FastQCFastQC Report
Sun 2 Jul 2023
SRR3296801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20482720
Total Bases470.6 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA5005552.443791644859667No Hit
TCACGTTCTAGCATTCAAGGTCCC4984312.433421928337643No Hit
TCACGTTCTAGCATTCAAGGTCCCA3685691.7994143355960537No Hit
CACGTTCTAGCATTCAAGGTCCC3102221.5145547075778998No Hit
AAGAGGTGCACAATCGACCGATCCTG2616651.277491465977175No Hit
AAATATAGATGGATACGAATAAGGCGTC2402911.1731400907691947No Hit
CACGTTCTAGCATTCAAGGTCCCA2220721.0841919432575362No Hit
ATATAGATGGATACGAATAAGGCGTC1827190.892064139918917No Hit
TATAGATGGATACGAATAAGGCGTC1767250.8628004483779497No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1680220.8203109743237227No Hit
GTGCCCGAGTTGTAATTTG1553740.758561362943984No Hit
TTTGACCTCAAATC1541670.7526685908902724No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1508430.7364402774631494No Hit
TGTGCCCGAGTTGTAATTTG1455310.7105062218299132No Hit
AGTCAAGGATGCTGGC1438970.702528765710804No Hit
TCACGTTCTAGCATTCAAGGTCC1431540.6989013177937305No Hit
CCACGTTCTAGCATTCAAGGTCCC1321930.6453879172297429No Hit
ACGTTCTAGCATTCAAGGTCCC1163870.56822043166142No Hit
TATATAGATGGATACGAATAAGGCGTC1066410.5206388604638447No Hit
AAATATAGATGGATACGAATAAGGCGTCC1057310.5161960911441449No Hit
CCACGTTCTAGCATTCAAGGTCCCA1041910.5086775584492684No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC987640.4821820539459603No Hit
TTATAGATGGATACGAATAAGGCGTC945820.4617648437316919No Hit
CACGTTCTAGCATTCAAGGTCC936210.4570730840435255No Hit
AGATGGATACGAATAAGGCGTC923020.4506335096120046No Hit
ACGTTCTAGCATTCAAGGTCCCA910550.4445454509947898No Hit
TTGCTCGAATATATTAGC894830.4368706890491107No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT861880.42078395838052757No Hit
CAAGAGGTGCACAATCGACCGATCCTG853490.4166878227110462No Hit
TAGATGGATACGAATAAGGCGTC843480.4118007764593765No Hit
AGTTATCGTTTATTTGATAGTT842980.4115566682549974No Hit
ATATAGATGGATACGAATAAGGCGTCC821340.4009916651694697No Hit
TTTTGATGATTCAT789910.3856470234421991No Hit
AAAGAGGTGCACAATCGACCGATCCTGA780830.38121401845067454No Hit
TGCCCGAGTTGTAATTTG752480.3673730832623792No Hit
TATAGATGGATACGAATAAGGCGTCC717290.3501927478381778No Hit
AGAGGTGCACAATCGACCGATCCTGA707420.3453740518837342No Hit
TTTGCTCGAATATATT693160.3384120858948421No Hit
TAAGAGGTGCACAATCGACCGATCCTGA674340.3292238530820126No Hit
TTTGCTCGAATATATTAGC667810.3260357999328214No Hit
AAAATCAGTTATCGTTTATTTGATAGTT659540.321998250232391No Hit
ATAGATGGATACGAATAAGGCGTC626160.3057015865080419No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC614690.3001017442995852No Hit
ACAATCGACCGATCCTGA608980.2973140286055758No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC583380.28481568854136563No Hit
ATGGAATAATAGAAT572630.27956736214721484No Hit
TCAATATAGATGGATACGAATAAGGCGTC565040.27586179960474No Hit
AGACACCACAAAAGGTGTTAGTTC564910.27579833147160143No Hit
AAATCGACCGATCCTGA535170.26127877547513223No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA504620.24636376418756883No Hit
CTACATGGTATAACTGTGGT500830.24451342399837522No Hit
TTTAACGATCAACTTAGAACTGGT495680.24199910949327044No Hit
TATATAGATGGATACGAATAAGGCGTCC494240.2412960778646586No Hit
AAAAGCGAATGATTAGAGGT478620.23367013755985533No Hit
ACGAATAAGGCGTC478590.2336554910675926No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC453300.22130849809009742No Hit
ATTAGCATGGAATAATAGAAT444160.21684620011404734No Hit
AAATATAGATGGATACGAAT432090.21095342806033576No Hit
AAAGAGGTGCACAATCGACCGATCCTG422770.2064032511307092No Hit
ATGGAGAACAGAAATCTCCAGT417450.20380593983611553No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA413950.2020971824054618No Hit
ATGGAATAATAGAATA411060.20068623698415053No Hit
AAATTCTAGAGCTAATACATGCT402650.196580336986494No Hit
AGTTGAACTTTGGG389840.1903262847903013No Hit
AGAGGTGCACAATCGACCGATCCTG385630.18827089370942923No Hit
TTATAGATGGATACGAATAAGGCGTCC384050.18749951178359126No Hit
ATTGCGCCCCTTGGTATTCCAGGGGGC383910.1874311614863651No Hit
TAAGAGGTGCACAATCGACCGATCCTG375000.18308115328432945No Hit
TTGCCCGAGTTGTAATTTG374570.1828712202285634No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC373260.18223165673309014No Hit
ACGTTCTAGCATTCAAGGTCC363300.17736902130185836No Hit
ACGATCAACTTAGAACTGGT362900.17717373473835504No Hit
CCACGTTCTAGCATTCAAGGTCC361910.17669040049368442No Hit
AGATGGATACGAATAAGGCGTCC360540.17602154401368567No Hit
CAATATAGATGGATACGAATAAGGCGTC353050.17236480311208666No Hit
TCCACGTTCTAGCATTCAAGGTCCC348720.17025082606216363No Hit
TTAGATGGATACGAATAAGGCGTC348220.1700067178577845No Hit
AATATAGATGGATACGAATAAGGCGTC342910.1674142887272784No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC332910.1625321246396963No Hit
AAATCGACCGATCCTG326020.1591683135833522No Hit
TTGGAATGAGTACAATGTAAATACCTT323580.15797706554598218No Hit
TCCACGTTCTAGCATTCAAGGTCCCA322490.15744490966043573No Hit
ACAATCGACCGATCCTG315260.15391510502511385No Hit
TTTTACTTTGAAAAAATTAGAGTGTTC314860.15371981846161056No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA312320.1524797487833647No Hit
TAATATAGATGGATACGAATAAGGCGTC304650.14873512892818924No Hit
TAGATGGATACGAATAAGGCGTCC301700.1472948905223525No Hit
TTGCTCGAATATATT299600.14626963606396023No Hit
ATGTCTAAGTATAAGCAAT291740.1424322550911207No Hit
AAATGAAAGTGAACGT291660.14239319777842008No Hit
TTTTCCACGTTCTAGCATTCAAGGTC290050.14160716936031933No Hit
GTTTTCATGCCAGGTCGT278930.13617820289492802No Hit
ACTGATAACCGCAGC277120.13529453119507567No Hit
AGAATGGGCGCTTGTCGCGTGCC276560.13502113000617105No Hit
GCACGTTCTAGCATTCAAGGTCCC275410.13445968113609913No Hit
ATAGATGGATACGAATAAGGCGTCC273990.13376641383566246No Hit
ATCCTGTCTGTGACGCCA269570.13160849730895113No Hit
AAGCGGAGGAAAAGAAACC267630.13066135747596022No Hit
ATATTAGCATGGAAT265230.12948963809494052No Hit
TTGGGCCAGCATCAGTTTTGGTGGC264280.1290258325066202No Hit
AGCATGGAATAATAGAAT263910.12884519243537967No Hit
TCAATATAGATGGATACGAATAAGGCGTCC249820.12196622323597647No Hit
TTTCCACGTTCTAGCATTCAAGGTCC246230.12021352632853449No Hit
AAGAGGTGCACAATCGACCGATC245970.12008659006225736No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA245860.12003288625729396No Hit
TTTTCCACGTTCTAGC243160.11871470195364679No Hit
TTTTGATGATTCATAATAACTTTTCGAATC241840.11807025629408593No Hit
ATTGCTCGAATATATTAGC238700.11653725677058516No Hit
ATGATGCCCTGACGAGT228740.11167462133935335No Hit
AAGAGGTGCACAATCGACCGATCCT228520.11156721372942656No Hit
AGCATTCAAGGTCCCA228160.1113914558222736No Hit
TTTGACCTCAAATCAGGT226380.11052243061468398No Hit
ACCCGCTGAACTTAAGC225540.11011232883132709No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG223060.10890155213760672No Hit
ATTAATCAAGAACGAAAGTT215560.10523992907192015No Hit
ACGGGGAAACTCACC211690.10335053157002586No Hit
ATGGCTGGAAGAGGCCAGCA211060.10304295523250817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)