FastQCFastQC Report
Sun 2 Jul 2023
SRR3208874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50196119
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG9993211.99083319568989No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC7285451.4513970691638531No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA7101801.4148105752956717Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6796191.3539273823141584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT6295401.254160705133399No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC5450281.0857970912053978No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5146731.0253242885172058No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG5078191.0116698464277687TruSeq Adapter, Index 5 (100% over 49bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG4374590.8714996472137616Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC4255410.8477567757778246Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT3934670.7838594055448789Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG3704590.7380231925898494No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT3544070.7060446246850279No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG3502990.6978607250492812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT2975840.592842645862721No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2828800.5635495445374971No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC2526390.5033038510407548No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2400150.4781544963665418Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT2342190.4666077869486284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2297850.4577744347127713Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2234130.4450802262222703No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2182680.4348304298186878No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2088000.41596841381302807Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1934220.38533257919800534No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1915190.38154144944950824No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1890320.37658688314130423No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGTC1790400.35668096172933206No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1774350.35348350337602796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC1682780.33524105718213No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT1623950.32352102759179446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1562130.3112053344203762Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT1514840.30178428734699586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC1454220.2897076564823667No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1266730.25235616323246024No Hit
CTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAACT1185640.23620152785118706Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA1100960.21933169773543648Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1086870.2165247078165545No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTC1084610.21607447380543504No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC992890.19780214482318842No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC982440.19572031056823339No Hit
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC926990.1846736398086872Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT907470.18078489295158456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG895220.17834446523644587No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA805700.16051041714997927Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT793640.15810784096674885No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT792230.15782694275627165No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT780040.1553984681564724No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC755970.15060327671946114No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT727060.1448438673117338No Hit
CCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTCT714350.14231179904565927Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTGA714310.14230383030209964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTGAA696330.13872188007204303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTGAA675130.13449844598543564Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAAC665870.1326536818513798Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC661240.13173129978435186Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC641870.12787243571559787No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC639700.12744013137748758Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACACGT631660.12583841392200062No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG629190.12534634400719308No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG618390.1231947832460912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG607690.12106314434388842Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC603010.12013080134741096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG581990.11594322660682192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT581230.11579182047918885No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGATCGGAAGAGCACACGTCTGAAC550620.10969373947017697Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC541290.10783503003489174No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC532290.10604206273397351No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCTG516980.10299202613652263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA509010.10140425398226505Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT507350.10107355112454013Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)