FastQCFastQC Report
Sun 2 Jul 2023
SRR3284464.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284464.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21275904
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN311571614.644341316824894No Hit
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN7100223.337211899433274No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN5498062.5841722166071066No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN4904872.305363851989556No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN4615602.1694025316151078No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN3137931.4748750511376627No Hit
AGCCCGTGGACGGTGTGAGGCCGGTNNNNNNNNNNNNNNNNNNNNNNNNN2914191.3697138321361104No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN2814791.3229943131911104No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN2322381.091554088606529No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN2069270.9725885207979882No Hit
AGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNN1807240.8494304166817072No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN1689720.794194220842508No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN1559170.7328337258900961No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN1413380.664310198053159No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNN1350560.6347838380921441No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN1288980.6058402970797387No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN1252520.5887035399294902No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN1100700.5173458199472981No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN1082020.5085659344956623No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN968550.45523330054506733No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN960020.45122407019697025No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN920870.43282297194046376No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNN903690.4247481094105331No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN863310.4057688923582284No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN845360.39733211806182245No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNN827950.3891491520172304No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNN773300.3634628169031031No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNN763670.3589365697457556No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN718390.33765427781588037No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN705920.3317931872601042No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNNN695430.32686272696097896No Hit
AACCCGGCGGCGCGTCCGGCCGTGCCGGNNNNNNNNNNNNNNNNNNNNNN686050.3224539836239156No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNNN653840.3073147914184986No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNNN650640.3058107425188608No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN636270.29905662292892465No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN613700.28844837803366663No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN587220.2760023733891636No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN576240.2708416056022814No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN550830.25889851730859476No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN529290.24877438815290764No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNN520650.2447134561238855No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACANNNNNNNNNNNNNNNNNN497370.2337715003790203No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN483430.22721948735997302No Hit
CTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNN480320.22575773983563754No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN452770.21280881884031813No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNNN447600.21037883983684078No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN445620.2094482095801899No Hit
AGATCAGACGTGGCGACCCGCTGAANNNNNNNNNNNNNNNNNNNNNNNNN442720.2080851652648931No Hit
AGATCAGACGTGGCGACCCGCTGANNNNNNNNNNNNNNNNNNNNNNNNNN435900.20487966104753996No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNN413560.19437951966694342No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNN398110.18711778357337955No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNNN397880.18700968005871807No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGNNNNNNNNNNNNNNNNNNNNN354670.16670031976079605No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN353210.1660140974503363No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNN336240.15803793812944447No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN331940.15601687242055615No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCNNNNNNNNNNNNNNNN331190.15566436095970354No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN314630.1478809079040778No Hit
AGGGCTTCTGTCGTGAGTGGCACACGTAGGNNNNNNNNNNNNNNNNNNNN297670.13990944873599728No Hit
AAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNNN289120.1358908180822775No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGCNNNNNNNNNNNNNNNNNN272910.12827187037504964No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN266430.12522617135328304No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGAGGNNNNNNNNNNNNNNNNN264160.12415923666510245No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCCCGANNNNNNNNNNNNNNNNNNN253510.1191535739209953No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCNNNNNNNNNNNNNNNNNNNNN253090.11895616750291786No Hit
AAGGAGAGGGTATCAAGCACATTATNNNNNNNNNNNNNNNNNNNNNNNNN252870.11885276414106775No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN249020.117043205308691No Hit
CAGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGNNNNNNNNNNNNNNNNN247920.11652618849944049No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN241180.11335828550457833No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNN238920.11229605096920911No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNNN235470.11067449824928709No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNN234660.11029378587156626No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN230440.10831032138516887No Hit
AGCTCGCCGAATCCCGGGGCCGAGGGAGCCCGATNNNNNNNNNNNNNNNN229990.10809881450865731No Hit
GTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACNNNNNNNNNNNNNNNNN229910.10806121328616636No Hit
TAAACTCTAAGGAGAGGGTATCAAGCNNNNNNNNNNNNNNNNNNNNNNNN229870.1080424126749209No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN218510.1027030390812066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)