FastQCFastQC Report
Sun 2 Jul 2023
SRR3284449.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284449.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16458421
Total Bases822.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN8398375.1027799082305645No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN4797682.9150305487993045No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3064481.8619526138017737No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN2358991.4333027451418334No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN2170301.3186562672081361No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN1569960.9538946658370205No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN1463590.8892651366738037No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN1092460.6637696289334196No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN954240.5797883041149574No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN825990.5018646685487022No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN802150.4873796824130334No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN659310.40059128393908505No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN641670.38987336634541064No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN599100.36400818766271686No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN583310.3544143147146376No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN579590.35215407358944095No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN557370.33865338600829326No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN514450.3125755502304869No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN489130.297191328378342No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATGNNNNNNNNNNNNNNNNNNN475410.2888551702499286No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN459820.27938281564191364No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN432260.2626375883810482No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN394570.2397374571959242No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN383280.23287774689929244No Hit
AGAACATTCACGATGTACTTGGGACTGAACNNNNNNNNNNNNNNNNNNNN374210.22736689017737485No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN366070.22242109373675642No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN364610.22153400985428676No Hit
AGAACATTCACGATGTACTTGGGACTGAACTNNNNNNNNNNNNNNNNNNN339940.20654472260735096No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN338820.2058642199029907No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN336470.20443637940723475No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN331400.20135588948660385No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN321740.19548655366149642No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN289340.17580058257107412No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN283580.17230085437722123No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNN249460.1515698255622456No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAANNNNNNNNNNNNNNNNN249340.15149691455820702No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN239820.14571264157114464No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN231920.1409126671386034No Hit
AGAACATTCACGATGTACTTGGGACTGAANNNNNNNNNNNNNNNNNNNNN228600.13889546269353542No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN224480.13639218488821012No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGNNNNNNNNNNNNNNNNN224160.13619775554410718No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACNNNNNNNNNNNNNNNNNN220220.13380384424483976No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATNNNNNNNNNNNNNNNNNNNN219190.13317802479350843No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN217040.13187170263781683No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNN214990.1306261396521574No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNN201810.1226180810419177No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCNNNNNNNNNNNNNNNNNN197100.11975632413340259No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN189050.11486521094581309No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN187020.11363179979416009No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN185560.11274471591169043No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNN184360.11201560587130442No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGANNNNNNNNNNNNNNNNNNN178540.10847942217543226No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN174220.10585462603004263No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN173950.10569057627095575No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCNNNNNNNNNNNNNNNNNNNNNNNN173730.10555690609688499No Hit
TAAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNN173630.10549614692685282No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN169870.1032116021336433No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN165230.10039237664415074No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN165010.10025870647007998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)