FastQCFastQC Report
Sun 2 Jul 2023
SRR3284465.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284465.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17349782
Total Bases867.4 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN8979905.175799903422417No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN7492884.318717088203183No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN4993892.87835893269437No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN2532131.459459260064478No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN2255701.3001316097228195No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN2142331.2347878492075577No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN1777591.0245604238716084No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN1720780.991816496599208No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN1665420.9599083146981328No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN1656430.9547266933959171No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN1464910.8443391392468217No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN1459510.8412267082087832No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN1458640.8407252609859882No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN1390230.8012953707429868No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1097900.6328033401226597No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN1011710.583125482498858No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN948300.5465774728466328No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN875100.5043867409976679No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN817420.47114136650247246No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN663160.3822295865158421No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN625260.36038493163775775No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN605750.34913983357254863No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN587560.33865555198330444No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN562170.32402136234334245No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN508570.2931276024102205No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN502910.2898653135814617No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATGNNNNNNNNNNNNNNNNNNN451180.26004937698929015No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN445180.2565911202803586No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN371940.21437733338666734No Hit
TCGTCGCGCCGTCGGGCCCGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNN340740.19639439850022322No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN340490.19625030447068442No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN328490.1893337910528213No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN324520.18704557786374493No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN317480.18298788999193188No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN311340.1794489406264586No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN310640.1790454773437499No Hit
TGCGAGAGGTCCCGGGTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300830.17339122762464682No Hit
AACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN300710.17332206249046817No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN292850.16879174620176784No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN274480.15820371691125573No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN267080.1539385336369068No Hit
GGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNN265010.1527454350723254No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNNN264170.152261279133075No Hit
CGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN230470.13283740395124274No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATNNNNNNNNNNNNNNNNNNNN219350.12642810151735623No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN215090.12397273925401484No Hit
GTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNN214930.12388051907511No Hit
GTGGTCTAGTGGTTAGGATTCGGCNNNNNNNNNNNNNNNNNNNNNNNNNN209990.12103322105142302No Hit
ACCTGTCCGGTGCCCTGAATGAACTGCAGGNNNNNNNNNNNNNNNNNNNN208790.12034156970963669No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN205870.11865855144462334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNNNNNNNNNNNNNNNN203580.1173386501340478No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN203560.11732712261168468No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN193180.11134433850523309No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNN192130.11073914358117007No Hit
AGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNN190960.11006478352292841No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN187200.10789760931866466No Hit
AACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNNNNNNNNNNNNNNNN186520.10750567355831905No Hit
AGTACTTCCAGGAACGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178420.10283702700126145No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNN174580.10062374270754526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)