FastQCFastQC Report
Sun 2 Jul 2023
SRR3208868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39809441
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG27169246.824823287521169No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT16616444.17399480691025No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC9029042.2680650049821094No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7459441.8737866728648613No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA6343561.5934813051004661Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6193601.555811848752159No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT5370331.3490091458455797No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT4602991.1562558740777094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT4152601.0431193947184538No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4130971.0376860102104926No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA3012140.7566396121965139Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTC2798000.7028483519776125No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2396970.602110941472401No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2294340.5763306246877469Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2101740.5279501412742771No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2091830.5254607820290669Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC2081710.5229186714779542No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGT2054190.5160057384377741No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1920680.4824684677184992No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1831420.4600466507429733No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG1812070.45518599469909665Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC1806210.4537139820677211Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1768070.44413334012904127No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1717060.4313197967286202Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1657840.4164439284641048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1606950.4036605286670566Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTCT1485970.37327075253329983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1428830.3589173733939143Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1272560.3196628659015835No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT1210060.3039630724782094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1007620.2531108135881637No Hit
AGTCCGCCCGGAGGATTCAACCCGAGATCGGAAGAGCACACGTCTGAACT924210.2321584972770655Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG887560.22295213841359895No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG874670.21971421301796223No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA873680.21946552829013602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT851870.21398692837711536Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC836040.21001048469884318No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT796090.19997517674262244No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT771420.1937781542825482No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT767860.19288389404915282No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT766750.1926050657179537No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGAGATCGGAAGAGCACACGT762500.19153747976516425No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT718020.18036425078161736No Hit
TGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAACT696630.17499115348040178Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG673430.16916339016164533Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGTGGCGGGAGATCGGAAGAGCACACG659290.16561146889754116No Hit
TTGGATAACTGTGGTAATTCTAGAGAGATCGGAAGAGCACACGTCTGAAC621950.15623178431468054Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTG615610.15463919726981346Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACACG596700.14988906777163738No Hit
GTCCGCCCGGAGGATTCAACCCGAGATCGGAAGAGCACACGTCTGAACTC555290.13948701264104663Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC526440.13223998799681713No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC524220.1316823313344189No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGAGATCGGAAGAGCACACGTC517040.12987873906594166No Hit
ATGGTGACCTCCCGGGAGCGGGGGACCACCAGAGATCGGAAGAGCACACG507630.12751497816811846No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT495790.12454080930199447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCGCCCGGAGGATTCAACCCGGTGGCGGGAGATCGGAAGAGCACACGT461080.11582177202638941No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG456980.11479186557781607Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC452990.11378959076566786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC448910.11276470825099No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT446980.11227989863007622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT434260.10908467667255112No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT423840.1064672071130062No Hit
GACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAACT422800.10620596255044124Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG420550.10564076998719976TruSeq Adapter, Index 7 (100% over 49bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC419200.10530165444925489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)