FastQCFastQC Report
Sun 2 Jul 2023
SRR3286543.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3286543.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110777954
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA54998674.964766726058147Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG40602203.6651877502630175Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA21671611.9563107294796218Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT21602411.9500639991960853Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT16824091.5187218568777683Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTAGATCGGAAGAGCACACGTCTG13999781.2637695041740886Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA13135021.1857070405904049Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA12015601.084656248480632Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA11141031.0057082296356548Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGA10272060.9272657265361661Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG9457600.8537438775949951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA8652610.7810768918877127Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTG7207750.6506484133115511Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTC7122310.6429356873660981TruSeq Adapter, Index 5 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT6493870.5862059882420287Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA5921500.5345377655196629Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGAA5890500.5317393747857088Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA5383510.485973048391921Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTAGATCGGAAGAGCACACGTCTG4867390.4393825507916494Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA4756270.42935167407045627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4645560.4193578083234864No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG4583000.41371047528102933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC4274060.3858222548504552Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAGCACACGTCTGA4037770.36449219851090586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTGA3731400.33683597369924345Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCGGAGATCGGAAGAGCACACGTCT3596090.3246214494988777Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT3588440.3239308788822729No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTCAGATCGGAAGAGCACACGTCTG3266470.29486643163674964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCAAGATCGGAAGAGCACACGTCTGA2880070.2599858451980437Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC2848100.2570998919153174No Hit
GCATTGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGAA2744550.24775236415722213Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCAGATCGGAAGAGCACACGTCTGAA2715650.24514354182782616Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG2697630.24351686437537923TruSeq Adapter, Index 5 (100% over 49bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTC2588920.23370353996608387TruSeq Adapter, Index 5 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2547550.22996904239628763No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT2467550.22274738888930912Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2438340.22011058265257363No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2365120.2135009642803116No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2341280.21134891153523203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGAGATCGGAAGAGCACACGTCTG2328860.21022774982827358Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTGAGATCGGAAGAGCACACGTCTG2307330.208284222328208Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG2286090.20636687332210524No Hit
GCATTGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGAA2114600.1908863563232085Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAAGATCGGAAGAGCACACGTCT1978420.17859329664095439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGAGATCGGAAGAGCACACGTCTGA1902430.1717336285160132Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1857660.16769221067217038No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG1844160.16647355664286778Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATCAGATCGGAAGAGCACACGTCTGAA1818920.16419512496141606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTC1810110.16339984036896008No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTC1757210.15862452198747054No Hit
GCCGTGATCGTATAGTGGTTAGTAAGATCGGAAGAGCACACGTCTGAACT1748680.15785451318228896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1619150.14616175344780244Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATGAGATCGGAAGAGCACACGTCTGAA1579650.14259606202873182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACCAGATCGGAAGAGCACACGTCTGAA1524380.13760680216209806Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1522850.1374686880387771No Hit
GCCGTGATCGTATAGTGGTTAGTACTCCGAGATCGGAAGAGCACACGTCT1504290.1357932644251581Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACGAGATCGGAAGAGCACACGTCTGAA1496090.1350530449406928Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG1419030.1280967872000958Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCTG1402430.12659829409739776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA1400500.1264240717065419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACAGATCGGAAGAGCACACGTCTGAAC1384200.12495265980449503Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCTCGTTGGTCTAGGGGTATGATTCTAGATCGGAAGAGCACACGTCTGA1346580.1215566772428384Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAAC1341100.12106199397761037Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA1308700.11813722430728409Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTAGATCGGAAGAGCACACGTCTGAACTC1265270.11421676915968317Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTAA1264800.11417434194532967Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCG1240030.11193833747823147TruSeq Adapter, Index 5 (100% over 45bp)
GCCGTGATCGTATAGTGGTTAGTACTCCAGATCGGAAGAGCACACGTCTG1211180.10933402868227733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1205460.1088176804565284No Hit
GTGGTTCGTGCTGAAGGCCTGTATCCTAGGCTACACACTGAGGACTAGAT1174900.1060590088168626No Hit
GTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1171640.10576472643645324Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCATTTGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA1162940.10497937161756932Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCGAGATCGGAAGAGCACACGTCTG1131380.10213042930906631Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTCAGATCGGAAGAGCACACGTCTG1131160.10211056976192213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)