FastQCFastQC Report
Sun 2 Jul 2023
SRR3208905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23241417
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT7695953.3113084283974596No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC7279883.1322875020916325No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT6378352.744389466442601Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC5906762.541480151575956No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG5225142.248201992159084No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT4149131.7852310812202197No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2933451.2621648671421368TruSeq Adapter, Index 5 (100% over 49bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT2393291.0297521876570606No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG1995560.8586223464774114No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG1834030.7891214206087349Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1644620.7076246684959011No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1414090.6084353634720293No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1373170.5908288638339048Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG1239800.5334442387914644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1224740.5269644273410696No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1055960.4543440703292747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG917370.3947134548637891Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA911200.39205871139440424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT827960.35624333920776No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT817800.35187183294374863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT790640.3401857984820805No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG784150.33739336977603385No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT780940.3360122147457705Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT757800.32605585106966584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA746250.32108627455890487Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC671350.2888593238527582No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC615770.26494511931006615Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT566710.2438362514643578No Hit
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA550150.23671104046711094Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC547140.2354159387097611No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA536250.2307303379996151Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA525670.22617811986248515No Hit
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC521610.2244312384223389Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC501740.21588184575837177No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT497850.21420810960020206Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT465730.20038795397027642No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGAGATCGGAAGAGCACACGTC458510.1972814308180951No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG454180.19541837745951549No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG425810.18321172069672No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA411070.176869594482987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT383660.16507599343017682No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC371540.15986116509161208No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT363340.1563329809021541No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG361050.1553476709272933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTC347970.14971978687874324No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT340780.14662617171749898Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC315830.13589102592152622No Hit
TGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA314090.1351423624471778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTAGATCGGAAGAGCACAC313980.13509503314707533No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT309760.13327930908859817No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC305680.13152382232116056No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC303570.13061596029192196No Hit
AGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACGTCT288460.12411463552329878Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC287100.12352947326748623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG285050.12264742722012173Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCTGAACTCCA283010.12176968383640291Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC277550.11942042948586137Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG272000.11703245116250872Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC264770.11392162534668174No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC262180.1128072354624505No Hit
TCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTC261040.11231673180684293No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC256350.11029878255701879No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACAC256200.11023424260233358No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT254490.10949848711892224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACG249050.10715783809567204No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGT239480.10304018898675583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)