FastQCFastQC Report
Sun 2 Jul 2023
SRR3208885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29761657
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG15232765.118249968407337No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT12412954.170785920958635No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC6804392.2862940729408985No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4571501.5360367871990461No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA4513091.5164108638171592No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4210191.4146356165585807Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG3518841.1823400827447208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC3300581.1090041122374334No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA2965980.9965775763090073Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG2370760.7965819913857619No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2110920.7092750245727246No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT1652050.5550934210417114No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1577370.530000732150095No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1572490.5283610384999733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1509580.5072231025308839Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1367940.459631666341696No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1346920.45256888754547503Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1337850.4495213421752693No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG1314540.441689116973561Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC1259260.4231148823467726No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1243370.41777579789996233No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT1200400.4033377577061653Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1077800.3621438147748292Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1065120.35788329930689006No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT919320.3088940914815328Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC918410.3085883289361207No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT901050.3027553203774911No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT876230.294415730952077Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG868510.29182178935803205No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC846880.2845540488555459No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT810710.2724008276824103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG777590.2612724150406007No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG766480.25753942396419666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGT727820.24454955582614235No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT719130.24162969151885597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC695460.23367650531017142No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT685130.23020559641554905No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG680730.22872718410806228No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT657990.2210864805007329Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT650060.21842197831928511No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC624140.20971278581699937No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC622520.20916846128560654No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG620390.20845277532766404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT613500.2061377160552586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA609850.2049113058456389Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACACG605830.20356057460107144No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT593810.19952182097925528No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA548700.18436473479954427No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC526520.17691219275862227No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC523950.17604866556993115No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT522750.17564546221334382Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT518700.17428465088486167No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA485130.16300503698433189Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG476050.15995413158615462No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG442680.14874171824505605No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC441300.14827803438498063No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG440860.14813019315423195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTC420000.14112117480555603No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT417900.14041556893152823No Hit
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC382480.1285143498562597No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT377080.1266999347516168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC374880.1259607285978734No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG362840.12191525492011417No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA360350.1210786079551955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACAC338950.11388814809605527No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT336230.11297422048779071Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG321890.10815594037657243Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC306380.10294453699268157No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG303860.10209780994384823No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT302310.10157700560825629No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC298430.10027331475529068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)