FastQCFastQC Report
Sun 2 Jul 2023
SRR3208886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27716403
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC6146162.2175171864833976No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC5395061.9465224257274654Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC3981821.436629421213135Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT3853211.39022729608889No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT3689111.3310204791004085No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC3584281.2931981108804054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGT3469451.2517677708756074No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA3184441.148936967037173No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT3175651.1457655598383383Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC3133381.1305146631040108No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT2935671.0591814529468344No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC2767240.9984123841755368No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2683460.9681847965625265Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACG2549140.9197225195491636No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2532980.9138920371449354Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC2319140.8367391684988849No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCT2158840.7789033807886254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCT2127130.7674625022590413Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCTG1981280.7148402337778103Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT1961990.7078804562049411Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1945120.7017938078039925No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAAC1687480.608838022740541Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTC1644960.5934969267115939No Hit
CTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTCTGAACT1467540.5294842913057657Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1455600.5251763729947209No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1441880.5202262357059825No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT1421900.5130175080799626No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA1384970.4996932682787157No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACAC1372050.4950317687327609No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGT1326060.47843870649448994No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC1281270.4622786008703943Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT1145230.4131957527100469Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC1113670.40180899375723467No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACAGATCGGAAGAGCAC1108570.3999689281469893No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1100040.3968913282145594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC1091500.39381012031034474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1083600.39095982260035694Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAAC1058480.38189659747695254Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCTGAACT993480.3584447808757868Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAA961890.34704719800762024Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT954630.3444278104918593No Hit
GGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCTGAA925070.33376264589600607Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT851730.3073017808263215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACAC829260.29919466822588775No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC816650.2946450158052616Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC750480.2707710665052749No Hit
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA742460.2678774731338695Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTG736350.26567300237335995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC726870.26225264512137453No Hit
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC700390.2526987358352381Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG671050.24211294661865032Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGTCTGAAC654000.23596135472557533Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT652900.23556447782924791Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAGATCGGAAGAGCACACGT650210.23459393341913812No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT649980.23451095006808784No Hit
CGGTCGGCGGGCGGCGGGGCGGGGCAGATCGGAAGAGCACACGTCTGAAC647300.23354401362976285Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG617390.22275257002144183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT616990.22260825115005003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTCTGAACTC611050.22046511590988194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG587980.21214152500236053No Hit
AGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACGTCTGAACT582920.21031589127925437Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTC581400.20976747956796557No Hit
GGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACACGTCTGA573780.207018205067952Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCT566600.20442768132646938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAAC561280.20250824033695858Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC561170.20246855264732583No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG551680.19904458742355563TruSeq Adapter, Index 1 (100% over 49bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG545260.1967282695377174No Hit
CGGTCGGCGGGCGGCGGGGCGGGGAGATCGGAAGAGCACACGTCTGAACT541630.19541857577983693Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTCTGAACTC530780.19150392639333466Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCTGAACTC527830.19043957471682021Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACA511230.18445034155406098No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG502410.181268110439872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACT500240.18048518056257157Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACA498170.17973833040311904No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCTG489880.17674732179352423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC477930.17243579551069452Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGTCTGAACTCC474740.171284852511345Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG468870.16916697307367048Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGTGTAGTGGTATCATGCAAGAGATCGGAAGAGCACACGTCTGAACTC467350.16861856136238168Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGGAGATCGGAAGAGCACACGT452040.16309475655986097No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG442540.15966718336430596No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAGATCGGAAGAGCACACGTC438800.15831780191679273No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTCT433100.15626125799945975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG432960.15621074639447263Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAGGTGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG432140.15591489270811945No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT431310.1556154310499815No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGT430570.1553484411379067No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC428050.15443923224813838No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC426210.1537753654397362No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC419860.15148430335639151No Hit
GTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTGAACT416810.15038387196202913Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGAGATCGGAAGAGC402480.14521364839441828No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACA401550.14487810701843237No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTCC399010.1439616821850945Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC397010.14324008782813555No Hit
CCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT388350.1401155842625033No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG387000.13962850807155605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGC382760.13809872803480308No Hit
AGGTGGCCCGGGTTCGACTCCCGGTATGGGAAGATCGGAAGAGCACACGT372970.13456652365748903No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTC365390.13183168104461462No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAGATCGGAAGAGCACAC363270.13106679102623814No Hit
CTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTCTGAACT359630.12975348929657288Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAACTC354980.12807578241664333Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGTC354260.1278160084481381No Hit
CGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGA349620.12614190953999332Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA345380.12461212950324037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC342420.12354416985494113No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCT340090.12270351242908396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC335430.12102219757736962No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA319310.11520614706028053Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGTC314720.11355008801105973No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA304730.1099457241980498Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGT302150.10901486747757275No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTG297310.10726860913373212Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT296620.10701965908058127No Hit
GTCTAGCGGTTAGGATTCCTGAGATCGGAAGAGCACACGTCTGAACTCCA291160.10504970648608336Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC286080.10321685681940762Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT280340.10114588101493545Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)