FastQCFastQC Report
Sun 2 Jul 2023
SRR3296803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47169195
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length14-36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA25003225.30075189962432No Hit
AAGAGGTGCACAATCGACCGATCCTG8248971.7488044898794648No Hit
AAATCGACCGATCCTGA7482281.5862640861265493No Hit
CAAGAGGTGCACAATCGACCGATCCTGA6652521.4103526676679559No Hit
ACGAATAAGGCGTC5929981.257172186211785No Hit
ATGGAATAATAGAAT4995951.0591552389223517No Hit
AGATGGATACGAAT4699490.9963048977197936No Hit
ATGTATTTATTAGATA4557120.9661220633508797No Hit
AAAGAGGTGCACAATCGACCGATCCTGA3914740.829935723940169No Hit
GTGCCCGAGTTGTAATTTG3674910.7790910996043074No Hit
AGAGGTGCACAATCGACCGATCCTGA3640720.7718427248970435No Hit
TAAGAGGTGCACAATCGACCGATCCTGA3353120.7108707282369352No Hit
AGGTGCTGCAATCT3202390.6789155507105855No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT3030090.6423874734347279No Hit
AAATCGACCGATCCTG2977410.6312191675096427No Hit
TAGATGGATACGAAT2768050.5868342675765401No Hit
TGTGCCCGAGTTGTAATTTG2764920.5861706989063519No Hit
AATCGACCGATCCTGA2759160.5849495629509895No Hit
ATGGAATAATAGAATA2609910.5533081495242816No Hit
TTTGCTCGAATATATT2476030.5249252186729072No Hit
ACAATCGACCGATCCTGA2474070.5245096932436519No Hit
AGCATTCAAGGTCCC2358040.49991101183728065No Hit
CAAGAGGTGCACAATCGACCGATCCTG2187870.46383450046158303No Hit
AGCATTCAAGGTCCCA2046800.4339272696937058No Hit
AGATGGATACGAATAAGGCGTC1988550.4215781083395636No Hit
TTTTCCACGTTCTAGC1969280.41749281496112023No Hit
AAATATAGATGGATACGAATAAGGCGTC1897680.4023134166271016No Hit
TGCCCGAGTTGTAATTTG1865980.3955929288172079No Hit
ATGCCTGTTTGAGCGTCATTT1860390.3944078333327503No Hit
TCACGTTCTAGCATTCAAGGTCCCA1821010.3860591642490401No Hit
ATAGATGGATACGAAT1812500.3842550206761002No Hit
TTGCTCGAATATATTAGC1702970.3610343572749121No Hit
TCACGTTCTAGCATTCAAGGTCCC1646800.34912616168242855No Hit
ATTAGCATGGAATAATAGAAT1619350.3433066856451546No Hit
TTGCCCGAGTTGTAATTTG1598030.33878678658815353No Hit
TTTCCACGTTCTAGC1430790.30333144332863854No Hit
TATAGATGGATACGAATAAGGCGTC1361670.2886778118642898No Hit
AAAGAGGTGCACAATCGACCGATCCTG1357070.2877025991221601No Hit
TTTGCTCGAATATATTAGC1335540.2831381794834531No Hit
AGAGGTGCACAATCGACCGATCCTG1303520.2763498507871504No Hit
ATATAGATGGATACGAATAAGGCGTC1303230.2762883699838422No Hit
TATAGATGGATACGAAT1266960.26859902951491965No Hit
AGCATGGAATAATAGAAT1256700.2664238810944304No Hit
AAGAGGTGCACAATCGACCGATC1226950.26011679868609167No Hit
ATATTAGCATGGAAT1183020.2508035169987531No Hit
TAAGAGGTGCACAATCGACCGATCCTG1170680.24818740281660523No Hit
CACGTTCTAGCATTCAAGGTCCCA1150410.24389010666813374No Hit
TTGCTCGAATATATT1135600.24075034564401618No Hit
TCTTCTTCATGATAAGACAGTT1135440.24071642520081168No Hit
ATATAGATGGATACGAAT1134630.24054470295708885No Hit
CACGTTCTAGCATTCAAGGTCCC1097360.2326433597181381No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA1041800.22086448581537166No Hit
ATGCCTGTTTGAGCGTC1040330.22055284174343023No Hit
AATCGACCGATCCTG1030000.21836285312903897No Hit
AAGAGGTGCACAATCGACCGATCCT1015500.21528881296363017No Hit
AAATATAGATGGATACGAAT997040.21137524182890974No Hit
TTTTGATGATTCAT988680.20960289867147405No Hit
AAATCGACCGATCCT977380.20720726737015546No Hit
ACGTTCTAGCATTC965540.20469715457302165No Hit
ATATTAGCATGGAATAATAGAAT951090.20163371454611426No Hit
TCACGTTCTAGCATTCAAGGTCC944780.20029597706723634No Hit
ACGTTCTAGCATTCAAGGTCCC936070.1984494329402908No Hit
AGCATTCAAGGTCC932050.19759718180477745No Hit
TATGGAATAATAGAAT910980.19313028344028343No Hit
TCACGTTCTAGCATTC862700.18289478970332226No Hit
ACGAATAAGGCGTCC858480.1820001380138033No Hit
AAGAGGTGCACAATCGACCGAT857110.18170969421886465No Hit
CCTGATTCAAGCTCAGGTA851380.1804949183466031No Hit
TAGGTGCTGCAATCT844310.1789960587625038No Hit
ACGTTCTAGCATTCAAGGTCCCA839710.1780208460203741No Hit
CACGTTCTAGCATTC816240.1730451410078124No Hit
AGAGGTGCACAATCGACCGATC811660.17207416832108327No Hit
TTTGCTCGAATATATTAGCATGGAAT811470.17203388779477793No Hit
TTTGACCTCAAATC805700.17081063181171524No Hit
AGTCAAGGATGCTGGC805060.1706749500388972No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC803640.17037390610545716No Hit
AGATGGATACGAATAAGGCGTCC802220.17007286217201714No Hit
TGTGCCCGAGTTGT777850.16490635466643008No Hit
TATATAGATGGATACGAATAAGGCGTC768680.16296228926527154No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA747970.15857171189798766No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC724590.1536150871347285No Hit
GTGCCCGAGTTGTAATTT722930.1532631625364817No Hit
TGGAATAATAGAAT703210.14908246791152574No Hit
TAGATGGATACGAATAAGGCGTC690820.14645575359087643No Hit
ACAATCGACCGATCCTG687630.14577946475448647No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA670610.14217117760860662No Hit
CACGTTCTAGCATTCAAGGTCC659590.13983490708289595No Hit
CCACGTTCTAGCATTCAAGGTCCCA656830.13924977943761813No Hit
GTGCCCGAGTTGTAATT653900.13862861132143553No Hit
AAGAGGTGCACAATC635840.13479984129472636No Hit
TTATAGATGGATACGAATAAGGCGTC631640.13390942966060795No Hit
CCACGTTCTAGCATTCAAGGTCCC608440.12899096539595387No Hit
TATTAGCATGGAATAATAGAAT580970.1231672493032794No Hit
ATGCCTGTTTGAGC574040.12169807010698401No Hit
ACGTTCTAGCATTCAAGGTCC569190.12066985667234728No Hit
AAGAGGTGCACAATCGACC567540.12032005210180076No Hit
ATCGACCGATCCTGA567350.12027977157549541No Hit
ATGTATTTATTAGAT544450.11542490814184977No Hit
TTCCACGTTCTAGC533330.11306743733913627No Hit
ACAATCGACCGATC520080.11025840063626272No Hit
TCAGATTTAGGCTCTGATA514980.10917718650911892No Hit
TTTTCCACGTTCTAGCATT506460.10737092290847873No Hit
AGTTATCGTTTATTTGATAGTT504400.10693419720222064No Hit
AGAGCTAATACATGCT501690.10635966969544425No Hit
ATAGATGGATACGAATAAGGCGTC490330.10395131822792396No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA485810.10299306570739654No Hit
TGTGCCCGAGTTGTAATTT483700.10254573986263704No Hit
TTTGTGGCGTCGCT482280.10224469592919701No Hit
ATTAATCAAGAACGA482060.10219805531979081No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAT481640.10210901415637896No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG478770.10150056620639805No Hit
TCAATATAGATGGATACGAATAAGGCGTC476090.10093239878272249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)