FastQCFastQC Report
Sun 2 Jul 2023
SRR3208918.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3208918.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32924832
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT17162235.2125489964534975No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC13578274.124021042840856No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG13433614.08008459997609No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT7218642.192460693497236No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC4813771.4620484623885097No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG3673201.1156321162094311No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT3184520.967209187278465No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT2965280.9006211481959878No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2852700.8664281111593828No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2572080.7811976079331248No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT2372210.7204926664470148Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT2278800.6921219825814146No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2278480.6920247915008343Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG2037120.618718418973254No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1979440.6011997266986815Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTG1798910.5463687711451345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1779930.5406041251782241TruSeq Adapter, Index 11 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT1650240.501214402551849Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCTG1620110.49206325487097397Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1532610.4654875687748384No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTG1389260.421949001896198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT1379430.4189634133896264No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1291110.39213867514950423Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC1273170.38668990019447935No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAAAGATCGGAAGAGCACACGTCT1261680.38320013295739824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT1186370.3603268195871129Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT1184810.35985301306928463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTC1165220.3539030966050184No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1158690.3519197911169296No Hit
TTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTC1105780.335849853387255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAA920510.2795792549526145Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC868990.26393149097920987No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGT814360.24733915119141686No Hit
CCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGTCT812300.2467134836101821Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACT730410.22184167864546736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACAC725080.2202228397095542No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT722510.21944227384364484No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG693550.21064648105114098No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA623390.18933733663394242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCC621160.1886600362911495Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACAC617630.18758789718349966No Hit
CGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA597020.1813281841498842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT585700.17789004967436128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG580680.17636536459776012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC579160.1759037069650044Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC562930.17097429684682977No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC549990.16704413252586983No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGT548890.16671003818637556No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCTG548270.1665217304677515Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC527440.16019519856623718Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT489890.14879043270440986No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCAGATCGGAAGAGCACACG487330.1480129040597686No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC483610.14688305774802435No Hit
CTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAAC478220.1452459954845024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC463550.14079039188415599No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAAGATCGGAAGAGCACAC449550.13653828210877433No Hit
GGCGACCGGCCGCCGCCGGGCGCATTTCCACCAGATCGGAAGAGCACACG448610.13625278330957014No Hit
TGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA420280.12764833545695844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA408910.12419501487509488Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCGGGGCGCGGGACATGTGGCGTACGGAGATCGGAAGAGCACACGTCT399520.12134306410432102Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC398470.12102415587116738No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC392590.11923826976550707Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA382270.11610385741679716Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC379630.11530203100201088Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGTACACGGAGTCGACCCAACGCGAAGATCGGAAGAGCACACGTCTGAA371210.11274469069424561Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA368450.11190641762424179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACTTCCAGGAGATCGGAAGAGCACACGTCTGAAC367310.111560174399675Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA366450.11129897337061584Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGA359020.1090423179683954Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC358550.1088995685687933No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT348450.10583197508798224Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGGGGCGCGGGACATGTGGCGTACGGAAGATCGGAAGAGCACACGTCT337340.10245762225909005Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACAGATCGGAAGAGCACA335030.10175602414615205No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTC332850.10109390990969977No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC331680.10073855502132859No Hit
TTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAAC331310.10062617783440778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)