FastQCFastQC Report
Sun 2 Jul 2023
SRR3296799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31092119
Total Bases700.6 Mbp
Sequences flagged as poor quality0
Sequence length14-36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA8029032.5823360575713736No Hit
TCACGTTCTAGCATTCAAGGTCCC7635962.4559149538826865No Hit
AAGAGGTGCACAATCGACCGATCCTG5436771.748600666297463No Hit
CACGTTCTAGCATTCAAGGTCCC5425501.7449759535527316No Hit
TCACGTTCTAGCATTCAAGGTCCCA5250921.6888266766250315No Hit
CACGTTCTAGCATTCAAGGTCCCA3628951.1671607200525638No Hit
AAATATAGATGGATACGAATAAGGCGTC2968260.954666357735219No Hit
TTTGACCTCAAATC2597550.83543678705205No Hit
ATATAGATGGATACGAATAAGGCGTC2285680.7351316261204327No Hit
TATAGATGGATACGAATAAGGCGTC2252100.7243314616157233No Hit
ACGTTCTAGCATTCAAGGTCCC2183200.7021715052615102No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT2142930.6892196701035397No Hit
CAAGAGGTGCACAATCGACCGATCCTGA2060460.6626952637097523No Hit
TCACGTTCTAGCATTCAAGGTCC2036950.6551338620568125No Hit
AGTCAAGGATGCTGGC1937020.6229938847204335No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1875820.6033104401793908No Hit
TTGCTCGAATATATTAGC1816340.5841801904849264No Hit
CCACGTTCTAGCATTCAAGGTCCC1798510.5784456183253384No Hit
ACGTTCTAGCATTCAAGGTCCCA1657490.5330900734041317No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC1653930.531945088721679No Hit
CACGTTCTAGCATTCAAGGTCC1533440.49319250321922414No Hit
TTTGCTCGAATATATTAGC1532180.4927872558316144No Hit
AAAAGCGAATGATTAGAGGT1513780.4868693574728695No Hit
ATTAGCATGGAATAATAGAAT1391570.44756357712383643No Hit
CCACGTTCTAGCATTCAAGGTCCCA1391400.44750890088900025No Hit
AGTTATCGTTTATTTGATAGTT1385380.44557271892597605No Hit
CAAGAGGTGCACAATCGACCGATCCTG1350190.4342547383148765No Hit
TATATAGATGGATACGAATAAGGCGTC1330150.42780937510241746No Hit
TAGATGGATACGAATAAGGCGTC1326520.42664187667620856No Hit
AGATGGATACGAATAAGGCGTC1284040.41297925046536715No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC1235720.3974383347754459No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1184710.3810322480754689No Hit
CTACATGGTATAACTGTGGT1170700.37652628307514197No Hit
AGAGGTGCACAATCGACCGATCCTGA1164710.3745997498594419No Hit
TTATAGATGGATACGAATAAGGCGTC1163000.3740497712619716No Hit
AAATTCTAGAGCTAATACATGCT1106460.3558650988052631No Hit
GTTTTCATGCCAGGTCGT1057900.34024699313674955No Hit
GTGCCCGAGTTGTAATTTG1028730.33086519448867413No Hit
ATAGATGGATACGAATAAGGCGTC1004330.32301754666512117No Hit
TAAGAGGTGCACAATCGACCGATCCTGA956750.30771463340919286No Hit
AGACACCACAAAAGGTGTTAGTTC943470.3034434545937509No Hit
TTTTGATGATTCAT935650.30092834779128436No Hit
ACGAATAAGGCGTC929190.2988506508675076No Hit
AAAGAGGTGCACAATCGACCGATCCTG852030.27403407275007535No Hit
AGGTGCTGCAATCT842850.271081556068919No Hit
ATTGTAATTATTGCTCTTC839390.2699687338775463No Hit
AAAATCAGTTATCGTTTATTTGATAGTT825700.26556568884867576No Hit
AAATATAGATGGATACGAATAAGGCGTCC822930.26467478784575604No Hit
AAATATAGATGGATACGAAT813290.261574323705631No Hit
ACAATCGACCGATCCTGA800990.2576183373027744No Hit
AGAGGTGCACAATCGACCGATCCTG786720.2530287498256391No Hit
TGTGCCCGAGTTGTAATTTG741040.23833692390023337No Hit
AAGAGGTGCACAATCGACCGATC726830.23376663391774616No Hit
TAAGAGGTGCACAATCGACCGATCCTG689290.22169283476626342No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC689260.22168318601893938No Hit
TATTTGATCAGACA680390.21883037306013142No Hit
AGCATGGAATAATAGAAT678700.21828682696087712No Hit
TTTTACTTTGAAAAAATTAGAGTGTTC673400.21658221493362997No Hit
ATATAGATGGATACGAATAAGGCGTCC655540.21083799402671785No Hit
ACGTTCTAGCATTCAAGGTCC631480.20309969867283734No Hit
TGCCCGAGTTGTAATTTG625820.20127930167770167No Hit
TTGCCCGAGTTGTAATTTG619450.19923055099589707No Hit
ATGGAATAATAGAAT617980.1987577623770191No Hit
TATAGATGGATACGAATAAGGCGTCC616290.19821421627776478No Hit
AGAGGTGCACAATCGACCGATC609090.19589851691999507No Hit
ACTGATAACCGCAGC591790.19033440596313167No Hit
TATGATGCCCTGACGAGT564620.181595857136659No Hit
ATCCTGTCTGTGACGCCA564400.18152509965628266No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA563820.1813385572080179No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC563250.1811552310088611No Hit
TCAATATAGATGGATACGAATAAGGCGTC562320.18085611984181585No Hit
AAATGAAAGTGAACGT545310.17538528010908488No Hit
ATGTCTAAGTATAAGCAAT545200.17534990136889672No Hit
ATGATGCCCTGACGAGT543380.17476454403123826No Hit
AGTTGAACTTTGGG540000.1736774518327297No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC537120.17275117208962182No Hit
AAATCGACCGATCCTGA531610.17097901883110636No Hit
ATTGCTCGAATATATTAGC523570.1683931545482635No Hit
TTAGATGGATACGAATAAGGCGTC517350.1663926476030791No Hit
ATATTAGCATGGAATAATAGAAT512800.16492925425893296No Hit
ATGGAGAACAGAAATCTCCAGT501960.16144284022584632No Hit
ATGGCTGGAAGAGGCCAGCA498420.1603042880416095No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA498160.16022066556480116No Hit
TTGGAATGAGTACAATGTAAATACCTT495160.1592557908323971No Hit
CCACGTTCTAGCATTCAAGGTCC493130.15860289226347035No Hit
TTTGCTCGAATATATT492700.1584645935518258No Hit
ATGTCTAAGTATAAGCAA479080.15408406226671137No Hit
ATATAGATGGATACGAAT474670.1526656964100774No Hit
AAATCGACCGATCCTG469650.15105113935785464No Hit
AGAAAGTGATGTTGACGC459090.14765478029979237No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC456570.14684428552457296No Hit
ATGCATGTCTAAGTATAAGCAA447490.14392393133449669No Hit
AGATGGATACGAATAAGGCGTCC446950.14375025388266396No Hit
TCCACGTTCTAGCATTCAAGGTCCC444130.14284327163420416No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA435430.1400451349102324No Hit
GCACGTTCTAGCATTCAAGGTCCC434790.13983929496731953No Hit
AAGGACGTTTTCATTAATC418580.13462575516322964No Hit
AGAAGCTCGTATCAGTTTT415480.13362871793974543No Hit
AATATAGATGGATACGAATAAGGCGTC411990.13250624700104874No Hit
ATGAAAGTGTGGCCT410220.13193697090893033No Hit
ATAGGAATGTAGCTTGCCT399760.1285727743419482No Hit
TCCACGTTCTAGCATTCAAGGTCCCA399500.12848915186513984No Hit
ACAATCGACCGATCCTG399320.12843125938119562No Hit
ACTAAATAGTGGTGCTAGC394910.12701289352456163No Hit
ATTAGCATGGAATAATAGAATA392440.1262184799948823No Hit
TATATAGATGGATACGAATAAGGCGTCC391660.12596761256445727No Hit
CAATATAGATGGATACGAATAAGGCGTC384300.1236004532209593No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA381090.12256803725728697No Hit
AAGGAGTGTGTAACAACTC373880.12024912165040921No Hit
ATTAATCAAGAACGAAAGTT366870.11799453102569175No Hit
TTTTCCACGTTCTAGCATTCAAGGTC358440.11528323302763636No Hit
TAGATGGATACGAATAAGGCGTCC350380.11269093624657747No Hit
TCTACATGGTATAACTGTGGT349370.1123660950866681No Hit
AAGAGGTGCACAATCGACCGATCCT345240.11103778420505851No Hit
TAATATAGATGGATACGAATAAGGCGTC343250.11039775063256385No Hit
ACGGGGAAACTCACC342370.11011472071105866No Hit
ATGGATTTGAGTAAGAGC341480.10982847454044545No Hit
AAGCGGAGGAAAAGAAACC331960.10676660538961659No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTCAAAGC328610.10568916193843204No Hit
ATCAGTTTTGGTGGC318010.10227993788393772No Hit
TATTAGCATGGAATAATAGAAT315710.10154020058909463No Hit
AGTCAAGGATGCTGGCAT314610.10118641318721314No Hit
TTTGACCTCAAATCAGGT312850.10062035334420276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)