FastQCFastQC Report
Tue 4 Jul 2023
SRR3208865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39165304
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG13779013.5181675086704294No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC8527292.177256175516983Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG7715111.9698838543420982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA7013611.7907712397687503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT6058431.5468870099923138Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4842651.2364642950301115No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA4292081.0958883403534925Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3547810.9058553458438623No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG3503320.8944958016922325No Hit
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC3252270.8303956992137734Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG2987070.7626827050799861Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2597070.6631047725302988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2340000.597467595298124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2334140.5959713730295569No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2156280.5505587292262559Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1804930.4608492251202748No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT1803560.46049942571619007Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1690750.43169587040611257Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT1601490.40890529025384303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1568770.40055095704095645No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA1381770.35280461502354227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1371280.35012622396598786No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1353310.3455379792277369No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1239530.316486755726446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1198990.3061357573019221No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1181640.30170581594362195No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC1170780.2989329535141614No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC1142670.29175568252961853No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT1131700.2889547340166184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT1125870.2874661715890166No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG1095280.2796556870846707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1065810.2721311699763648No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT1057270.2699506685815588No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1023620.26135888029874604No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1013760.25884134590146424Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1009440.2577383288024523No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC997160.254602900567298Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC932790.23816743513595606Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC915900.23385494467245807No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG877700.2241014138432323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC860910.21981445618295212Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA856320.21864250051525197Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT847930.2165002983252728No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT824610.21054604861486587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG807660.20621823846943715No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT797290.20357048677574416No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT779650.19906650028811215No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT737580.18832484997435486No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT736780.1881205875486119No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT654780.1671836889099597No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT643890.1644031666395338No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC627250.16015450818408047No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT592260.15122058033814828Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC573670.1464740322199465Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG561390.14333860398479226No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTC540820.1380865063628767No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC531690.13575536142908531No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC502350.12826403696496266No Hit
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT497460.1270154828876089No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA493360.12596863795567628No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG475510.12141103258128674No Hit
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA461900.11793601806333483Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC459350.11728493158127919Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC456190.11647809499959455No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC450270.11496655304909671Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC438220.11188985026134356No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG435640.11123110393832256No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC432630.1104625665614647No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT432220.11035788206827145No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT421790.10769481069264775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG415450.10607603096863488No Hit
TAAAATTGGAACGATACAGAGAAAGATCGGAAGAGCACACGTCTGAACTC413910.10568282579907973Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA413700.10562920691232221Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGACAGTCTCTACGGAGACTGAGATCGGAAGAGCACACGTCTGAACTC411830.10515174349214804Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC403510.10302741426442139No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGTC401530.10252186476070758No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT398990.10187333155897373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA398000.10162055680711683Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG396820.10131926972914598No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT395860.10107415481825444No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG392720.10027242479721336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG392480.1002111460694905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)