FastQCFastQC Report
Sun 2 Jul 2023
SRR3208906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32511884
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT28434318.745820451377103No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC22524276.928011308111212No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC7768192.389338618457177No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC5667471.7431995020651525No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT5574371.714563819186855No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT5484381.6868847096034176Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC4368421.3436379140624395No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG4134471.2716796110616044No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3929001.2084811818349255No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT3500851.0767908743768893No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3158210.9714017188299515No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2321630.7140865783108724No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT1750660.538467718450275No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT1729890.5320792852238276Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1618540.4978302703097735No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1616810.49729815719076753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1586270.48790466895120566No Hit
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACGT1446150.4448065821100986No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1336590.41110813510530486No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG1287900.396132072813744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG1206350.3710489370594457Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1198020.36848679701244014TruSeq Adapter, Index 8 (100% over 49bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA1148260.3531816242946733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1113530.34249937653566925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1089850.3352158859818767No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG1081450.33263221534624077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1067100.3282184446770295No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1037910.3192401892181948No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG967390.2975496590723564No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCTG962280.2959779261023446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC955160.29378795765880567No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT952280.2929021277265876No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC920360.2830841793111713No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC907100.2790056706649175No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA881410.27110394463759774Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGA866820.2666163548073683Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC826190.2541193860066676No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT790610.24317569538572417Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGAA740970.22790743224846643Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT715530.22008260118054063Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT714890.21988575008449218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT684590.21056608100594848Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT635820.1955654123273816Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA633230.19476878054806052Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCACACG598890.18420648892571098No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG595280.18309612571206269No Hit
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT581580.17888228193727562Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC581130.17874387101036654No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT547490.16839688527432No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTC544910.16760332929337468No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT542860.1669727906263445Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG496670.15276567792872292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGATCGGAAGAGCACA487860.150055899559681No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC481070.14796743246254201No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT472830.14543297460091825No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC468190.14400580415456699No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT459590.14136061755141596No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAGAGATCGGAAGAGCACACG445490.1370237418415986No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT444050.13658082687548959Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT432820.13312670529951448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAGATCGGAAGAGCACAC422050.1298140704488242No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG421790.1297340996910545Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA413050.1270458519106429No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA412930.1270089423301338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAA396390.12192157181663173Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTGA389870.11991615127563816Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT387080.11905800352880196Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA383440.11793841292002641Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGACCGGCGCAAGACGGACCAGAGCGAAAGCAGATCGGAAGAGCACACGT377610.11614522246696007No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG377040.11596990195954195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT372450.11455811050506946No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA371900.11438894159440283Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT352780.10850801509995545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC343470.10564444681212568No Hit
GCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACGTC329320.10129219211042953No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT328290.10097538487772656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)