FastQCFastQC Report
Sun 2 Jul 2023
SRR3296796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27395024
Total Bases638.3 Mbp
Sequences flagged as poor quality0
Sequence length14-36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCCA5673852.0711243034501448No Hit
TCACGTTCTAGCATTCAAGGTCCC4788531.7479561251707612No Hit
AAGAGGTGCACAATCGACCGATCCTGA3330521.215739033482869No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT2694950.9837370465526879No Hit
CACGTTCTAGCATTCAAGGTCCCA2368020.8643978556105664No Hit
AAAAGCGAATGATTAGAGGT2333610.8518371803580095No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC2176240.7943924414886441No Hit
AAATATAGATGGATACGAATAAGGCGTC2166660.7908954560507047No Hit
AGTTATCGTTTATTTGATAGTT2049910.7482782274620384No Hit
CACGTTCTAGCATTCAAGGTCCC2033350.7422333340536589No Hit
AAGAGGTGCACAATCGACCGATCCTG1787510.6524944092036568No Hit
CCACGTTCTAGCATTCAAGGTCCCA1524040.5563200090644199No Hit
AGTCAAGGATGCTGGC1520350.5549730491201614No Hit
AAATTCTAGAGCTAATACATGCT1456150.5315381362688348No Hit
AGACACCACAAAAGGTGTTAGTTC1418530.5178057153737117No Hit
ATATAGATGGATACGAATAAGGCGTC1398390.5104540152985447No Hit
CTACATGGTATAACTGTGGT1389530.5072198513131436No Hit
AAATATAGATGGATACGAAT1372870.5011384549252448No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC1310360.4783204424277927No Hit
TATAGATGGATACGAATAAGGCGTC1308630.47768894088211056No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1275410.4655626510858322No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1244210.45417372147584173No Hit
CCACGTTCTAGCATTCAAGGTCCC1203310.4392440028524888No Hit
TCACGTTCTAGCATTCAAGGTCC1191690.4350023566323577No Hit
AAAATCAGTTATCGTTTATTTGATAGTT1180530.430928624118015No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1065730.3890232036299731No Hit
ATTAGCATGGAATAATAGAAT1059890.38689142962605183No Hit
ACGTTCTAGCATTCAAGGTCCCA1050690.3835331555102854No Hit
GTTTTCATGCCAGGTCGT1029550.37581642563992645No Hit
ATGTCTAAGTATAAGCAAT974460.355706934222799No Hit
ATTGTAATTATTGCTCTTC958640.3499321628628615No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT925390.33779492217272744No Hit
TATATAGATGGATACGAATAAGGCGTC910370.3323121746489435No Hit
ACGTTCTAGCATTCAAGGTCCC904350.3301146952818877No Hit
TTTTACTTTGAAAAAATTAGAGTGTTC868070.3168714143123218No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA831430.30349672261648686No Hit
TTGGAATGAGTACAATGTAAATACCTT813640.29700284256002113No Hit
TTATAGATGGATACGAATAAGGCGTC787810.2875741229502117No Hit
ATCCTGTCTGTGACGCCA787430.2874354116280387No Hit
AGATGGATACGAATAAGGCGTC761340.27791178427147933No Hit
AAATATAGATGGATACGAATAAGGCGTCC729850.2664169960208832No Hit
TCTACATGGTATAACTGTGGT688950.2514872773975303No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC677350.24725293177330307No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC661820.24158401905397128No Hit
AAAAGCGAATGATTAGAGGTT644700.23533470896028416No Hit
AGAAGCTCGTATCAGTTTT643120.23475796188388082No Hit
TAGATGGATACGAATAAGGCGTC638300.23299851827105533No Hit
CAAGAGGTGCACAATCGACCGATCCTG628690.22949058193926022No Hit
ATTGCTCGAATATATTAGC606710.22146722704093996No Hit
TTGGGCCAGCATCAGTTTTGGTGGC597310.21803594696613518No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC585300.2136519391258792No Hit
AAAGAGGTGCACAATCGACCGATCCTGA584630.21340736916310057No Hit
CCTGCGGAAGGATCATTA581270.21218086905125544No Hit
TTTAACGATCAACTTAGAACTGGT580650.2119545505782364No Hit
TCCACGTTCTAGCATTCAAGGTCCCA563430.2056687375050301No Hit
AGAAAGTGATGTTGACGC545090.19897409106120878No Hit
ATGCATGTCTAAGTATAAGCAA544550.1987769749718051No Hit
TAAGAGGTGCACAATCGACCGATCCTGA541350.1976088796271907No Hit
AAGGACGTTTTCATTAATC539190.19682041526957594No Hit
CACGTTCTAGCATTCAAGGTCC535820.1955902648597789No Hit
AAGCGGAGGAAAAGAAACC518060.18910733569716895No Hit
TCAATATAGATGGATACGAATAAGGCGTC516790.1886437478572751No Hit
ATGGCTGGAAGAGGCCAGCA516690.1886072448777559No Hit
ATAGATGGATACGAATAAGGCGTC505710.18459921772654772No Hit
ATTAATCAAGAACGAAAGTT501510.18306609258674128No Hit
ACAATCGACCGATCCTGA494030.18033566971870513No Hit
ATATAGATGGATACGAATAAGGCGTCC486460.17757239416910164No Hit
ACTAAATAGTGGTGCTAGC475990.1737505322134414No Hit
AAGAGGTGCACAATCGACCGATC474510.17321028811655723No Hit
TCCCGCTAGAGACCACCA467180.17053461971779985No Hit
AAGGAGTGTGTAACAACTC464670.16961839493186792No Hit
ACGATCAACTTAGAACTGGT463530.16920226096534902No Hit
TTGGAATGAGTACAATGTAAAT461560.1684831522688208No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGC458640.16741726526686015No Hit
TATAGATGGATACGAATAAGGCGTCC450080.1642926102200166No Hit
TTTGCTCGAATATATTAGC442670.16158773943764385No Hit
AGAGGTGCACAATCGACCGATCCTGA442290.1614490281154709No Hit
TCCACGTTCTAGCATTCAAGGTCCC434730.15868940286381936No Hit
ATATTAGCATGGAATAATAGAAT415800.15177938884083475No Hit
ATGGAGAACAGAAATCTCCAGT412370.15052733664332615No Hit
AGAATAGGACGTTTGGTTCTA410810.14995789016282665No Hit
AGTTTTCATGCCAGGTCGT408150.1489869109076159No Hit
ACTTTGAAAAAATTAGAGTGTTC406330.14832255668036648No Hit
ATAAATCATTTGTATACGACTT403280.14720921580503088No Hit
TTGCTCGAATATATTAGC401590.14659231545115636No Hit
AAATGAAAGTGAACGT401190.14644630353307958No Hit
ATGCATGTCTAAGTATAAGCAAT400990.1463732975740412No Hit
ACCTGCGGAAGGATCATTA395790.14447514263904276No Hit
TTTGACCTCAAATCAGGT395770.14446784204313895No Hit
TGACTTTTACTTTGAAAAAATTAGAGTGTTC384890.14049631787144992No Hit
TCGCTTGCTACAATTAACGATC384150.14022619582300785No Hit
ATGGATTTGAGTAAGAGC381840.13938297699611432No Hit
AGAATGGGCGCTTGTCGCGTGCC378020.13798856317848088No Hit
ATGATGCCCTGACGAGT377080.13764543517100042No Hit
ATAGGAATGTAGCTTGCCT375410.13703583541302974No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGC373390.1362984752267419No Hit
TTTTCCACGTTCTAGCATTCAAGGTC369860.13500992004971415No Hit
TCAATATAGATGGATACGAAT355600.12980459517027618No Hit
AGAGGTGCACAATCGACCGATC355390.12972793891328588No Hit
TTGGAATGAGTACAATGT353600.12907453557989218No Hit
ACGTAATGTGAATTGCAGA350220.1278407348721432No Hit
TATGATGCCCTGACGAGT349400.12754141044008577No Hit
AGAGATGTATTTATTAGATA348580.12724208600802833No Hit
AAATAAGCGGAGGAAAAGAAACC346980.12665803833572112No Hit
ATCCGGGTAGGACCTCCA345030.1259462302350967No Hit
AGTTTTATGAGGTAAAGC344870.12588782546786598No Hit
ATAATAACTTTTCGAATCGC333840.12186154682689819No Hit
AAAGAGGTGCACAATCGACCGATCCTG332930.12152936971327347No Hit
TGTGCCCGAGTTGTAATTTG332700.12144541286037933No Hit
AAATTCTAGAGCTAATACATGCTT328290.11983563146358259No Hit
AGCATGGAATAATAGAAT326210.11907636948958321No Hit
TATATAGATGGATACGAATAAGGCGTCC324950.1186164319476413No Hit
TAGTTATCGTTTATTTGATAGTT321820.11747388868869031No Hit
AAATAGAATAGGACGTTTGGTTCTA313790.11454269943329855No Hit
TAAGAGGTGCACAATCGACCGATCCTG312120.11393309967532789No Hit
GTGCCCGAGTTGTAATTTG307290.11217000576455054No Hit
TTAGATGGATACGAATAAGGCGTC307190.11213350278503131No Hit
TATTAGCATGGAATAATAGAAT306580.11191083460996419No Hit
AAATCGACCGATCCTGA306540.11189623341815652No Hit
AAGCCTTTGTTGTCTGATTTGT306040.11171371852056053No Hit
CCACGTTCTAGCATTCAAGGTCC302930.1105784758575134No Hit
AGGCGTATTGCTCGAATATATTAGC301570.11008203533605226No Hit
TTTCCACGTTCTAGCATTCAAGGTCC301500.11005648325038883No Hit
ATTAGCATGGAATAATAGAATA299860.10945783438627395No Hit
AGATGGATACGAATAAGGCGTCC299500.10932642366000482No Hit
TAATATAGATGGATACGAATAAGGCGTC297660.10865476883685155No Hit
AAAAGCGAATGATTAGAGG296980.10840654857612099No Hit
ATCCGATTGGAAGCACCA296750.10832259172322682No Hit
TACATGGTATAACTGTGGT296400.10819483129490962No Hit
AAGCGTGTTACCTATACTCT293010.10695738028920873No Hit
GCACGTTCTAGCATTCAAGGTCCCA288840.10543520604325808No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGC285140.10408459580104766No Hit
TAAGGACGTTTTCATTAATC284170.10373051689971141No Hit
TTTTGATGATTCATAATAACTTTTCGAATC283210.1033800882963271No Hit
ACAATCGACCGATCCTG275770.1006642666200986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)