACTTTGAGATCGGAAGAGCACACGTCTGAACTCCA132080.11459479400570667Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAGCTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTG131420.11402216708229838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTGAAC130790.11347556865540864Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGATAGCAAACAAGTACCGTGAGGGAAAGTAGATCGGAAGAGCACACG129900.11270338992535808No Hit
GTCTAGGGGTATGATACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC128240.11126314645133117Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGGTCAGTCGATCCTAAGCTATAGGTTAAAAGATCGGAAGAGCACACG127630.11073390035545383No Hit
GTGCGAAAGGGAAGTGGGTTAATATTCCCGCACTTTGAGATCGGAAGAGC127420.11055170087982391No Hit
CGTGCAAAGTCGTACCCATATCCGCAGCAGATCGGAAGAGCACACGTCTG124960.10841736416530212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCAAACAAGTACCGTGAGGGAAAAGATCGGAAGAGCACACGTCTGAA123790.10740225280107835Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAACT123630.10726343415297937Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGAAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTG122910.10663875023653396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAACAAGTACCGTGAGGGAAAGTTGAGATCGGAAGAGCACACGTCTGAA121450.10537203007263077Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGGTCAGTCGATCCTAAGCTATAGAGATCGGAAGAGCACACGTCTGAA121020.10499895495586478Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAAGGCTGAAGTGGAGAAGGTTTCCATGAGATCGGAAGAGCACACGTCT120650.10467793683213589Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCTACAATGAGTCGATCAACGTGCGAGATCGGAAGAGCACACGTCTGAA120530.10457382284606166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGCAGTTGAACATGGGTCAGTCGATCCTAGATCGGAAGAGCACACGTCT118730.10301211305494816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCGGCGACAATGAAATACCACAGATCGGAAGAGCACACGTCTGAACTC118030.10240478146951511Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTACAATGAGTCGATCAACGTGCGTATAGATCGGAAGAGCACACGTCTG117770.10217920116635429Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGTGGAGAAGGTTTCCATGTGAAAGATCGGAAGAGCACACGTCTGAACT117240.1017193643945264Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGGTCAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGAACTCCAG116990.10150246025687176Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGTCCGATCACTGAAGTCAAACTAAGATCGGAAGAGCACACGTCTGAACT116640.10119879446415525Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCATACCGCGTAGAATAAACCAGTTCTAGATCGGAAGAGCACACGTCTG115640.10033117791353663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTACAATGAGTCGATCAACGTGAGATCGGAAGAGCACACGTCTGAACTC115620.10031382558252425Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)