FastQCFastQC Report
Sun 2 Jul 2023
SRR3296795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24253357
Total Bases524.6 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAATCAGTTATCGTTTATTTGATAGTT2947171.2151596168728314No Hit
ATGGAGAACAGAAATCTCCAGT2666491.0994313075917697No Hit
TTTAGGGATAACAGGGTAAT2653641.094133071970202No Hit
TCACGTTCTAGCATTCAAGGTCCCA2282360.9410491092016663No Hit
TTTGCTCGAATATATTAGC2087420.8606726070951745No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC2040400.8412856001748542No Hit
TTGCTCGAATATATTAGC2005300.8268133768038791No Hit
AGTTATCGTTTATTTGATAGTT1999730.8245167875111061No Hit
ATTGTAATTATTGCTCTTC1910690.7878043439512311No Hit
TTTAACGATCAACTTAGAACTGGT1708170.7043025012990985No Hit
TCACGTTCTAGCATTCAAGGTCCC1499140.6181164941414089No Hit
CTACATGGTATAACTGTGGT1495430.6165868089930808No Hit
ATGTCTAAGTATAAGCAAT1380380.5691500768326627No Hit
AGTCAAGGATGCTGGC1371670.565558821403569No Hit
AAATATAGATGGATACGAAT1275230.5257952538281607No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC1245730.5136319891716433No Hit
AAAATCAGTTATCGTTTATTTGATAGTT1226910.5058722386348413No Hit
AAAAGCGAATGATTAGAGGT1206970.49765069635514786No Hit
ACGATCAACTTAGAACTGGT1159780.47819359604528144No Hit
AAATTCTAGAGCTAATACATGCT1143240.47137392155650865No Hit
AACGGGGTATTGTAAGC1126600.46451301566212044No Hit
AAATATAGATGGATACGAATAAGGCGTC1055750.43530056478367096No Hit
GTTTTCATGCCAGGTCGT1014130.4181400537665776No Hit
AGACACCACAAAAGGTGTTAGTTC969920.3999116493440475No Hit
TGGAGAACAGAAATCTCCAGT903390.37248039518817955No Hit
AAGCGGAGGAAAAGAAACC890770.367276991799527No Hit
CACGTTCTAGCATTCAAGGTCCCA877150.3616612743547213No Hit
AGGGGATCGAAGATGATC849840.3504009774811792No Hit
TCTACATGGTATAACTGTGGT842500.3473745923090152No Hit
ATCGTTTATTTGATAGTT824670.3400230326878048No Hit
ATGTCTAAGTATAAGCAA797530.3288328292038088No Hit
TTTTACTTTGAAAAAATTAGAGTGTTC789720.32561265642525283No Hit
ATATAGATGGATACGAAT776200.3200381703860624No Hit
AGAAAGTGATGTTGACGC759510.3131566487888666No Hit
TTGGAATGAGTACAATGTAAATACCTT737160.30394142963384413No Hit
AAGAGGTGCACAATCGACCGATCCTGA704030.2902814649534908No Hit
TATAGATGGATACGAATAAGGCGTC695000.28655826902642795No Hit
AAGGAGTGTGTAACAACTC691820.285247110327861No Hit
AACGATCAACTTAGAACTGGT684520.28223721771794313No Hit
AAAAGCGAATGATTAGAGGTT683940.2819980755653743No Hit
ATTGCTCGAATATATTAGC682550.28142495902732145No Hit
AGATGGATACGAATAAGGCGTC669950.27622980191979196No Hit
TTGGAATGAGTACAATGT658540.2715252985390847No Hit
ATTAATCAAGAACGAAAGTT644800.2658601034075407No Hit
AGAAATCTCCAGTAGAAC642110.2647509785964887No Hit
ACAATCGACCGATCCTGA639700.26375730172115963No Hit
CCACGTTCTAGCATTCAAGGTCCCA627290.2586404842842993No Hit
ATATAGATGGATACGAATAAGGCGTC624700.25757259087886264No Hit
AGAAGCTCGTATCAGTTTT620980.256038782589973No Hit
ATGGATTTGAGTAAGAGC604870.249396403145346No Hit
ATTGTAAGCAGTAGAGT603740.24893048826189296No Hit
ACGTTCTAGCATTCAAGGTCCCA598260.24667100723417382No Hit
CACGTTCTAGCATTCAAGGTCCC598020.24657205186069706No Hit
ATCCTGTCTGTGACGCCA595050.24534747911392224No Hit
ATTAGCATGGAATAATAGAAT584980.24119547656846022No Hit
TTTGACCTCAAATCAGGT584370.24094396499420678No Hit
AAGAAGTCCTTGTTACTT577320.2380371508983272No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA572900.23621472277013034No Hit
AGTTTTATGAGGTAAAGC562130.2317741003853611No Hit
ACTTTGAAAAAATTAGAGTGTTC542460.22366388290082895No Hit
AAGAGGTGCACAATCGACCGATCCTG542000.2234742184349985No Hit
ATAGCATTGCGATGGTC537240.2215116035277096No Hit
ATTTGAATGAAGAGCTTTT516330.21289011661354754No Hit
TTAACGATCAACTTAGAACTGGT516050.21277466867782469No Hit
TCCCGCTAGAGACCACCA511030.21070485211593595No Hit
ATGATGCCCTGACGAGT495270.20420678259096256No Hit
AAATGTGAATTGCAGAATT490180.2021081040451431No Hit
ACCCGCTGAACTTAAGC470640.1940514873879109No Hit
AGCATGGAATAATAGAAT464740.19161883445660738No Hit
ATGCATGTCTAAGTATAAGCAA461570.19031179889860195No Hit
AAATAAGCGGAGGAAAAGAAACC454100.18723181289913804No Hit
CCCTCACTGGGGTCGCCA452260.18647315503581627No Hit
ATGCATGTCTAAGTATAAGCAAT447510.1845146632690889No Hit
ACTAAATAGTGGTGCTAGC446100.18393330044991296No Hit
TGACTTTTACTTTGAAAAAATTAGAGTGTTC443600.18290251530953014No Hit
TATGATGCCCTGACGAGT428120.17651989372027962No Hit
TTGGGCCAGCATCAGTTTTGGTGGC423370.17456140195355224No Hit
AGAATAGGACGTTTGGTTCTA421620.17383985235528426No Hit
CCACGTTCTAGCATTCAAGGTCCC420810.17350587796980022No Hit
AAATATAGATGGATACGAATAAGGCGTCC419420.17293276143174738No Hit
AGTCAAGGATGCTGGCAT406940.16778708201095627No Hit
TTGGAATGAGTACAATGTAAAT404700.16686349852517324No Hit
ACGGGGAAACTCACC401350.16548224643706022No Hit
TATATAGATGGATACGAATAAGGCGTC397870.16404739352164735No Hit
ATAAATCATTTGTATACGACTT396840.1636227100438096No Hit
TTTAACGATCAACTTAGAACT395290.16298362325677224No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC395140.1629217761483493No Hit
ACGTTCTAGCATTCAAGGTCCC392310.16175492736943592No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC386760.15946658435778602No Hit
AAATAGAATAGGACGTTTGGTTCTA380980.1570834091132209No Hit
AACGAGGAATTCCTAGT380790.1570050694425518No Hit
ACTGATAACCGCAGC378890.15622167273586085No Hit
AAGGACGTTTTCATTAATC372040.15339732145121188No Hit
TTGACCTCAAATCAGGT371820.1533066123588582No Hit
ACAATCGACCGATCCTG365290.15061420157217825No Hit
ATCTAATTAGTGACGCGC364840.15042866024690935No Hit
ATCCGGGTAGGACCTCCA363600.14991739081727945No Hit
TTTGACCTCAAATC358470.14780221970921387No Hit
AAGCGTGTTACCTATACTCT357420.14736928995025309No Hit
ATCATACCGAAGCAGAATTCGGT355750.14668072547647734No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA354530.14617770232797053No Hit
AAGTCAAGGATGCTGGC353890.1459138213320325No Hit
ACTTAATTGAACGTGGAC352470.14532833537229506No Hit
ATGGCTGGAAGAGGCCAGCA350230.14440475188651203No Hit
AGTGAAATACCACTACCTTT344160.14190200556566251No Hit
ATCCGATTGGAAGCACCA342370.14116396340514842No Hit
TTATAGATGGATACGAATAAGGCGTC337370.13910239312438274No Hit
ATGCATGTCTAAGTATAAGC336700.13882614270676014No Hit
AATAAGCGGAGGAAAAGAAACC332060.1369130054862096No Hit
TCACGTTCTAGCATTCAAGGTCC331920.13685528151834817No Hit
ATAATAACTTTTCGAATCGC326890.1347813418158979No Hit
TGCTCGAATATATTAGC324380.13374643353495352No Hit
AGAATGGGCGCTTGTCGCGTGCC319740.131833296314403No Hit
AAGCCTTTGTTGTCTGATTTGT318720.1314127359771268No Hit
TAGTTATCGTTTATTTGATAGTT317410.1308726045635662No Hit
TAACGATCAACTTAGAACTGGT316470.13048502935078224No Hit
AGTGACAATAAATAACG313560.12928519544737663No Hit
ATCAGACACCACAAAAGGTGTT301980.12451059867712333No Hit
AGTGGGCCATTTTTGGT300860.12404880693423183No Hit
TACATGGTATAACTGTGGT300600.12394160527963202No Hit
AAATGTGAATTGCAGAATTC296730.12234594988231938No Hit
ACCGTCAGGGTTGATATGATGCCCTGACGAGT295760.12194600524785085No Hit
TCAATATAGATGGATACGAAT292120.12044518208345342No Hit
CTGTTTGAGCGTCATTT291990.12039158125615353No Hit
AGAGGTGAAATTCTTGGATTT290140.11962880025227023No Hit
ACAATATAGATGGATACGAAT288900.11911753082264034No Hit
ACTTCTTAGAGGGACT286790.11824754816415724No Hit
ATATTAGCATGGAATAATAGAAT285680.11778987956182725No Hit
TAGATGGATACGAATAAGGCGTC285220.1176002150959968No Hit
AAGTGGGTGGTAAATTCC282270.11638388863034507No Hit
TAAGGACGTTTTCATTAATC280570.11568295473488475No Hit
ACTGATGAATGTTACCGC277260.11431819520901787No Hit
CAAGAGGTGCACAATCGACCGATCCTGA274700.11326267122526584No Hit
ATCTAAAGCTAAATATTGGC274590.113217316679089No Hit
AAAAAGGTGTTAGTTC274350.11311836130561226No Hit
CGATCAACTTAGAACTGGT272660.11242155055071344No Hit
TTTGGAATGAGTACAATGT271550.11196388194838348No Hit
ATAGGAATGTAGCTTGCCT271230.11183194145041447No Hit
ACGGGGTATTGTAAGC268780.1108217720128393No Hit
TATAGATGGATACGAATAAGGCGTCC266450.1098610802620025No Hit
AGGTTGGGGCCTCGCAAGAGGTGC264050.10887152652723497No Hit
AGGTCGTACTGATAACCGC262440.10820770089682843No Hit
ATATAGATGGATACGAATAAGGCGTCC261870.10797268188482115No Hit
AAATCGACCGATCCTGA259310.10691715790106912No Hit
ATGAAAGTGTGGCCT256640.10581627937114024No Hit
TCCACGTTCTAGCATTCAAGGTCCCA255450.10532562564431804No Hit
TGTTTTCATGCCAGGTCGT254470.10492155786928795No Hit
ATGTAAGAAGTCCTTGTTACTT252800.10423299339551223No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGC251540.10371347768475926No Hit
ACGTAATGTGAATTGCAGA250740.10338362643983676No Hit
TATATAGATGGATACGAAT243290.10031188672149592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)