FastQCFastQC Report
Sun 2 Jul 2023
SRR3284452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15087936
Total Bases754.3 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGAGTCTAACACGTGCGCGAGTCNNNNNNNNNNNNNNNNNNNNNNNNN4547633.014083569813658No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCNNNNNNNNNNNNNNNNNNNNNN3166122.0984447441982788No Hit
AAGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNN2559681.6965077264378638No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN2437611.6156020280043606No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN2378531.5764449159911602No Hit
AAGGAGTCTAACACGTGCGCGAGTNNNNNNNNNNNNNNNNNNNNNNNNNN2286051.5151509126231713No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCNNNNNNNNNNNNNNNNNNNNNNNN2186071.4488860504180294No Hit
AGCTCTTTCTCGATTCCGTGGGTGGTGGTGCNNNNNNNNNNNNNNNNNNN2090621.3856235869505278No Hit
AGGGAGGACAGAAACCTCCCGTGGAGCNNNNNNNNNNNNNNNNNNNNNNN1425310.944668641224353No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN1349130.8941779710624435No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCNNNNNNNNNNNNNNNNNNNNN1248600.8275485792092437No Hit
ACGAGCGCACGTGTTAGGACCCGAAAGATNNNNNNNNNNNNNNNNNNNNN790790.5241207279776372No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN768390.5092744295840067No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN724220.4799993849390665No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGTNNNNNNNNNNNNNNNNNNNN695660.46107035448718764No Hit
AAGCAGAACTGGCGCTGCGGGATGAACNNNNNNNNNNNNNNNNNNNNNNN695410.46090465919261586No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN665700.4412134303857069No Hit
AGAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNN664550.44045123203067665No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN634340.42042861263462417No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCNNNNNNNNNNNNNNNNNNNNNNNN632480.41919583964301016No Hit
AGGAGTCTAACACGTGCGCGAGTCNNNNNNNNNNNNNNNNNNNNNNNNNN535960.3552242003147415No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN525050.3479932576596295No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCNNNNNNNNNNNNNNNNNNN464760.3080341804207017No Hit
GAGCGCACGTGTTAGGACCCGAAAGATNNNNNNNNNNNNNNNNNNNNNNN461180.30566142380243394No Hit
AGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNNNN414370.2746366368468159No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN405890.2690162524549415No Hit
ACGAGCGCACGTGTTAGGACCCGAAAGATGGTNNNNNNNNNNNNNNNNNN387360.25673491722128194No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN381960.25315589885853174No Hit
ATGATTAAGAGGGACGGCCGGGGGCNNNNNNNNNNNNNNNNNNNNNNNNN343190.2274598725763418No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN340400.22561071308892086No Hit
GAGCGCACGTGTTAGGACCCGAAAGATGGTNNNNNNNNNNNNNNNNNNNN325620.21581480727383787No Hit
ACCCTGAACGCGCCCGATCTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNN325600.2158015516502721No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN320460.21239485639387656No Hit
AGCGTGTGCCTACCCTACGCCGGCNNNNNNNNNNNNNNNNNNNNNNNNNN314470.20842479713593698No Hit
GAACCCCATTCGTGATGGGGATCGGGGATTGCNNNNNNNNNNNNNNNNNN313340.20767585440447256No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCNNNNNNNNNNNNNNNNNNNNNN312590.20717876852075723No Hit
AGAAAAGGTGTTGGTTGATATAGACNNNNNNNNNNNNNNNNNNNNNNNNN292640.19395628401393006No Hit
AAGGCGCCCGATGCCGACGCTCATCAGACNNNNNNNNNNNNNNNNNNNNN283350.18779904686764315No Hit
AACTGGCTTGTGGCGGCCAAGCGTTCNNNNNNNNNNNNNNNNNNNNNNNN278190.18437909598768182No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN270060.1789906850082079No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN263870.1748880695146109No Hit
TGCACGCGCGCTACACTGACTGGCTCNNNNNNNNNNNNNNNNNNNNNNNN257060.17037452969047587No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN255750.16950628634691983No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGANNNNNNNNNNNNNNNNNNNN249750.16552959927719735No Hit
AGAACTGGCGCTGCGGGATGAACCNNNNNNNNNNNNNNNNNNNNNNNNNN248360.16460833343937833No Hit
ACCTGCCGAATCAACTAGCCCTGAAAATNNNNNNNNNNNNNNNNNNNNNN244290.16191081404374993No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCNNNNNNNNNNNNNNNNNN241500.16006165455632898No Hit
AGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCNNNNNNNNNNNNNNNNN237770.15758948076131818No Hit
GAGGGCGTGAAACCGTTAAGAGGTNNNNNNNNNNNNNNNNNNNNNNNNNN235650.15618438466334958No Hit
GAAGTGGAGAAGGGTTCCATGTGAACNNNNNNNNNNNNNNNNNNNNNNNN234330.15530951350801064No Hit
AGGGGAATCCGACTGTTTAATTAAAACNNNNNNNNNNNNNNNNNNNNNNN229580.15216130291114702No Hit
ACCCTACTGATGATGTGTTGTTGCCNNNNNNNNNNNNNNNNNNNNNNNNN228580.15149852173285994No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACNNNNNNNNNNNNNNN221900.14707114346190228No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTNNNNNNNNNNNNNNNNNNN220930.14642824571896382No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN220280.1459974379530772No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGNNNNNNNNNNNNNNNNNNNNN218770.14499663837386373No Hit
AGAACTGGCGCTGCGGGATGAACNNNNNNNNNNNNNNNNNNNNNNNNNNN218350.14471827027898315No Hit
ACCACCCTGAACGCGCCCGATCTCGTCNNNNNNNNNNNNNNNNNNNNNNN217690.14428083470131367No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN213310.1413778531404163No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCNNNNNNNNNNNNNNNNN212990.14116576316336443No Hit
AAGGCGCCCGATGCCGACGCTCATCNNNNNNNNNNNNNNNNNNNNNNNNN207210.13733488795286514No Hit
AGAACATTCACGATGTACTTGGGACTGAANNNNNNNNNNNNNNNNNNNNN199770.13240379598640925No Hit
AGTGGCGTTCAGCCACCCGAGATTGAGCNNNNNNNNNNNNNNNNNNNNNN194950.12920919070706555No Hit
ACTTGGATGGGAGACCGCCTGGGAATNNNNNNNNNNNNNNNNNNNNNNNN184830.12250184518280034No Hit
GGCGGGTGTTGACGCGATGTGATTTCNNNNNNNNNNNNNNNNNNNNNNNN181780.12048036258902478No Hit
GGAGGTCCGTAGCGGTCCTGACGTGCNNNNNNNNNNNNNNNNNNNNNNNN181530.12031466729445299No Hit
AAGGCTAAATACCGGCACGAGACCGATNNNNNNNNNNNNNNNNNNNNNNN178920.11858480841912374No Hit
TTGGCTGAGGAGCCAATGGGGCGAAGCNNNNNNNNNNNNNNNNNNNNNNN177300.11751110291029866No Hit
AGCTCTTTCTCGATTCCGTGGGTGGTGGTNNNNNNNNNNNNNNNNNNNNN176970.11729238512146394No Hit
AGTCAGCGGAGGAAAAGAAACTAACCNNNNNNNNNNNNNNNNNNNNNNNN176530.11700076140301761No Hit
TGATCTCGGAAGCTAAGCAGGGTCNNNNNNNNNNNNNNNNNNNNNNNNNN174210.11546310906939161No Hit
ACCACCCTGAACGCGCCCGATCTCGTNNNNNNNNNNNNNNNNNNNNNNNN173840.11521788003342537No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN172480.11431649763095496No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN171540.1136934833233651No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN171040.11336209273422157No Hit
ACCTGTCCGGTGCCCTGAATGAACTGCAGGNNNNNNNNNNNNNNNNNNNN169360.11224862035469929No Hit
AGAAACCTCCCGTGGAGCAGAAGGGCNNNNNNNNNNNNNNNNNNNNNNNN167830.11123456515192007No Hit
TTGACTCTAGTCTGGCACGGTGAAGAGACNNNNNNNNNNNNNNNNNNNNN167480.11100259173951958No Hit
AGGAGTCTAACACGTGCGCGAGTNNNNNNNNNNNNNNNNNNNNNNNNNNN165560.1097300518772084No Hit
AGCCACCCGAGATTGAGCAATAACNNNNNNNNNNNNNNNNNNNNNNNNNN165510.10969691281829404No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCNNNNNNNNNNNNNNNNNNNN165110.1094318003469792No Hit
AGACGTGGCGACCCGCTGAATTTNNNNNNNNNNNNNNNNNNNNNNNNNNN164820.10923959380527595No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCNNNNNNNNNNNNNNNNNNNNNN163540.10839123389706849No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN163420.10831170015567404No Hit
AGCAGGACGGTGGCCATGGAAGTCNNNNNNNNNNNNNNNNNNNNNNNNNN158410.10499116645245579No Hit
AGTGACGCGCATGAATGGATGAACNNNNNNNNNNNNNNNNNNNNNNNNNN157350.10428861840347148No Hit
AGCAGAACTGGCGCTGCGGGATGAACNNNNNNNNNNNNNNNNNNNNNNNN156290.10358607035448719No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN156160.10349990880130988No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN152730.10122656935978519No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN152520.1010873853123449No Hit
AGACGTGGCGACCCGCTGAATTTAAGCNNNNNNNNNNNNNNNNNNNNNNN152340.10096808470025323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)