FastQCFastQC Report
Sun 2 Jul 2023
SRR3296802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33234627
Total Bases731.5 Mbp
Sequences flagged as poor quality0
Sequence length14-36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA13333444.011912033795354No Hit
AAGAGGTGCACAATCGACCGATCCTG8488272.55404400958073No Hit
TCACGTTCTAGCATTCAAGGTCCC8335372.5080377763830475No Hit
CACGTTCTAGCATTCAAGGTCCC5582991.679871418445587No Hit
TCACGTTCTAGCATTCAAGGTCCCA4126981.2417711202234945No Hit
ACGAATAAGGCGTC3578001.0765879815651307No Hit
TTTGCTCGAATATATT3169720.9537402059604883No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3132130.9424297134431507No Hit
TCACGTTCTAGCATTCAAGGTCC2986090.8984875924739579No Hit
CACGTTCTAGCATTCAAGGTCCCA2675480.8050278403906865No Hit
TTGCTCGAATATATTAGC2669200.8031382449395325No Hit
AAATCGACCGATCCTGA2508150.7546797501292853No Hit
AAATATAGATGGATACGAATAAGGCGTC2342030.7046957379723263No Hit
TTTGCTCGAATATATTAGC2270160.6830707021324476No Hit
AAATCGACCGATCCTG2160250.6499997728272985No Hit
ACGTTCTAGCATTCAAGGTCCC2135010.6424052841032336No Hit
CACGTTCTAGCATTCAAGGTCC2109600.6347596439099497No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2081670.6263557584082409No Hit
CAAGAGGTGCACAATCGACCGATCCTG2046180.6156771369812576No Hit
AGAGGTGCACAATCGACCGATCCTGA2034820.6122590152734375No Hit
TATAGATGGATACGAATAAGGCGTC2016100.6066263358394244No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT2006310.6036806129943928No Hit
CCACGTTCTAGCATTCAAGGTCCC2004250.603060777543855No Hit
GTGCCCGAGTTGTAATTTG1859960.5596452158166241No Hit
TTTTGATGATTCAT1832910.5515061143908732No Hit
ATTAGCATGGAATAATAGAAT1818410.5471431949574761No Hit
TAAGAGGTGCACAATCGACCGATCCTGA1778760.5352128669896009No Hit
ATGGAATAATAGAAT1768680.5321798857559015No Hit
ATATAGATGGATACGAATAAGGCGTC1718050.5169457746584609No Hit
AGTCAAGGATGCTGGC1545280.4649608373820473No Hit
ATATTAGCATGGAAT1526580.4593341757679423No Hit
TGTGCCCGAGTTGTAATTTG1421310.4276593806814802No Hit
TTGCTCGAATATATT1411230.42462639944778074No Hit
TTTGACCTCAAATC1399990.42124438465940955No Hit
AAAGAGGTGCACAATCGACCGATCCTG1396850.4202995869338326No Hit
AGAGGTGCACAATCGACCGATCCTG1367880.41158277479690086No Hit
ACAATCGACCGATCCTGA1288550.38771309213128824No Hit
TTGCCCGAGTTGTAATTTG1263190.38008249648777465No Hit
AGATGGATACGAATAAGGCGTC1236170.3719524217918859No Hit
ACGTTCTAGCATTCAAGGTCCCA1227860.36945201762005636No Hit
TAAGAGGTGCACAATCGACCGATCCTG1200950.3613550409336624No Hit
TGCCCGAGTTGTAATTTG1165300.35062827694741394No Hit
AAGAGGTGCACAATCGACCGATC1128330.33950433684722864No Hit
TTTTCCACGTTCTAGC1114420.33531894310112165No Hit
TAGATGGATACGAATAAGGCGTC1099070.33070026632162897No Hit
CCACGTTCTAGCATTCAAGGTCCCA1093120.32890996489895913No Hit
ATGGAATAATAGAATA1068420.3214779573124139No Hit
AATCGACCGATCCTGA1054310.31723238536722553No Hit
TATATAGATGGATACGAATAAGGCGTC1011300.3042910636547839No Hit
TTATAGATGGATACGAATAAGGCGTC1003230.30186287332185197No Hit
ACAATCGACCGATCCTG963690.28996564336347147No Hit
AGATGGATACGAAT962200.28951731578031553No Hit
AGCATGGAATAATAGAAT953930.2870289472483022No Hit
ATAGATGGATACGAATAAGGCGTC876250.26365573472511067No Hit
AAGAGGTGCACAATCGACCGATCCT861360.2591754678035051No Hit
ACGTTCTAGCATTCAAGGTCC843590.2538286348151282No Hit
AATCGACCGATCCTG837220.2519119591743876No Hit
AAATCGACCGATCCT812190.2443806575593582No Hit
TTTCCACGTTCTAGC783590.2357751750907269No Hit
AAATATAGATGGATACGAATAAGGCGTCC774820.23313636106101024No Hit
ACGAATAAGGCGTCC766280.23056675196023715No Hit
AGCATTCAAGGTCCCA740580.22283385337828523No Hit
ATATTAGCATGGAATAATAGAAT725920.2184227913856232No Hit
AGAGGTGCACAATCGACCGATC725160.21819411422911414No Hit
CCACGTTCTAGCATTCAAGGTCC717680.21594344958347209No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC710970.2139244710042932No Hit
AGCATTCAAGGTCCC698530.210181387021434No Hit
TATAGATGGATACGAATAAGGCGTCC652040.19619296464497707No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT636970.19165853734419827No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC627840.1889114025561352No Hit
TTTGCTCGAATATATTAGCATGGAAT626930.1886375917503151No Hit
ATGCCTGTTTGAGC621880.18711809222351133No Hit
ATAGATGGATACGAAT614870.18500884634571044No Hit
AAATATAGATGGATACGAAT609320.18333890132120334No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC591830.17807631781154035No Hit
AGATGGATACGAATAAGGCGTCC589400.1773451526927021No Hit
ATATAGATGGATACGAATAAGGCGTCC580370.17462810700417972No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC571460.1719471682351061No Hit
ACAATCGACCGATC570940.17179070491749462No Hit
ATTAGCATGGAATAATAGAATA560530.168658429655311No Hit
ATGCCTGTTTGAGCGTC553400.16651307685806133No Hit
AGTTGAACTTTGGG543340.16348611344427003No Hit
AAATGAAAGTGAACGT536870.16153934870398878No Hit
ATTCAATGAAGCGGAG533330.16047419458024909No Hit
TCAATATAGATGGATACGAATAAGGCGTC517970.15585250889080235No Hit
TAGATGGATACGAAT516580.15543427040718708No Hit
GCACGTTCTAGCATTCAAGGTCCC508190.15290979495572493No Hit
TATTAGCATGGAATAATAGAAT505040.15196198832019386No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA497370.1496541543854246No Hit
TCCACGTTCTAGCATTCAAGGTCCC482250.14510468253487546No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC456120.1372424008248987No Hit
TTAGATGGATACGAATAAGGCGTC454420.1367308861327073No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA450220.1354671439519992No Hit
ATATAGATGGATACGAAT441560.1328614279317773No Hit
TTTTGATGATTCATAAT431600.1298645536175267No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA429040.12909427266928555No Hit
TTTCCACGTTCTAGCATTCAAGGTCC427840.12873320347479753No Hit
AGGTGCTGCAATCT427500.12863090053635926No Hit
AGTTATCGTTTATTTGATAGTT424850.12783353939853154No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA420810.12661793977708852No Hit
TTTTCCACGTTCTAGCATTCAAGGTC419810.12631704878168182No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA413380.12438231968121681No Hit
AAAAGCGAATGATTAGAGGT407080.12248670641015469No Hit
TATAGATGGATACGAAT403940.12154190868457768No Hit
ATTAGCATGGAATAAT372840.11218419872742967No Hit
TATGACTCTTAATCATA364080.10954839360766708No Hit
CTACATGGTATAACTGTGGT360950.10860660479204416No Hit
TAGATGGATACGAATAAGGCGTCC359410.10814323265911786No Hit
TATATAGATGGATACGAATAAGGCGTCC354020.10652143019387579No Hit
ACAATCGACCGATCCT353820.10646125199479446No Hit
ATGATGCCCTGACGAGT353800.10645523417488632No Hit
AATATAGATGGATACGAATAAGGCGTC348760.10493874355803662No Hit
AATCGACCGATCCT345800.10404810621163284No Hit
GTTTTCATGCCAGGTCGT341710.10281746204041947No Hit
TATTAGCATGGAAT339310.10209532365144341No Hit
ATATTAGCATGGAATAATAGAATA337780.10163496042847119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)