FastQCFastQC Report
Sun 2 Jul 2023
SRR3286544.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3286544.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95891656
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA32946983.435854731719306Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG27992902.9192216682544307Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT19463222.02970944625255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA14289041.490123395094981Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA11135311.161238679619841Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT8920620.9302811498009796Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA8449310.8811308879679791Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA7969110.831053538172289Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT7572220.7896641184296578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA7570540.789488920704425Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGA6462830.6739720920035003Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT6198220.6463774074357419No Hit
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA6168050.6432311482867706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTAGATCGGAAGAGCACACGTCTG5779490.6027104172650851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA5059410.5276173351308063Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT5030140.524564931906067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG4952240.5164411802420015Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTG4939470.5151094689615121Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC4704670.49062350117303216No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG4686930.4887734966220627No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4403660.4592328658918978No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGAGATCGGAAGAGCACACGTCTG4396450.4584809756544407Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT4338800.4524689822855911No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG4119230.42957126530383416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGAA3731970.3891861039504835Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG3675100.3832554523826348No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTAGATCGGAAGAGCACACGTCTG3349790.34933070714724124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAGCACACGTCTGA3184680.3321123164251121Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC3180970.3317254214485565No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2897850.30220043337243024No Hit
TCCCTGTGGTCTAGTGGCTAGGATTCGAGATCGGAAGAGCACACGTCTGA2816350.2937012580114374Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA2688880.2804081306094036Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG2688360.28035390274206967No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTGA2647930.2761376860568557Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC2510290.2617839867110022Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCGGAGATCGGAAGAGCACACGTCT2363110.24643541456829152Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2233510.23292016147890907TruSeq Adapter, Index 6 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2202950.22973323142943738No Hit
GCATGGGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGA2194420.22884368583644027Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2077440.21664450137350846No Hit
GCATTGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGAA2062510.21508753587486276Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1969290.20536614781165108No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1912100.19940212524852005Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC1904200.19857827880248516TruSeq Adapter, Index 6 (100% over 50bp)
GCATTGGTGGTTCAGTGGTAGAATTCAAGATCGGAAGAGCACACGTCTGA1903370.19849172278347138Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCAGATCGGAAGAGCACACGTCTGAA1900330.19817469832828835Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAAGATCGGAAGAGCACACGTCT1819860.1897829358583608Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1773910.18499106950452499No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1741490.18161017054497422No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC1684980.17571706134681833No Hit
GCATTGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGAA1640010.17102739366603492Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACGAGATCGGAAGAGCACACGTCTGAA1617310.16866013868818783Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACCAGATCGGAAGAGCACACGTCTGAA1578990.16466396200311734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1463680.15263893242181573No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC1369940.1428633164912701No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTCAGATCGGAAGAGCACACGTCTG1354800.14128445127697034Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCTG1327550.13844270245995127Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACAGATCGGAAGAGCACACGTCTGAAC1284780.13398246037173453Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT1272790.13273209089224614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCAAGATCGGAAGAGCACACGTCT1271360.1325829642570778Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG1227440.12800279515456484No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC1207160.12588790832854113No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGAGATCGGAAGAGCACACGTCT1155350.12048493562359587Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1129890.11782985581143786No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1117590.11654715818026963No Hit
GCCGTGATCGTATAGTGGTTAGTACTCCAGATCGGAAGAGCACACGTCTG1103620.11509030566747122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCGAGATCGGAAGAGCACACGTCTG1091880.1138660072780472Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1060960.11064153485888283Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATCAGATCGGAAGAGCACACGTCTGAA1030700.10748589011748842Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA1003260.10462432727202041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAGATCGGAAGAGCAC1001010.10438968746144085No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT988010.1030339907780923No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG984080.10262415324228001No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGGAGATCGGAAGAGCACACG960610.1001765993070346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)