FastQCFastQC Report
Sun 2 Jul 2023
SRR3284466.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3284466.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17475360
Total Bases873.7 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNN9071165.190828686791002No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNN7532254.310211635125113No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN5019592.8723814559471164No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNN2558261.4639240622224663No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNN2272431.3003623387443808No Hit
AGTGCGCCCCGGGCGTCGTCGCGCNNNNNNNNNNNNNNNNNNNNNNNNNN2164831.2387899305078693No Hit
GTCTAGCGGTTAGGATTCCTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNN1789891.024236410580383No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNN1746060.9991553822067185No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNN1680860.9618457073273455No Hit
GTCTAGGGGTATGATTCTCGCTTNNNNNNNNNNNNNNNNNNNNNNNNNNN1659200.9494511128812225No Hit
ATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNNNNNNN1487590.8512499885553144No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNN1470860.841676509096236No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTNNNNNNNNNNNNNNNNNNNNNNN1467600.8398110253522674No Hit
TCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN1408470.8059748125360507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1100340.6296522646743759No Hit
TCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNN1018460.582797722049789No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNN958040.548223327015867No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNN888570.5084702117724613No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNNN828690.47420482324827645No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNN673760.38554856666758225No Hit
CGACTGCATAATTTGTGGTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNNN628920.35988958167385393No Hit
AGGATCGTTTCGCATGATTGAACAAGATGGNNNNNNNNNNNNNNNNNNNN613950.3513232345428077No Hit
GCTGGTCCGATGGTAGTGAGTTATCTCAANNNNNNNNNNNNNNNNNNNNN586900.3358442973420862No Hit
GCGCAGCGGAAGCGTGCTGGGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN565340.3235069263236923No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNN510210.29195965061663964No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNNN509010.2912729694838905No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATGNNNNNNNNNNNNNNNNNNN449160.25702474798802427No Hit
TCGACTGCATAATTTGTGTTAGTGGGGGNNNNNNNNNNNNNNNNNNNNNN447220.25591461349007977No Hit
TCGACTGCATAATTTGTGTTAGTGGGGNNNNNNNNNNNNNNNNNNNNNNN377360.21593832687853068No Hit
TCGTCGCGCCGTCGGGCCCGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNN342080.1957499015757043No Hit
CGCCCCTCCCACGGCGCGACCGCTCTNNNNNNNNNNNNNNNNNNNNNNNN340280.19471987987658051No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGNNNNNNNNNNNNNNNNNNNNNN331000.18940954578332006No Hit
ATCGTATAGTGGTTAGTACTCTGCGTNNNNNNNNNNNNNNNNNNNNNNNN323660.18520934618800414No Hit
CGCCCCTCCCACGGCGCGACCGCTCNNNNNNNNNNNNNNNNNNNNNNNNN318430.18221656091777222No Hit
GGTGGGGGCCGGGCCGCCCCTCCCACGGCGCNNNNNNNNNNNNNNNNNNN314420.1799219014658353No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNN311520.17826242206169143No Hit
AACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN301550.17255724631710018No Hit
TGCGAGAGGTCCCGGGTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN299220.17122394045101216No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGNNNNNNNNNNNNNNNNNNNNN299130.17117243936605597No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGNNNNNNNNNNNNNNNNNN276200.15805110738777342No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGNNNNNNNNNNNNNNNNN271920.15560194468096794No Hit
CGACTGCATAATTTGTGGTAGTGGGGGANNNNNNNNNNNNNNNNNNNNNN268320.15354190128272036No Hit
GGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNNN266920.15274077329451297No Hit
CGTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNN234810.13436633065069906No Hit
TCAAGAGACAGGATGAGGATCGTTTCGCATNNNNNNNNNNNNNNNNNNNN225000.12875271239047437No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCNNNNNNNNNNNNNNNNNNN216490.12388299869072797No Hit
GTATAGTGGTTAGTACTCTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNN215940.12356826983821792No Hit
GTGGTCTAGTGGTTAGGATTCGGCNNNNNNNNNNNNNNNNNNNNNNNNNN212330.12150250409719743No Hit
ACCTGTCCGGTGCCCTGAATGAACTGCAGGNNNNNNNNNNNNNNNNNNNN209390.1198201353219619No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGANNNNNNNNNNNNNNNNN208910.11954546286886221No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCNNNNNNNNNNNNNNNNNNNN204430.11698185330659855No Hit
TGGCGCGGCGCGGGAAATGTGGCGTACGGAAGNNNNNNNNNNNNNNNNNN203620.11651834354199284No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGNNNNNNNNNNNNNNNNNNNN195930.11211786194962507No Hit
AGATAACCTCGGGCCGATCGCACNNNNNNNNNNNNNNNNNNNNNNNNNNN194980.11157423938619863No Hit
CGGTGGGGGCCGGGCCGCCCCTCCCNNNNNNNNNNNNNNNNNNNNNNNNN192020.10988042592541727No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATNNNNNNNNNNNNNNNN188360.10778604847053222No Hit
AACGATTAAAGTCCTACGTGATCTGAGTNNNNNNNNNNNNNNNNNNNNNN188040.1076029335017991No Hit
AGTACTTCCAGGAACGGTGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178830.10233265580794901No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCNNNNNNNNNNNNNNNNNNNNNNN177130.10135985753655433No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)