FastQCFastQC Report
Sun 2 Jul 2023
SRR3296798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28004052
Total Bases639.7 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCC7723672.7580544415501014No Hit
AAGAGGTGCACAATCGACCGATCCTGA6735112.4050483837124714No Hit
TCACGTTCTAGCATTCAAGGTCCCA5216701.8628375636497176No Hit
CACGTTCTAGCATTCAAGGTCCC5060861.8071884740108326No Hit
AAGAGGTGCACAATCGACCGATCCTG4274521.5263933947844404No Hit
CACGTTCTAGCATTCAAGGTCCCA3312001.1826859912986878No Hit
AAATATAGATGGATACGAATAAGGCGTC3259081.1637887260029371No Hit
ATATAGATGGATACGAATAAGGCGTC2687480.959675406973248No Hit
TATAGATGGATACGAATAAGGCGTC2610680.9322508042764669No Hit
TTTGACCTCAAATC2489040.8888142330259922No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT2470760.8822866062382686No Hit
TCACGTTCTAGCATTCAAGGTCC2260890.8073438800927809No Hit
GTGCCCGAGTTGTAATTTG2140670.7644143783192517No Hit
CCACGTTCTAGCATTCAAGGTCCC1980320.7071548074542927No Hit
ACGTTCTAGCATTCAAGGTCCC1950770.696602763057289No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1852740.6615971145889887No Hit
TGTGCCCGAGTTGTAATTTG1837350.6561014813142041No Hit
AGTCAAGGATGCTGGC1835180.6553265934515476No Hit
TTGCTCGAATATATTAGC1781370.6361115170047534No Hit
CACGTTCTAGCATTCAAGGTCC1573310.561815125896781No Hit
TATATAGATGGATACGAATAAGGCGTC1533710.5476743151312531No Hit
ACGTTCTAGCATTCAAGGTCCCA1414000.5049269298600074No Hit
CCACGTTCTAGCATTCAAGGTCCCA1411890.5041734674682078No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT1362400.48650102492310754No Hit
AGTTATCGTTTATTTGATAGTT1336360.4772023705712302No Hit
TTATAGATGGATACGAATAAGGCGTC1319150.47105683134712073No Hit
AGATGGATACGAATAAGGCGTC1312580.46871074228829457No Hit
AAATATAGATGGATACGAATAAGGCGTCC1304550.4658433001052848No Hit
TTTGCTCGAATATATTAGC1296790.46307227254113087No Hit
TGCCCGAGTTGTAATTTG1277310.45611613633627024No Hit
CAAGAGGTGCACAATCGACCGATCCTG1271780.4541414221056295No Hit
TAGATGGATACGAATAAGGCGTC1262420.45079904865195936No Hit
CTACATGGTATAACTGTGGT1163690.41554343635699575No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC1157970.4135008748019751No Hit
ATATAGATGGATACGAATAAGGCGTCC1098170.39214682218130437No Hit
ACAATCGACCGATCCTGA1033510.3690573064212279No Hit
ATAGATGGATACGAATAAGGCGTC1013710.36198690103846404No Hit
AAAATCAGTTATCGTTTATTTGATAGTT995900.355627107105786No Hit
AAAGAGGTGCACAATCGACCGATCCTGA987560.35264896665668244No Hit
TATAGATGGATACGAATAAGGCGTCC985200.35180623146964585No Hit
AAAAGCGAATGATTAGAGGT961080.34319319218518807No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC957060.3417576856377784No Hit
TTTTGATGATTCAT954040.34067927027131645No Hit
AGAGGTGCACAATCGACCGATCCTGA941680.3362656232748032No Hit
TTGCCCGAGTTGTAATTTG923770.3298701202240304No Hit
AAATTCTAGAGCTAATACATGCT847110.3024955102925819No Hit
TAAGAGGTGCACAATCGACCGATCCTGA809560.2890867364479969No Hit
TTTAACGATCAACTTAGAACTGGT767070.27391393216953036No Hit
ATTAGCATGGAATAATAGAAT749250.26755056732504284No Hit
ATGGAATAATAGAAT695320.2482926399365349No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC687250.24541091410628718No Hit
TCAATATAGATGGATACGAATAAGGCGTC686360.24509310295524378No Hit
AAAGAGGTGCACAATCGACCGATCCTG662310.2365050600534523No Hit
TATATAGATGGATACGAATAAGGCGTCC642240.2293382400518325No Hit
ACGTTCTAGCATTCAAGGTCC628270.22434967625399355No Hit
AAATATAGATGGATACGAAT625840.22348194468429067No Hit
GTTTTCATGCCAGGTCGT610640.2180541587338861No Hit
AGAGGTGCACAATCGACCGATCCTG579100.20679150288679654No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC569920.20351340584569688No Hit
ACGATCAACTTAGAACTGGT568060.2028492162491342No Hit
CCACGTTCTAGCATTCAAGGTCC554790.19811061627795865No Hit
TAAGAGGTGCACAATCGACCGATCCTG547790.19561097801132493No Hit
TTTGCTCGAATATATT543090.19393264946087088No Hit
AGCATGGAATAATAGAAT541380.19332202354145037No Hit
AAATCGACCGATCCTGA535340.19116519280852642No Hit
AGATGGATACGAATAAGGCGTCC530030.1892690386376943No Hit
ATGGAGAACAGAAATCTCCAGT517030.18462685328537456No Hit
AGACACCACAAAAGGTGTTAGTTC511170.1825342989650212No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA508890.18172013107246052No Hit
TTTTACTTTGAAAAAATTAGAGTGTTC505210.18040603552657308No Hit
TCCACGTTCTAGCATTCAAGGTCCC504610.1801917808180045No Hit
TTATAGATGGATACGAATAAGGCGTCC492900.17601024308910726No Hit
TTGGAATGAGTACAATGTAAATACCTT490610.17519250428473707No Hit
TTAGATGGATACGAATAAGGCGTC488660.17449617648188911No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC488660.17449617648188911No Hit
AATATAGATGGATACGAATAAGGCGTC486790.17382841597351698No Hit
AGTTGAACTTTGGG482220.1721965092765861No Hit
CAATATAGATGGATACGAATAAGGCGTC474560.16946119083052694No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA473070.16892912497091492No Hit
TAGATGGATACGAATAAGGCGTCC459400.16404768852736026No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA458940.1638834265841243No Hit
ATGTCTAAGTATAAGCAAT447540.15981258712132088No Hit
TATGATGCCCTGACGAGT444150.15860204801790828No Hit
ACGAATAAGGCGTC440550.15731651976649666No Hit
ATGGAATAATAGAATA430830.15384559348768528No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC430010.1535527787193082No Hit
ATTGCTCGAATATATTAGC429640.15342065498235755No Hit
ACTGATAACCGCAGC425860.15207085031837536No Hit
TCCACGTTCTAGCATTCAAGGTCCCA416630.14877489871822835No Hit
ACAATCGACCGATCCTG412680.14736438855348505No Hit
ATCCTGTCTGTGACGCCA411400.14690731184187203No Hit
ATAGATGGATACGAATAAGGCGTCC410710.14666091892701813No Hit
TTTTCCACGTTCTAGCATTCAAGGTC408610.14591102744702802No Hit
AAATGAAAGTGAACGT400100.14287218149716333No Hit
ATGATGCCCTGACGAGT399360.14260793402326205No Hit
GCACGTTCTAGCATTCAAGGTCCC389970.13925484783416342No Hit
TCTACATGGTATAACTGTGGT380910.13601960173477753No Hit
TAATATAGATGGATACGAATAAGGCGTC377240.13470907710069957No Hit
ATTAATCAAGAACGAAAGTT370030.13213444968606686No Hit
TTTGACCTCAAATCAGGT367710.13130599814626828No Hit
ATGGATTTGAGTAAGAGC359430.12834928316802155No Hit
ATGCATGTCTAAGTATAAGCAA346610.12377137422827239No Hit
AAGCGGAGGAAAAGAAACC342140.1221751766494363No Hit
AAATCGACCGATCCTG341670.12200734379439089No Hit
AGGTGCTGCAATCT338960.12103962669402271No Hit
ATATAGATGGATACGAAT337150.12039329165650743No Hit
TTGGGCCAGCATCAGTTTTGGTGGC334210.11934344358452127No Hit
TTTCCACGTTCTAGCATTCAAGGTCC331470.11836501374872464No Hit
CCTGATTCAAGCTCAGGT328650.11735801661845222No Hit
AAGGAGTGTGTAACAACTC326820.11670453975731797No Hit
TATGACTCTTAATCATA313830.11206592531680772No Hit
TATTTGATCAGACA308700.11023404755854617No Hit
AACGATCAACTTAGAACTGGT306920.10959842525645931No Hit
AGTCAAGGATGCTGGCAT304270.10865213362694799No Hit
ATGTCTAAGTATAAGCAA304130.1086021408616153No Hit
TTTTGATGATTCATAATAACTTTTCGAATC303930.10853072262542578No Hit
ATGGCTGGAAGAGGCCAGCA303170.10825933332790556No Hit
TTGACCTCAAATCAGGT302910.10816648962085915No Hit
AAGAGGTGCACAATCGACCGATC293690.10487410893252162No Hit
ACCCGCTGAACTTAAGC293430.10478126522547523No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG286620.1023494742832216No Hit
TTAACGATCAACTTAGAACTGGT282320.1008139822051466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)