FastQCFastQC Report
Sun 2 Jul 2023
SRR3284451.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284451.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14975217
Total Bases748.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAGTCTAACACGTGCGCGAGTCNNNNNNNNNNNNNNNNNNNNNNNNN4520983.0189746165280944No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNNNNNNNNNNNNNNNNN3141682.0979195159575985No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNN2549201.702279172315166No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN2421691.6171318252015983No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN2349231.5687452141761953No Hit
AAGGAGTCTAACACGTGCGCGAGTNNNNNNNNNNNNNNNNNNNNNNNNNN2283881.525106447539291No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCNNNNNNNNNNNNNNNNNNNNNNNN2166401.4466568330862917No Hit
AGCTCTTTCTCGATTCCGTGGGTGGTGGTGCNNNNNNNNNNNNNNNNNNN2083661.3914055469112734No Hit
AGGGAGGACAGAAACCTCCCGTGGAGCNNNNNNNNNNNNNNNNNNNNNNN1412110.9429646328330333No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN1341030.8954995443471704No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCNNNNNNNNNNNNNNNNNNNNN1228500.820355391177303No Hit
ACGAGCGCACGTGTTAGGACCCGAAAGATNNNNNNNNNNNNNNNNNNNNN784750.5240324731187535No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN766410.5117855721222604No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN723890.4833919935851347No Hit
AAGCAGAACTGGCGCTGCGGGATGAACNNNNNNNNNNNNNNNNNNNNNNN686390.45835062022807416No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTNNNNNNNNNNNNNNNNNNNN683560.4564608312520613No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN659930.4406814271873323No Hit
AGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNN651380.43497199406192244No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCNNNNNNNNNNNNNNNNNNNNNNNN629590.4204212867165798No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN623840.4165816094684972No Hit
AGGAGTCTAACACGTGCGCGAGTCNNNNNNNNNNNNNNNNNNNNNNNNNN532310.35546062537858386No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN514540.34359435325711807No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCNNNNNNNNNNNNNNNNNNN462020.30852307515810956No Hit
GAGCGCACGTGTTAGGACCCGAAAGATNNNNNNNNNNNNNNNNNNNNNNN456550.3048703734977597No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN405390.2707072625391672No Hit
AGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNN404530.2701329803768453No Hit
ACGAGCGCACGTGTTAGGACCCGAAAGATGGTNNNNNNNNNNNNNNNNNN383470.25606974509952013No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN376540.2514420993031353No Hit
ATGATTAAGAGGGACGGCCGGGGGCNNNNNNNNNNNNNNNNNNNNNNNNN342080.22843074661288715No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN340770.2275559679702805No Hit
ACCCTGAACGCGCCCGATCTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNN322360.21526232307685425No Hit
GAGCGCACGTGTTAGGACCCGAAAGATGGTNNNNNNNNNNNNNNNNNNNN321770.21486833880270317No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCNNNNNNNNNNNNNNNNNNNNNN315970.21099527305681112No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN312220.20849113572110506No Hit
AGCGTGTGCCTACCCTACGCCGGCNNNNNNNNNNNNNNNNNNNNNNNNNN312090.2084043256268006No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTGCNNNNNNNNNNNNNNNNNN307800.20553959251475287No Hit
AGAAAAGGTGTTGGTTGATATAGACNNNNNNNNNNNNNNNNNNNNNNNNN292070.1950355711039112No Hit
AAGGCGCCCGATGCCGACGCTCATCAGACNNNNNNNNNNNNNNNNNNNNN279810.1868487114410429No Hit
AACTGGCTTGTGGCGGCCAAGCGTTCNNNNNNNNNNNNNNNNNNNNNNNN273870.1828821579012845No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN270500.18063177314893No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN259650.17338646912428715No Hit
TGCACGCGCGCTACACTGACTGGCTCNNNNNNNNNNNNNNNNNNNNNNNN255280.17046831441574437No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN254120.16969370126656594No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNNNNNNNNNNNNNNNNN250250.16710943153611732No Hit
ACCTGCCGAATCAACTAGCCCTGAAAATNNNNNNNNNNNNNNNNNNNNNN243970.1629158362112549No Hit
AGAACTGGCGCTGCGGGATGAACCNNNNNNNNNNNNNNNNNNNNNNNNNN243310.16247510804017062No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCNNNNNNNNNNNNNNNNNN241120.1610126918361183No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCNNNNNNNNNNNNNNNNN240390.16052521976810086No Hit
GAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNNN237060.1583015458139939No Hit
GAAGTGGAGAAGGGTTCCATGTGAACNNNNNNNNNNNNNNNNNNNNNNNN234510.15659873242571376No Hit
ACCCTACTGATGATGTGTTGTTGCCNNNNNNNNNNNNNNNNNNNNNNNNN230960.15422814908124538No Hit
AGGGGAATCCGACTGTTTAATTAAAACNNNNNNNNNNNNNNNNNNNNNNN229950.1535537014254952No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACNNNNNNNNNNNNNNN221800.1481113762825607No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTNNNNNNNNNNNNNNNNNNN220170.14702291125397382No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN215780.14409140114630725No Hit
ACCACCCTGAACGCGCCCGATCTCGTCNNNNNNNNNNNNNNNNNNNNNNN215730.14405801264849785No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNNNNNNNNNNNNNNNNN215200.14370409457171807No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCNNNNNNNNNNNNNNNNN211750.1414002882228685No Hit
AGAACTGGCGCTGCGGGATGAACNNNNNNNNNNNNNNNNNNNNNNNNNNN211640.14132683352768777No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN210690.14069245206930892No Hit
AAGGCGCCCGATGCCGACGCTCATCNNNNNNNNNNNNNNNNNNNNNNNNN205900.13749383397916706No Hit
AGAACATTCACGATGTACTTGGGACTGAANNNNNNNNNNNNNNNNNNNNN198600.1326191132989926No Hit
AGTGGCGTTCAGCCACCCGAGATTGAGCNNNNNNNNNNNNNNNNNNNNNN195380.13046889404006634No Hit
GGCGGGTGTTGACGCGATGTGATTTCNNNNNNNNNNNNNNNNNNNNNNNN184000.12286967193864369No Hit
ACTTGGATGGGAGACCGCCTGGGAATNNNNNNNNNNNNNNNNNNNNNNNN183390.12246233226536885No Hit
GGAGGTCCGTAGCGGTCCTGACGTGCNNNNNNNNNNNNNNNNNNNNNNNN180360.12043898929811835No Hit
AAGGCTAAATACCGGCACGAGACCGATNNNNNNNNNNNNNNNNNNNNNNN177770.1187094651115907No Hit
AGCTCTTTCTCGATTCCGTGGGTGGTGGTNNNNNNNNNNNNNNNNNNNNN176140.11762100008300382No Hit
AGTCAGCGGAGGAAAAGAAACTAACCNNNNNNNNNNNNNNNNNNNNNNNN176100.11759428928475628No Hit
TTGGCTGAGGAGCCAATGGGGCGAAGCNNNNNNNNNNNNNNNNNNNNNNN174860.1167662545390828No Hit
ACCACCCTGAACGCGCCCGATCTCGTNNNNNNNNNNNNNNNNNNNNNNNN174440.11648579115748374No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN172860.11543071462670625No Hit
ACCTGTCCGGTGCCCTGAATGAACTGCAGGNNNNNNNNNNNNNNNNNNNN170550.11388816602791131No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN170260.1136945127406167No Hit
TGATCTCGGAAGCTAAGCAGGGTCNNNNNNNNNNNNNNNNNNNNNNNNNN170040.1135476033502553No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN169270.1130334204839903No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCNNNNNNNNNNNNNNNNNNNNNNNN167980.11217199724050744No Hit
TTGACTCTAGTCTGGCACGGTGAAGAGACNNNNNNNNNNNNNNNNNNNNN165920.11079639113075958No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCNNNNNNNNNNNNNNNNNNNN165180.11030224136318023No Hit
AGGAGTCTAACACGTGCGCGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNN163600.10924716483240278No Hit
AGACGTGGCGACCCGCTGAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNN163460.1091536770385364No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN162820.1087263042665759No Hit
AGCCACCCGAGATTGAGCAATAACNNNNNNNNNNNNNNNNNNNNNNNNNN162390.10843916318541494No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCNNNNNNNNNNNNNNNNNNNNNN159310.10638243172035504No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN156740.10466626293295116No Hit
AGCAGGACGGTGGCCATGGAAGTCNNNNNNNNNNNNNNNNNNNNNNNNNN155740.10399849297676286No Hit
AGTGACGCGCATGAATGGATGAACNNNNNNNNNNNNNNNNNNNNNNNNNN154950.10347095471137412No Hit
AGCAGAACTGGCGCTGCGGGATGAACNNNNNNNNNNNNNNNNNNNNNNNN154870.10341753311487907No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN154370.10308364813678493No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN150650.10059954389976453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)