FastQCFastQC Report
Sun 2 Jul 2023
SRR3296804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22204693
Total Bases510.2 Mbp
Sequences flagged as poor quality0
Sequence length14-36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCC9019854.062136774419714No Hit
AAGAGGTGCACAATCGACCGATCCTGA6071992.73455255607452No Hit
TCACGTTCTAGCATTCAAGGTCCCA5390562.4276669801289303No Hit
CACGTTCTAGCATTCAAGGTCCC4024341.8123826346079182No Hit
AAGAGGTGCACAATCGACCGATCCTG3508941.5802695403174454No Hit
AAATATAGATGGATACGAATAAGGCGTC3053311.3750741791386172No Hit
TCACGTTCTAGCATTCAAGGTCC2770891.24788485028818No Hit
TGTGCCCGAGTTGTAATTTG2746231.2367790898977977No Hit
TATAGATGGATACGAATAAGGCGTC2518071.1340260367481776No Hit
ATATAGATGGATACGAATAAGGCGTC2518041.1340125260907683No Hit
GTGCCCGAGTTGTAATTTG2479771.1167774307890679No Hit
CACGTTCTAGCATTCAAGGTCCCA2315321.0427165104241702No Hit
AGTCAAGGATGCTGGC2069990.932230857684004No Hit
CCACGTTCTAGCATTCAAGGTCCC1890580.8514326228243732No Hit
ACGTTCTAGCATTCAAGGTCCC1772790.798385278283289No Hit
TTTGACCTCAAATC1760220.7927243128288239No Hit
TTGCCCGAGTTGTAATTTG1718290.7738409173231984No Hit
CAAGAGGTGCACAATCGACCGATCCTGA1515690.6825989442862371No Hit
TATATAGATGGATACGAATAAGGCGTC1490520.671263502719898No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1457500.6563927724648119No Hit
TTATAGATGGATACGAATAAGGCGTC1417650.6384461158728922No Hit
CACGTTCTAGCATTCAAGGTCC1303500.587038064430794No Hit
AAATATAGATGGATACGAATAAGGCGTCC1249780.5628449805633431No Hit
CCACGTTCTAGCATTCAAGGTCCCA1212990.5462764110271644No Hit
AGATGGATACGAATAAGGCGTC1192950.5372512918778026No Hit
TGCCCGAGTTGTAATTTG1165260.5247809550890886No Hit
TAGATGGATACGAATAAGGCGTC1145290.515787360807015No Hit
ACGTTCTAGCATTCAAGGTCCCA1135420.5113423545193803No Hit
AAAGAGGTGCACAATCGACCGATCCTGA1049720.4727469098536962No Hit
ATATAGATGGATACGAATAAGGCGTCC1026790.4624202640405792No Hit
CAAGAGGTGCACAATCGACCGATCCTG1025080.4616501565682534No Hit
TAAGAGGTGCACAATCGACCGATCCTGA985480.4438160887880773No Hit
AGAGGTGCACAATCGACCGATCCTGA958590.4317060361969427No Hit
TATAGATGGATACGAATAAGGCGTCC932110.41978062925706744No Hit
TTGCTCGAATATATTAGC930040.41884839389583095No Hit
TTTGCTCGAATATATTAGC927280.4176054134141823No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT801990.3611804045207921No Hit
ACAATCGACCGATCCTGA771050.34724641317941213No Hit
ATAGATGGATACGAATAAGGCGTC733720.33043465180986736No Hit
TCAATATAGATGGATACGAATAAGGCGTC698880.3147442750052883No Hit
AGTTATCGTTTATTTGATAGTT665400.299666381336594No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC665050.29950875700015306No Hit
AAAGAGGTGCACAATCGACCGATCCTG634600.28579543972979043No Hit
TATATAGATGGATACGAATAAGGCGTCC625740.28180529224159956No Hit
AAAATCAGTTATCGTTTATTTGATAGTT623260.28068841122910365No Hit
TAAGAGGTGCACAATCGACCGATCCTG601770.2710102769716294No Hit
ACGTTCTAGCATTCAAGGTCC594660.2678082511656432No Hit
TTAGATGGATACGAATAAGGCGTC586840.2642864731343054No Hit
CCACGTTCTAGCATTCAAGGTCC545000.24544360960090736No Hit
AGAGGTGCACAATCGACCGATCCTG541580.24390339465625577No Hit
AAAAGCGAATGATTAGAGGT521030.23464859433093718No Hit
AAATCGACCGATCCTGA517930.23325249306531734No Hit
TTATAGATGGATACGAATAAGGCGTCC512840.23096018485821895No Hit
AGATGGATACGAATAAGGCGTCC496370.22354283394055482No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC467580.2105771063801693No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC462940.20848745803420926No Hit
TTTAACGATCAACTTAGAACTGGT460350.20732103794454623No Hit
TTTTGATGATTCAT458800.20662298731173634No Hit
CTACATGGTATAACTGTGGT453130.20406947306139292No Hit
TAATATAGATGGATACGAATAAGGCGTC447960.20174113643453662No Hit
GCACGTTCTAGCATTCAAGGTCCC447920.20172312222465766No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC441320.19875077759462834No Hit
AAATTCTAGAGCTAATACATGCT422650.19034264513362106No Hit
AAATATAGATGGATACGAAT418060.18827551455000976No Hit
TTTGCTCGAATATATT416030.18736129339865226No Hit
ACGATCAACTTAGAACTGGT404200.18203359082694814No Hit
ACGAATAAGGCGTC403260.18161025689479246No Hit
TAGATGGATACGAATAAGGCGTCC398700.1795566369685904No Hit
AATATAGATGGATACGAATAAGGCGTC394920.17785429413502812No Hit
ATGGAGAACAGAAATCTCCAGT392160.17661131365337948No Hit
TCCACGTTCTAGCATTCAAGGTCCC389900.17559351079521793No Hit
AGTTGAACTTTGGG379350.17084226293964075No Hit
ATGATGCCCTGACGAGT373770.16832928066152503No Hit
ATTAGCATGGAATAATAGAAT364340.16408243068255884No Hit
ATGGAATAATAGAAT361040.16259625836754418No Hit
GTGCCCGAGTTGTAATTT357880.1611731357871059No Hit
ACTGATAACCGCAGC355260.15999320504003364No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA353310.15911501230843408No Hit
CAATATAGATGGATACGAATAAGGCGTC352520.1587592316633245No Hit
AAATGAAAGTGAACGT342110.1540710335423237No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC335350.1510266320727785No Hit
TATGATGCCCTGACGAGT335000.15086900773633752No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA333620.15024751749551324No Hit
TGTGCCCGAGTTGTAATTT329580.14842808229773768No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA327150.14733371904759054No Hit
AAATCGACCGATCCTG325250.14647804407833964No Hit
AGACACCACAAAAGGTGTTAGTTC320460.14432084244533352No Hit
ATATAGATGGATACGAAT320050.1441361967940741No Hit
ATATTAGCATGGAAT311560.14031268074726364No Hit
ACAATCGACCGATCCTG310820.13997941786450277No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA310600.13988033971016847No Hit
TCCACGTTCTAGCATTCAAGGTCCCA304680.13721423664808155No Hit
ATAGATGGATACGAATAAGGCGTCC296170.1333817134963316No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC290780.13095429871514097No Hit
TTGGAATGAGTACAATGTAAATACCTT289610.13042738307618124No Hit
CCTGATTCAAGCTCAGGT289350.13031029071196798No Hit
ATGTCTAAGTATAAGCAA288850.1300851130884809No Hit
GTTTTCATGCCAGGTCGT284130.12795943632276294No Hit
AGCATGGAATAATAGAAT278190.12528432615573654No Hit
AATAGATGGATACGAATAAGGCGTC275820.12421698422040782No Hit
TCAATATAGATGGATACGAATAAGGCGTCC273930.1233658128036267No Hit
TTTTCCACGTTCTAGCATTCAAGGTC272480.1227127976955142No Hit
ATGGAATAATAGAATA266940.12021782962727745No Hit
TATTTGATCAGACA266510.12002417687107857No Hit
ACACGTTCTAGCATTCAAGGTCCC265410.11952878609940701No Hit
ACCCGCTGAACTTAAGC263850.11882623191412733No Hit
GCACGTTCTAGCATTCAAGGTCCCA260040.11711037842315586No Hit
TTTCCACGTTCTAGCATTCAAGGTCC259350.11679963330274372No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG253880.11433619010179515No Hit
AGGTGCTGCAATCT252850.11387232419741178No Hit
TATGACTCTTAATCATA250190.11267437924046056No Hit
TTTGACCTCAAATCAGGT248160.11176015808910306No Hit
TTTGTGGCGTCGCTGAACCATA247750.11157551243784365No Hit
TCTGACTTCTAATCAGA245560.11058923444697027No Hit
ATGTCTAAGTATAAGCAAT245050.11035955327101346No Hit
AAGAGGTGCACAATCGACCGATC244690.1101974253821028No Hit
AGTCAAGGATGCTGGCAT244570.11014338275246588No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG235930.10625231341860929No Hit
AAGAGGTGCACAATCGACCGATCCT229870.103523160621946No Hit
AAGCGGAGGAAAAGAAACC228350.10283862064654531No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)