FastQCFastQC Report
Sun 2 Jul 2023
SRR3296800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73830111
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length14-36
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA33841634.583716527258099No Hit
CACGTTCTAGCATTCAAGGTCCC19816492.6840661258114595No Hit
CACGTTCTAGCATTCAAGGTCCCA19421352.630545957055381No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA15144972.0513269985467044No Hit
CAAGAGGTGCACAATCGACCGATCCTGA14242301.929063874765135No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC13596191.8415508003231904No Hit
AAGAGGTGCACAATCGACCGATCCTG10822691.4658910644195022No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC9753431.3210639761871683No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT8971841.2152006652136822No Hit
AGAGATGTATTTATTAGATA7466641.01132720767547No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA7170980.9712812161422865No Hit
CCACGTTCTAGCATTCAAGGTCCCA6547470.8868292233774375No Hit
CACGTTCTAGCATTCAAGGTCC6427810.8706217440198621No Hit
CCACGTTCTAGCATTCAAGGTCCC6339370.8586428916516189No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC6209850.8410999138278419No Hit
GTGCCCGAGTTGTAATTTG5127640.6945187987053142No Hit
CAAGAGGTGCACAATCGACCGATCCTG4998470.6770232270137044No Hit
TCACGTTCTAGCATTCAAGGTCCCA4894280.6629110986979283No Hit
AGAGATGTATTTATTAGAT4800890.6502617881747462No Hit
TCACGTTCTAGCATTCAAGGTCCC4543870.6154494336328439No Hit
ACGTTCTAGCATTCAAGGTCCCA4333560.5869637660439113No Hit
TCCACGTTCTAGCATTCAAGGTCCCA4288640.5808795275954549No Hit
TCCACGTTCTAGCATTCAAGGTCCC3916300.5304475297348531No Hit
TTTCCACGTTCTAGCATTCAAGGTCC3741230.5067349824247183No Hit
ACGTTCTAGCATTCAAGGTCCC3736880.5061457919249234No Hit
TTTTCCACGTTCTAGCATTCAAGGTC3279140.44414669781547533No Hit
TTCCACGTTCTAGCATTCAAGGTCCCA3198390.433209425893996No Hit
TTTTCCACGTTCTAGCATTC3074640.41644797202052153No Hit
ATATAGATGGATACGAATAAGGCGTC2885460.3908242803535809No Hit
TTCCACGTTCTAGCATTCAAGGTCCC2723980.3689524454324605No Hit
TTTTCCACGTTCTAGC2711670.367285104041087No Hit
AGAGGTGCACAATCGACCGATCCTGA2681560.36320682221377126No Hit
TGTTGTAGACGGCCTTGG2414040.32697228370684694No Hit
TTTCCACGTTCTAGC2136910.28943610825669763No Hit
TTTCCACGTTCTAGCATTC2054690.2782997305801152No Hit
AAGAGGTGCACAATCGACCGATCCT2049320.27757238506657533No Hit
AAGAGGTGCACAATCGACCGATC2035940.2757601163568615No Hit
TATAGATGGATACGAATAAGGCGTC1854360.25116581498841306No Hit
CCACGTTCTAGCATTCAAGGTCC1836070.24868850596743652No Hit
TGCCCGAGTTGTAATTTG1814450.2457601614604101No Hit
AGACACCACAAAAGGTGTTAGTTC1789310.2423550467098715No Hit
ATATAGATGGATACGAATAAGGCGTCC1778610.24090577352646808No Hit
ATGTATTTATTAGATAAAAAATCAATG1696950.22984524566135356No Hit
ATGTCTAAGTATAAGCAAT1607190.2176876044517934No Hit
AAATATAGATGGATACGAATAAGGCGTC1588900.21521029543081685No Hit
AATATAGATGGATACGAATAAGGCGTC1568260.21241468809385916No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC1556770.2108584124978493No Hit
ATAGATGGATACGAATAAGGCGTC1515340.2052468809101479No Hit
TTCCACGTTCTAGCATTCAAGGTCC1448230.19615709368227824No Hit
ACGTTCTAGCATTCAAGGTCC1431060.19383148428423735No Hit
TCCACGTTCTAGCATTCAAGGTCC1414040.19152619179998254No Hit
GTCTAGTGGTATGATTCTCGC1392380.18859242944927984No Hit
TCACGTTCTAGCATTCAAGGTCC1384500.18752511424505375No Hit
TCTTCTTCATGATAAGACAGTT1316700.17834186921376835No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA1286120.17419992772325643No Hit
GTGCCCGAGTTGTAATTTGG1196400.16204770435737256No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1194860.161839117375836No Hit
TTTCCACGTTCTAGCATTCAAGGTC1176240.15931711114453018No Hit
GTAGACGGCCTTGG1163140.15754276734055025No Hit
AGAGGTGCACAATCGACCGATC1145210.15511421891266017No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1095310.14835545892650764No Hit
TATAGATGGATACGAATAAGGCGTCC1080560.14635762907088137No Hit
ACAATCGACCGATCCTGA1075400.14565872723664197No Hit
AGTTATCGTTTATTTGATAGTT1071980.14519550160232048No Hit
CAATATAGATGGATACGAATAAGGCGTC1050970.14234977921135727No Hit
CTACATGGTATAACTGTGGT1033930.1400417778052643No Hit
CCTGATTCAAGCTCAGGTA997380.13509122314606842No Hit
TAAGAGGTGCACAATCGACCGATCCTGA989830.1340686051521716No Hit
AATCGACCGATCCTGA986570.13362705089255522No Hit
AGAGGTGCACAATCGACCGATCCTG971830.131630575497848No Hit
AATATAGATGGATACGAATAAGGCGTCC955480.12941603189517079No Hit
TAGATGGATACGAATAAGGCGTC945360.12804531744507333No Hit
ATGTATTTATTAGATAAAAAATCA944140.12788007321294695No Hit
ATCCTGTCTGTGACGCCA940920.12744393679700686No Hit
AAATATAGATGGATACGAATAAGGCGTCC902460.12223468010226886No Hit
TCGAAGAGTCGAGTTGTTT846360.11463615434629375No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG833810.1129363058928626No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAA818110.11080980224992484No Hit
ATAGATGGATACGAATAAGGCGTCC796550.10788958450841284No Hit
TTCCACGTTCTAGC760770.10304332333998523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)