FastQCFastQC Report
Sun 2 Jul 2023
SRR3284450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16570003
Total Bases828.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN8444105.0960159753742955No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN4818712.908092412536075No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3084621.8615687637473572No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN2367001.4284849556152768No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN2191921.32282414191476No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN1576940.9516835935394822No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN1473300.8891368335901931No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN1099250.6633975865906603No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN960390.5795955498620007No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN827090.49914897420356535No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN810780.4893058860641124No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN654460.39496673597464044No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN643410.3882980588476659No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN610070.36817736243016974No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN586520.35396493289711534No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN585280.3532165926584322No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN566720.3420156290858849No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN516560.3117440594307678No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN501080.30240187645107847No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATGNNNNNNNNNNNNNNNNNNN478770.2889377871567072No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN463760.27987924926748653No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN432820.26120695331195776No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN396640.2393723163478003No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN385680.2327579542381495No Hit
AGAACATTCACGATGTACTTGGGACTGAACNNNNNNNNNNNNNNNNNNNN374060.22574528200145771No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN371110.22396495643362285No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN371030.22391667641822396No Hit
AGAACATTCACGATGTACTTGGGACTGAACTNNNNNNNNNNNNNNNNNNN344020.20761613621916664No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN338380.20421239513354345No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN338110.20404945008157208No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN333090.20101987911529043No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN325380.1963668926312204No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN287340.17340974530903827No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN282460.1704646643697047No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAANNNNNNNNNNNNNNNNN251650.15187082343919914No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNN248890.1502051629079367No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN238110.14369943083293346No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN235360.14203980530359592No Hit
AGAACATTCACGATGTACTTGGGACTGAANNNNNNNNNNNNNNNNNNNNN228980.13818947407553275No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN224830.13568494827671423No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGNNNNNNNNNNNNNNNNN221270.13353648759146272No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATNNNNNNNNNNNNNNNNNNNN219880.13269762232390664No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACNNNNNNNNNNNNNNNNNN219350.13237776722188885No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNN215980.13034397157320973No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN215420.13000601146541735No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNN205600.12407963957520103No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCNNNNNNNNNNNNNNNNNN199620.12047070842413246No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN192420.11612550703823048No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN187540.1131804260988969No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN187320.1130476560565499No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNN186910.11280022097763047No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGANNNNNNNNNNNNNNNNNNN182210.10996377007294446No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN179260.10818344450510962No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN177270.1069824791220617No Hit
GTGGTCTAGTGGTTAGGATTCGGCGCNNNNNNNNNNNNNNNNNNNNNNNN174220.10514180353497823No Hit
TAAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNN173540.10473142340408749No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN172320.1039951531692541No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN167700.101206982279967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)