FastQCFastQC Report
Sun 2 Jul 2023
SRR3284461.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284461.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20613791
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN13088996.349627780741543No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN7435593.6070948812860286No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN5379842.609825625960795No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN5209032.5269636235275694No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN5038232.444106472215615No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN2583331.253204711350765No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNN2574191.248770786508896No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN2467031.1967861709668057No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNN2435921.1816943326921283No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNN2433081.18031661425111No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN2255911.0943692986894065No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN1800570.8734783427269638No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN1664360.8074012198920616No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN1628300.7899080765881442No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNN1480150.7180387149554393No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN1380100.6695032466371663No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN1256400.6094948765125251No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1252180.6074477033360821No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN1249510.6061524539566739No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNN1196470.5804221067342732No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN1170860.5679983851587512No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN1113720.5402790782151619No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN1031560.5004222658510509No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN973090.47205775977839304No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN965800.46852129237169426No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNN940860.4564225959213422No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN913810.4433003128827686No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN911690.44227187517327604No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN895650.4344906766542845No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN856040.4152753852990942No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN842730.40881854288713804No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN819390.39749602584017657No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN806310.39115075921745784No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGATNNNNNNNNNNNNNNNN744790.36130666115708654No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNN653600.3170692862850894No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN619090.3003280667782069No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN579880.2813068202738642No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN570420.2767176595513169No Hit
AGCAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNN564120.2736614531504661No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN557570.27048396871783553No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN545860.2648033057092701No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN532310.25823003638680536No Hit
GCAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNN477460.23162163621431886No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN468570.22730898940422944No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACNNNNNNNNNNNNNN467570.22682387727711023No Hit
AGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNNNNNNNNNN435510.21127118248166968No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNNNNNNNNNNNNNNNN433230.21016512683183797No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGATNNNNNNNNNNNNNNN425330.20633274102759652No Hit
TCGTCGCGCCGTCGGGCCCGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNN410150.19896873893792752No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN392350.1903337430752063No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN391670.19000386682876527No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN386200.1873503034934234No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACANNNNNNNNNNNNNNNNNN384830.18668569987927014No Hit
AGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNNNNNNNNNN367980.17851156053731213No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNN364180.17666813445425927No Hit
GCAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNN363000.17609570214425868No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN351460.17049750819730344No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNNN350790.17017248307213362No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN324730.15753046103940804No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN314000.15232520791541934No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNN307360.14910406339134807No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNNNN300140.14560155383354764No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN296940.1440491950267663No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNNN294900.1430595662874432No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGNNNNNNNNNNNNN289960.14066311237947451No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN280740.13619037856743574No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCNNNNNNNNNNNN276610.13418686548243358No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCANNNNNNNNNNNNNNNNN269620.13079593171387058No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNNN263260.12771061858539268No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGNNNNNNNNNNNNNN260020.12613885529352656No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNNNN258650.1254742516793733No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCNNNNNNNNNNNNNNNNNNNNN255650.12401891529801579No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCNNNNNNNNNNNNNNNNNN255140.123771508113185No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNNN253050.12275762376750593No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNNNN251600.12205421118318313No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNNNNNNNNNNNNNNNNN249170.12087538871428356No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNN247470.12005069809818097No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN239450.11616009883868522No Hit
CGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN234000.11351623774588575No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNN233690.11336585298647882No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNNN229840.11149817129707No Hit
TAAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNN226060.10966444745655955No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNNN225420.10935397569520326No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN223920.10862630750452452No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNN222530.10795200164782888No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAANNNNNNNNNNNNNNNNN221160.1072873980336756No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN220210.10682654151291239No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATNNNNNNNNNNNNNNNN218250.10587572174375884No Hit
AGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNN217840.10567682577163996No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNN208460.10112647401926216No Hit
CGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNNNNN207420.10062195740705822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)