FastQCFastQC Report
Sun 2 Jul 2023
SRR3284454.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284454.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19422733
Total Bases971.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14676257.556222906426196No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN10196525.249786422950879No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN3680821.895109200131619No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN3459851.7813404529630306No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN3201991.6485784981959029No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN2201761.1335994785079937No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN2133361.0983830133483274No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN1890270.97322554966904No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN1318930.6790650934654767No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN1175300.6051156652361951No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN1007600.5187735423228029No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN996120.5128629426147185No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN828250.42643329339902886No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN821500.42295798433721965No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN789260.40635887853681557No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN740030.3810122911126874No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN730150.37592546836740226No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN641790.3304323855968158No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN566420.29162734204295554No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN530700.27323652134846316No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN508090.26159552314290685No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNNN440690.22689391858499008No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN420770.2166378953981399No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN416030.21419745614584723No Hit
CTCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN386700.1990965946965342No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN375830.19350005995551708No Hit
GGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNN365180.18801679454688483No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATGNNNNNNNNNNNNNNNNNNN349380.17988199703924262No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN345720.1779976072368394No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN334170.17205096728663263No Hit
ACCTGTCCGGTGCCCTGAATGAACTGCAGGNNNNNNNNNNNNNNNNNNNN330230.17002241651573957No Hit
CAGTGCGCCCCGGGCGTCGTCGCNNNNNNNNNNNNNNNNNNNNNNNNNNN329070.16942517821771014No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN327420.16857565822482345No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNNNNNNNNNNNNNNNN319530.16451340807701986No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN319020.1642508291701276No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN275490.1418389471759716No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC271610.13984128804118348TruSeq Adapter, Index 6 (100% over 50bp)
AGAACATTCACGATGTACTTGGGACTGAANNNNNNNNNNNNNNNNNNNNN259070.13338493609524468No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN253040.13028032666669515No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN250300.1288696086179015No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNNN249750.12858643528693928No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG244840.12605846973234922TruSeq Adapter, Index 6 (100% over 50bp)
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN243900.12557450076670468No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN232090.119493997060043No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC223640.11514342497525966TruSeq Adapter, Index 6 (100% over 50bp)
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN218000.11223961118139243No Hit
AGAACATTCACGATGTACTTGGGACTGAACNNNNNNNNNNNNNNNNNNNN213880.11011838550218447No Hit
ACGATGTACTTGGGACTGAACTATGTATTNNNNNNNNNNNNNNNNNNNNN213080.10970649702078487No Hit
AAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN212460.10938728344770017No Hit
CGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211870.10908351569266796No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN211710.10900113799638805No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATNNNNNNNNNNNNNNNNNNNN210060.10815161800350136No Hit
CGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNNN208270.10723001752636974No Hit
AAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN194730.10025880497868142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)