FastQCFastQC Report
Sun 2 Jul 2023
SRR3284463.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284463.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21571876
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN314436914.576242696740884No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN7194993.3353566467747173No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN5567302.5808140191423314No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN4950972.295104051219282No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN4647322.154342070202888No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN3158381.4641193005188793No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN2944971.3651895644124785No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN2853041.3225738920435108No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN2351261.0899654717095537No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN2090550.9691090380827333No Hit
AGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNN1837100.8516180975636982No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN1705680.7906961823811707No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN1568130.7269326042853204No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN1432550.6640822522807011No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNN1365450.6329769371935942No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN1310570.6075364052713821No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN1264500.5861798945998021No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN1111310.5151661357593563No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN1104050.511800642651571No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN983320.45583425382196713No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN966920.4482317625041049No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN933570.43277181826930583No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNN911340.4224667340012524No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN864920.40094797503935214No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN854700.3962103249620015No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN844240.39136141891414544No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNN784250.363552061953258No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNN770030.35696014570081896No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN725250.336201635870705No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN710050.3291554244053693No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNNN703600.32616542019803935No Hit
AACCCGGCGGCGCGTCCGGCCGTGCCGGNNNNNNNNNNNNNNNNNNNNNN697830.3234906412404744No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNNN661720.30675125334486436No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNNN654460.303385760237079No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN645550.2992553823320698No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN625990.2901880207358878No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN597230.2768558469370026No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN581200.2694248752403361No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN554790.25718208281931526No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN539870.2502656699862358No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNN524970.2433585284840317No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACANNNNNNNNNNNNNNNNNN504410.23382760034407762No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN488870.22662377625385943No Hit
CTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNN488330.2263734503202225No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN454690.21077907178772953No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN452080.2095691631084844No Hit
AGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNNNNNNNNNN450610.20888772028913946No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN448300.20781688157302589No Hit
AGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNNNNNNNNNN442960.20534143622928297No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNN418610.1940535908884327No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNNN404510.1875173026212463No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNN396860.18397101856138984No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN355530.16481181330728956No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNNNNNNNNNNNNNNNN354750.16445023140314732No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNN338440.1568894610742246No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNN335150.15536432714521445No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN333810.15474314797655986No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN316370.1466585474531747No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN300420.1392646610800099No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN292600.1356395707077122No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNNN277460.12862117323500283No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGNNNNNNNNNNNNNNNNN267950.1242126554037303No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN264790.12274778512541053No Hit
AAGGAGAGGGTATCAAGCACATTATNNNNNNNNNNNNNNNNNNNNNNNNN256550.11892799680472853No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNNNN255950.11864985687846528No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCNNNNNNNNNNNNNNNNNNNNN254250.11786179375405273No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNN252690.11713862994576828No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN252370.11699028865176121No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN242010.11218773925828239No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNN240750.11160364541312959No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNN237510.11010168981130802No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNNN237370.11003679049517993No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNNNN234510.10871099017999176No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN233830.10839576493022675No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGATNNNNNNNNNNNNNNNN232020.10755670948599927No Hit
TAAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNN229860.10655540575145157No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN219980.10197536829898338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)