FastQCFastQC Report
Sun 2 Jul 2023
SRR3208910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29011354
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT22411227.724982432739955No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC14808685.104442901906612No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC8607902.96707971644481No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC5341821.8412860013358907No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT3681911.2691272527300863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG3541911.2208702840963577No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT2821610.9725881804758234No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2620090.903125721053902No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2494370.8597909632208136No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT2357750.8126990556869561No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1939920.6686761327995928No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1852550.6385603374458152No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG1472110.5074254721099885No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1369740.47213928725973975No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1345920.46392870873934394TruSeq Adapter, Index 6 (100% over 49bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1319770.4549149963838296No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1249900.430831322109268Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC1201420.4141206232566739No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1176710.4056032682928208No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1120780.3863246093236462No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG1002970.3457163702183635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG964970.3326180501606371No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG879930.3033053886419779Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGT839550.28938670011747814No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC831870.286739460695285No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT777900.26813639928698263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG674060.23234351626607985Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT667610.23012024878259732No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT659670.2273833892757987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC645090.222357770685229No Hit
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCAGATCGGAAGAGCACACGT635500.21905216833381855No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT635350.21900046443885385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACAC595940.2054161277684592No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT589850.20331694963289199No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACGT575990.19853950973815288No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC536870.18505513393135667No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT534820.18434851403350563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGCGGGGCGCGGGACATGTGGCGTACAGATCGGAAGAGCACACGTCTGA532580.18357640253536597Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTC521970.17991921369819552No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT511140.17618619248174353Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACG492060.16960945704223251No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTG484130.16687604446176488Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG480070.16547659237138673No Hit
GGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTGA463090.15962371146138163Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC460220.1586344436043902No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC458460.1580277845701376Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT447070.15410173547915068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT445070.15341235021295455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT426200.1469080002263941No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGT421050.14513283316593908No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC400830.13816314812469627No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC390460.1345886855194694No Hit
CAGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCT359280.1238411692194718Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT354570.12221766691757992Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA350040.1206562092896457Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT350040.1206562092896457No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAGATCGGAAGAGCACACGTCT347200.11967728221164721Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT341050.11755742251809412No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG339470.11701280815779919Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAAAGATCGGAAGAGCACACGT334570.11532381425561868No Hit
CTCCCGGGGCTACGCCTGTCTGAGAGATCGGAAGAGCACACGTCTGAACT325170.11208370350449688Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGA320170.11036024033900659Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTC312230.10762338083220796No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA311280.1072959228307648No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGAGATCGGAAGAGCACACGTCTG304450.10494167214670505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA300800.10368354403589711No Hit
CCCGGGGAGGTAGTGACGAAAAATAACAAAGATCGGAAGAGCACACGTCT300240.1034905161613622Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGT297080.10240128744077233No Hit
GGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAGATCGGAAGAGCACACG295860.10198076242839269No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)