FastQCFastQC Report
Mon 3 Jul 2023
SRR3284462.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284462.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20269158
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN12905646.367131777254882No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN7317623.610223966876177No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN5307922.61871756093667No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN5131492.531673984681554No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN4972872.4534171572395853No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN2556521.2612857426046016No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNN2531061.2487247866931621No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN2438161.2028916050681533No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNN2404911.1864873716017212No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNN2396061.1821211320174227No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN2229871.1001295663095625No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN1767400.8719651798066796No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN1652970.8155099486619031No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN1613000.7957903332738341No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNN1457800.719220798416984No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN1366910.6743792712060364No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1241270.6123934699211482No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN1236820.6101980161188738No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN1229550.606611285974484No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNN1172450.5784404068486713No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN1152910.5688001445348643No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN1087490.536524506839406No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN1006550.4965919156582626No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN964360.47577704017108163No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN958730.47299942109090076No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNN923180.4554604586929561No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN902820.44541564084704455No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN894670.44139475354624996No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN876320.43234159011439943No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN847980.4183597562365442No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN830130.40955327300719646No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN811750.4004853087631958No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN796430.3929270273585118No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGATNNNNNNNNNNNNNNNN737940.36407037726974156No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNN647450.31942619422079593No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN617150.30447737394913No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN572610.2825031015101861No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN566970.2797205488259552No Hit
AGCAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNN559550.2760598146208145No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN552280.2724730844764247No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN539760.26629621220575617No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN522020.257543998620959No Hit
GCAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNN472480.23310292415698766No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN462940.22839626589323545No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACNNNNNNNNNNNNNN457770.22584559259935713No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNNNNNNNNNNNNNNNN428730.21151840643799805No Hit
AGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNNNNNNNNNN422790.2085878456322655No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGATNNNNNNNNNNNNNNN417090.2057756913237343No Hit
TCGTCGCGCCGTCGGGCCCGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNN399240.19696920809438656No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACANNNNNNNNNNNNNNNNNN388740.19178892384182905No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN381880.18840447146349146No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN381160.18804925197188752No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN378220.18659877238117142No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNN363330.1792526359506399No Hit
AGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNNNNNNNNNN362320.17875434194158435No Hit
GCAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNN359620.17742226884806955No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNNN346120.17076190338049563No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN345960.17068296571569475No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN320420.15808254097185487No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN312860.15435273631001348No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNN302870.14942406586400875No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNNNN302680.1493303273870577No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGNNNNNNNNNNNNN289860.1430054469948875No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN289590.14287223968553603No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNNN288350.14226047278332923No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCNNNNNNNNNNNN275820.13607866690861062No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN275310.1358270531020578No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCANNNNNNNNNNNNNNNNN262770.12964031362328912No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNNN261500.1290137459089322No Hit
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGTCCGGNNNNNNNNNNNNNN255150.1258809073371474No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNNNN253740.12518526916608969No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCNNNNNNNNNNNNNNNNNNNNN253330.12498299140003744No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCNNNNNNNNNNNNNNNNNN250170.12342397252022012No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNNN248680.12268886551676197No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCNNNNNNNNNNNNNNNNNNNNNN245670.12120385069769549No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNNNN241280.11903799851972145No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNN240950.11887518958606964No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN237330.1170892249199498No Hit
CGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN229760.11335448665405834No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNNN229700.11332488502975803No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNN228900.11293019670575363No Hit
GACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNNN221800.10942733783021476No Hit
TAAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNN220840.10895371184140949No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN220510.1087909029077577No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN218770.10793245580304817No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAANNNNNNNNNNNNNNNNN217640.10737495854539196No Hit
AGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNN217020.10706907509428858No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNN216660.1068914653484866No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATNNNNNNNNNNNNNNNN215990.10656091387713294No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNN207040.10214533825233392No Hit
CGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNNNNN205720.10149410251772668No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNN205530.10140036404077564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)