FastQCFastQC Report
Sun 2 Jul 2023
SRR3284453.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284453.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19291425
Total Bases964.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14583077.559353443304473No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN10136195.254246381488148No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN3667431.901067443177474No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN3447451.787037505005462No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN3191831.654533037346904No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN2190661.1355615253927587No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN2106221.0917907826923101No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN1879650.974344819006372No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN1310570.6793536506504833No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN1172210.6076326658087726No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN995050.5157991180019101No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN986400.511315260536741No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN819820.4249660146930566No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN816680.423338348514949No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN778610.40360419201795616No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN736850.38195726857917445No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN731360.3791114445926105No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN637520.33046807065833655No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN562980.2918291417041509No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN525110.2721986582121331No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN499790.2590736557822971No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNNN437900.22699204439278073No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN420040.21773404504851246No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN414300.21475862980572974No Hit
CTCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN384730.19943057602017475No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN364030.1887004200052614No Hit
GGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNN362040.18766887360576007No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATGNNNNNNNNNNNNNNNNNNN345920.1793128294047744No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN340660.1765862293739317No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN329010.17054727683413745No Hit
ACCTGTCCGGTGCCCTGAATGAACTGCAGGNNNNNNNNNNNNNNNNNNNN328880.1704798893809037No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN328020.17003409545951115No Hit
CAGTGCGCCCCGGGCGTCGTCGCNNNNNNNNNNNNNNNNNNNNNNNNNNN327060.1695364650356311No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNNNNNNNNNNNNNNNN319000.1653584429351383No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN315990.1637981642102644No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN276030.14308429781625776No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC270900.14042508523864877TruSeq Adapter, Index 6 (100% over 50bp)
AGAACATTCACGATGTACTTGGGACTGAANNNNNNNNNNNNNNNNNNNNN255180.13227638704761313No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN252160.13071092467249049No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNNN250040.1296119908197554No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN246600.12782881513418526No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN244420.12669877937995766No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG242950.12593678279339138TruSeq Adapter, Index 6 (100% over 50bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN231490.1199963196083234No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC219200.11362561345260912TruSeq Adapter, Index 6 (100% over 50bp)
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN218090.11305022827499782No Hit
AGAACATTCACGATGTACTTGGGACTGAACNNNNNNNNNNNNNNNNNNNN211270.10951497880535004No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN209550.10862339096256499No Hit
AAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN209100.10839012670137121No Hit
ACGATGTACTTGGGACTGAACTATGTATTNNNNNNNNNNNNNNNNNNNNN208940.1083071882973912No Hit
CGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNN208130.10788731262724241No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATNNNNNNNNNNNNNNNNNNNN208110.10787694532674492No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNNN205050.10629074835062728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)