FastQCFastQC Report
Sun 2 Jul 2023
SRR3296797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45219392
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length14-36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA11439212.5297133583750973No Hit
TCACGTTCTAGCATTCAAGGTCCC9008501.992176276938885No Hit
TCACGTTCTAGCATTCAAGGTCCCA7858381.7378340690648826No Hit
AAGAGGTGCACAATCGACCGATCCTG5733881.2680135106637436No Hit
AAATATAGATGGATACGAATAAGGCGTC5231571.1569306371921144No Hit
AGTTATCGTTTATTTGATAGTT4118860.9108614286543261No Hit
CACGTTCTAGCATTCAAGGTCCC3908740.8643946384772268No Hit
TATAGATGGATACGAATAAGGCGTC3797650.8398277446985576No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3717820.8221738142786175No Hit
ATATAGATGGATACGAATAAGGCGTC3695730.8172887419627403No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT3686160.8151723932953368No Hit
TCACGTTCTAGCATTCAAGGTCC3471590.7677215120450979No Hit
CACGTTCTAGCATTCAAGGTCCCA3266730.7224179396308557No Hit
CTACATGGTATAACTGTGGT2962070.6550441898909212No Hit
CCACGTTCTAGCATTCAAGGTCCC2390840.5287200677090041No Hit
TATATAGATGGATACGAATAAGGCGTC2383480.5270924474172497No Hit
TTATAGATGGATACGAATAAGGCGTC2325130.5141886914357451No Hit
CCACGTTCTAGCATTCAAGGTCCCA2274380.502965630320726No Hit
ACGTTCTAGCATTCAAGGTCCC2195480.48551736387786903No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC2122280.46932961858487615No Hit
AGATGGATACGAATAAGGCGTC2093030.46286115478952033No Hit
AAATATAGATGGATACGAATAAGGCGTCC2081770.46037107265838517No Hit
AGACACCACAAAAGGTGTTAGTTC2042780.45174866570519123No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2033490.449694237374974No Hit
CAAGAGGTGCACAATCGACCGATCCTG1970550.4357754301517367No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT1924070.425496654178809No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1886500.41718827179277423No Hit
AAAATCAGTTATCGTTTATTTGATAGTT1863220.4120400380438552No Hit
ACGTTCTAGCATTCAAGGTCCCA1836740.4061841432985211No Hit
TAGATGGATACGAATAAGGCGTC1835270.405859061528293No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1822530.4030416861863158No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA1793340.3965864910346428No Hit
AAAAGCGAATGATTAGAGGT1638150.36226714414912964No Hit
CACGTTCTAGCATTCAAGGTCC1566040.3463204458830406No Hit
TCTACATGGTATAACTGTGGT1551790.34316914300838014No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1547840.3422956239659304No Hit
AGAGGTGCACAATCGACCGATCCTGA1520610.3362738711745616No Hit
ATATAGATGGATACGAATAAGGCGTCC1491170.3297633900075437No Hit
TATAGATGGATACGAATAAGGCGTCC1475120.3262140278223997No Hit
AGTCAAGGATGCTGGC1415730.3130802820170603No Hit
AAATTCTAGAGCTAATACATGCT1389910.3073703423522368No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC1343730.29715790959772304No Hit
TCAATATAGATGGATACGAATAAGGCGTC1331730.2945041808611668No Hit
TGTGCCCGAGTTGTAATTTG1259140.27845133344561557No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA1226660.2712685743320034No Hit
GTGCCCGAGTTGTAATTTG1223930.2706648510444368No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC1179150.26076201997585463No Hit
TTTTCCACGTTCTAGCATTCAAGGTC1101710.24363662386261187No Hit
AAAGAGGTGCACAATCGACCGATCCTG1078670.23854146468842397No Hit
ATAGATGGATACGAATAAGGCGTC1048370.23184079962861953No Hit
TTGGAATGAGTACAATGTAAATACCTT1033690.22859440480756574No Hit
TATATAGATGGATACGAATAAGGCGTCC1013420.22411181468339952No Hit
CCTGCGGAAGGATCATTA1008970.22312772361025995No Hit
TAAGAGGTGCACAATCGACCGATCCTG1005890.22244659990121055No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC997730.2206420643603523No Hit
ACGTTCTAGCATTCAAGGTCC938980.20764985075429587No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA938480.20753927872360603No Hit
AAGAGGTGCACAATCGACCGATC927950.20521063175727794No Hit
TCCACGTTCTAGCATTCAAGGTCCCA919100.20325350681406773No Hit
TTGGGCCAGCATCAGTTTTGGTGGC915070.20236229624670762No Hit
ATTAGCATGGAATAATAGAAT909600.20115263823096072No Hit
AGATGGATACGAATAAGGCGTCC896990.1983640116169629No Hit
TTAGATGGATACGAATAAGGCGTC896380.1982291137395213No Hit
TCCACGTTCTAGCATTCAAGGTCCC864280.19113038936923343No Hit
CCACGTTCTAGCATTCAAGGTCC862370.19070800421199824No Hit
CCTGATTCAAGCTCAGGTA856380.18938335128433392No Hit
TTATAGATGGATACGAATAAGGCGTCC852960.18862703859441543No Hit
TTTCCACGTTCTAGCATTCAAGGTCC820870.18153052566474134No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA814580.18013952951866313No Hit
AGAGATGTATTTATTAGATA803180.17761848721893475No Hit
AGAGGTGCACAATCGACCGATCCTG796900.17622970251347034No Hit
TTTGCTCGAATATATTAGC795640.17595106099613192No Hit
GTTTTCATGCCAGGTCGT764110.1689783887408305No Hit
ATCCTGTCTGTGACGCCA757530.16752326081695218No Hit
ATGCATGTCTAAGTATAAGCAA753270.1665811871154747No Hit
TTGCTCGAATATATTAGC744320.16460194776612652No Hit
AGAGGTGCACAATCGACCGATC736000.16276202917544755No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG733540.16221801478445355No Hit
TTTTACTTTGAAAAAATTAGAGTGTTC726950.16076067541996142No Hit
TACATGGTATAACTGTGGT710880.1572068903535899No Hit
TAATATAGATGGATACGAATAAGGCGTC710210.1570587238324655No Hit
ATGCATGTCTAAGTATAAGCAAT708570.1566960475718028No Hit
CCTGATTCAAGCTCAGGT701760.15519005651380716No Hit
TAGATGGATACGAATAAGGCGTCC697530.1542546171341711No Hit
TGACTTTTACTTTGAAAAAATTAGAGTGTTC684340.15133772696457307No Hit
TAGTTATCGTTTATTTGATAGTT663610.1467534105721722No Hit
TCTTCTTCATGATAAGACAGTT657090.14531155129197668No Hit
AAATATAGATGGATACGAAT652450.14428544284717496No Hit
ACCTGCGGAAGGATCATTA633960.14019648915226457No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGC632050.13977410399502938No Hit
TTGGAATGAGTACAATGTAAAT629220.13914826630132487No Hit
CAATATAGATGGATACGAATAAGGCGTC627730.13881876164986914No Hit
TCAGATTTAGGCTCTGATA627630.1387966472437312No Hit
AAATCGACCGATCCTGA612080.1353578570892771No Hit
TGCCCGAGTTGTAATTTG610080.13491556896651774No Hit
ATGGAGAACAGAAATCTCCAGT606720.13417252492028198No Hit
ACAATCGACCGATCCTGA597600.13215569108049927No Hit
AATATAGATGGATACGAATAAGGCGTC597590.13215347963988547No Hit
ATAAATCATTTGTATACGACTT590380.13055903095733795No Hit
AAATGAAAGTGAACGT565820.1251277328098529No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGC556840.12314185913866334No Hit
TTTGTGGCGTCGCTGAACCATAGC545150.12055668506113484No Hit
TTTTGATGATTCATAATAACTTTTCGAATC544200.12034659820282415No Hit
TCAATATAGATGGATACGAATAAGGCGTCC541510.1197517206777128No Hit
AGAATAGGACGTTTGGTTCTA535620.11844918215618645No Hit
TTTGACCTCAAATCAGGT531320.1174982626922538No Hit
TTTAACGATCAACTTAGAACTGGT522960.11564949833911965No Hit
AAAAGCGAATGATTAGAGGTT516780.11428282803979319No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG510860.11297365519642545No Hit
ATGGCTGGAAGAGGCCAGCA509410.11265299630742492No Hit
TTTCCACGTTCTAGCATTCAAGGTC505600.11181043743356833No Hit
ATTAATCAAGAACGAAAGTT497220.10995725019920657No Hit
TTGGAATGAGTACAATGT492740.10896652480422558No Hit
AAGAGGTGCACAATCGACCGATCCT473770.10477142195985298No Hit
TTTGCTCGAATATATT453150.10021143141420388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)