FastQCFastQC Report
Sun 2 Jul 2023
SRR3286545.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3286545.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111526324
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG31613242.834598941860578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA30226032.7102148547458627Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTAGATCGGAAGAGCACACGTCTG20029431.7959374326728461Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA13977631.2533032111773001Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC13823181.2394544627867408TruSeq Adapter, Index 7 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGA11677191.0470344203221473Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT10414850.9338467929777726Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA10044540.9006429728644153Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCT9320400.8357130106789855Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGAGATCGGAAGAGCACACGTCTG9173690.8225582688442238Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA8588960.7701284945068215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC8228840.7378383600270013TruSeq Adapter, Index 7 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA8187000.7340867793687883Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG7565250.678337609334277TruSeq Adapter, Index 7 (100% over 49bp)
GCATTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGAA6995240.6272277027619058Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCG6476300.5806969841487827TruSeq Adapter, Index 7 (100% over 45bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTG6414720.5751754177784969Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGAGATCGGAAGAGCACACGTCTGA5856000.5250778282623212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTTCTGTAGTGTAGTGGTTATCACGTGAGATCGGAAGAGCACACGTCTG5845180.5241076537230798Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTCTGTAGTGTAGTGGTTATCACGTCAGATCGGAAGAGCACACGTCTG5600410.5021603688829553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTAGATCGGAAGAGCACACGTCTG4887460.4382337572607522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG4736220.4246728332944965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA4185960.37533380908349495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGA3941410.35340625052790225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCAAGATCGGAAGAGCACACGTCTGA3762030.33732215544018107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGAA3254600.2918234801677853Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGA2869660.2573078621330691Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTGA2824280.2532388676237549Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2762010.24765543245198327No Hit
GCATTGTGGTTCAGTGGTAGAATTCGAGATCGGAAGAGCACACGTCTGAA2746040.24622348352484028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2658960.23841546144746958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAGATCGGAAGAGCACACGTCT2642800.23696647618368558Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2570220.2304585955868141No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG2488540.22313476412976724No Hit
GACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC2471950.22164722294621672TruSeq Adapter, Index 7 (100% over 44bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT2369380.21245029110795402No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2323140.20830418475910675No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTC2288130.20516501557067368No Hit
GACGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCT2236290.20051678561556463TruSeq Adapter, Index 7 (100% over 43bp)
GAGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTC2166700.19427700315846508TruSeq Adapter, Index 7 (100% over 44bp)
TCCTCGTTAGTATAGTGGTGAGTATCAGATCGGAAGAGCACACGTCTGAA2146430.1924594950336568Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2135320.1914633176648053No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG2109260.18912664959709422No Hit
GCATTGTGGTTCAGTGGTAGAATTCCAGATCGGAAGAGCACACGTCTGAA1957910.17555586248857263Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCGGAGATCGGAAGAGCACACGTCT1941180.17405576821486557Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGAGATCGGAAGAGCACACGTCT1940900.17403066203455247Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCAAGATCGGAAGAGCACACGTCTG1940250.17397237983025424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA1895940.1699993267957079Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTAGATCGGAAGAGCACACGTCTG1841300.16510003503746792Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1801430.1615250942907434No Hit
GCATTGGTGGTTCAGTGGTAGAATTCAGATCGGAAGAGCACACGTCTGAA1782750.1598501534041416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC1772270.15891046494099456Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGAACTTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAT1747580.15669663782695822TruSeq Adapter, Index 7 (100% over 38bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT1699610.15239541114974794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTTGTGGTTCAGTGGTAGAATTCTAGATCGGAAGAGCACACGTCTGA1590990.14265600648686314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1577120.14141235391206833No Hit
TCCCTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAAC1557310.13963609165491725Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1506070.13504166065762196No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACG1476820.13241896146420104No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1405860.12605633805342675No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTCAGATCGGAAGAGCACACGTCTG1378730.12362372851094777Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCACGTGAGATCGGAAGAGCACACGTCTG1368000.12266162381537835Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGCTAGGATTCGGAGATCGGAAGAGCACACGTCTG1359620.12191023170457944Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1346430.12072755128197357No Hit
TCCCTGGTGGTCTAGTGGTTAGGATGAGATCGGAAGAGCACACGTCTGAA1324400.11875223288091159Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCAAGATCGGAAGAGCACACGTCTGA1265840.11350145459828838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGCTAGGATTCAAGATCGGAAGAGCACACGTCTGA1255300.11255638623935994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCAGATCGGAAGAGCACACGTCTGA1231750.11044477714516979Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGGAGATCGGAAGAGCACACG1216850.10910876969279469No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTAAGATCGGAAGAGCACACGTCTG1214790.10892405993763409Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTTAGATCGGAAGAGCACACGTCTGA1190210.10672009596586363Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTAT1173450.10521731174426587TruSeq Adapter, Index 7 (100% over 48bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1171730.10506308806519973No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTAAGATCGGAAGAGCACACGTCT1142260.10242066258724712Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATCAGATCGGAAGAGCACACGTCTGAA1135730.101835150596374Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)