FastQCFastQC Report
Sun 2 Jul 2023
SRR3296805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30064532
Total Bases689.9 Mbp
Sequences flagged as poor quality0
Sequence length14-36
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA13745804.572098444772066No Hit
TCACGTTCTAGCATTCAAGGTCCC10308543.428804413120417No Hit
AAGAGGTGCACAATCGACCGATCCTG8386882.7896259951759768No Hit
TCACGTTCTAGCATTCAAGGTCCCA5425401.8045848842749326No Hit
CACGTTCTAGCATTCAAGGTCCC5197781.7288744092208055No Hit
ACGTTCTAGCATTCAAGGTCCC3496371.16295507277479No Hit
TCACGTTCTAGCATTCAAGGTCC3114801.0360380796880524No Hit
CAAGAGGTGCACAATCGACCGATCCTGA3039141.0108722131447117No Hit
AAATATAGATGGATACGAATAAGGCGTC2953250.9823036659942022No Hit
CACGTTCTAGCATTCAAGGTCCCA2599250.8645569470364614No Hit
TATAGATGGATACGAATAAGGCGTC2468770.821156969947179No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2400590.7984790849230582No Hit
CCACGTTCTAGCATTCAAGGTCCC2256880.7506785736761178No Hit
TAAGAGGTGCACAATCGACCGATCCTGA2226880.7407000381712244No Hit
ATATAGATGGATACGAATAAGGCGTC2144840.713412069743843No Hit
ACGTTCTAGCATTCAAGGTCCCA2134660.7100260200291826No Hit
AGAGGTGCACAATCGACCGATCCTGA2112220.7025620754715224No Hit
ACGAATAAGGCGTC2050990.6821958845060352No Hit
CAAGAGGTGCACAATCGACCGATCCTG1885120.6270245616994803No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT1680580.5589909066271179No Hit
GTGCCCGAGTTGTAATTTG1668420.5549462735691345No Hit
AAATCGACCGATCCTGA1664150.5535259953489381No Hit
CACGTTCTAGCATTCAAGGTCC1631680.5427258937541419No Hit
ATGGAATAATAGAAT1621370.5392966037189603No Hit
AAAGAGGTGCACAATCGACCGATCCTG1531090.5092678642062347No Hit
AGATGGATACGAATAAGGCGTC1523290.5066734449749626No Hit
TGTGCCCGAGTTGTAATTTG1515200.503982566567143No Hit
AGTCAAGGATGCTGGC1488180.4949952322557357No Hit
TAAGAGGTGCACAATCGACCGATCCTG1422720.4732220677840586No Hit
TTATAGATGGATACGAATAAGGCGTC1362330.4531352758127085No Hit
AGAGGTGCACAATCGACCGATCCTG1354290.4504610282973971No Hit
CCACGTTCTAGCATTCAAGGTCCCA1349680.4489276600081451No Hit
ATTAGCATGGAATAATAGAAT1346720.44794311117166236No Hit
TATATAGATGGATACGAATAAGGCGTC1280740.42599698541790043No Hit
TAGATGGATACGAATAAGGCGTC1265850.4210443056289717No Hit
TTTGCTCGAATATATT1265240.4208414087403722No Hit
TTTGACCTCAAATC1252870.4167269259338546No Hit
ACGTTCTAGCATTCAAGGTCC1241540.4129583656915065No Hit
AAATCGACCGATCCTG1209930.40244431544785064No Hit
TTTTGATGATTCAT1032970.34358426068298686No Hit
TTTGCTCGAATATATTAGC997130.331663236933141No Hit
AAATATAGATGGATACGAATAAGGCGTCC966040.32132214797156994No Hit
TTGCTCGAATATATTAGC903330.30046368258784134No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT882400.29350199098392754No Hit
ATAGATGGATACGAATAAGGCGTC833260.2771571498269123No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC807660.2686421328627367No Hit
ATGGAATAATAGAATA790480.26292775819693454No Hit
AAGAGGTGCACAATCGACCGATC762930.2537641364249409No Hit
TATAGATGGATACGAATAAGGCGTCC741400.2466028741109291No Hit
ATATAGATGGATACGAATAAGGCGTCC710240.23623850189984663No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC691460.22999193867378345No Hit
CCACGTTCTAGCATTCAAGGTCC680370.22630320671547458No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC666250.22160664267117147No Hit
ATATTAGCATGGAAT651960.21685353359234064No Hit
AGAGGTGCACAATCGACCGATC645010.21454183953370703No Hit
TTAGATGGATACGAATAAGGCGTC625350.20800257259950028No Hit
ACAATCGACCGATCCTGA613680.2041209222880968No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA611170.2032860514841874No Hit
TCAATATAGATGGATACGAATAAGGCGTC608360.20235139532522906No Hit
AGATGGATACGAATAAGGCGTCC598360.199025216823598No Hit
AGCATGGAATAATAGAAT591620.19678337251349864No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC589980.19623787923923114No Hit
AATCGACCGATCCTGA586670.19513691415519124No Hit
GCACGTTCTAGCATTCAAGGTCCC579230.1926622373499777No Hit
ATATTAGCATGGAATAATAGAAT577040.1919338042581205No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA575550.19143820366137745No Hit
AAAAGCGAATGATTAGAGGT571630.19013434168873808No Hit
TGCCCGAGTTGTAATTTG560540.1864456097304292No Hit
AAGAGGTGCACAATCGACCGATCCT543960.18093080577472484No Hit
TTGCCCGAGTTGTAATTTG543430.1807545183141384No Hit
AGTTATCGTTTATTTGATAGTT527530.17546589449654498No Hit
TCCACGTTCTAGCATTCAAGGTCCC526230.17503349129133292No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA519790.1728914323362825No Hit
AGCATTCAAGGTCCC500780.1665683670046818No Hit
AGCATTCAAGGTCCCA493970.16430323944507103No Hit
AAATTCTAGAGCTAATACATGCT491400.16344841157015183No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA484270.16107684629848884No Hit
AGGTGCTGCAATCT471440.15680935928089618No Hit
AAATATAGATGGATACGAAT468640.15587802930043948No Hit
ATTAATCAAGAACGA468510.15583478897991826No Hit
ACGAATAAGGCGTCC456460.1518267438854528No Hit
TTTGCTCGAATATATTAGCATGGAAT451950.15032663738121718No Hit
TATTAGCATGGAATAATAGAAT446490.1485105439193266No Hit
AGACACCACAAAAGGTGTTAGTTC444030.14769230400792535No Hit
TATATAGATGGATACGAATAAGGCGTCC439500.14618554514668647No Hit
ACAATCGACCGATCCTG439100.1460524980066212No Hit
TAATATAGATGGATACGAATAAGGCGTC429990.1430223493916353No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA425790.14162535442095026No Hit
TTGCTCGAATATATT425670.14158544027893066No Hit
ATTAGCATGGAATAATAGAATA425150.14141247899684586No Hit
AATCGACCGATCCTG424620.1412361915362594No Hit
CTACATGGTATAACTGTGGT413620.1375773951844652No Hit
TTATAGATGGATACGAATAAGGCGTCC413050.13738780300987224No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC413020.13737782447436733No Hit
TTTCCACGTTCTAGCATTCAAGGTCC412310.13714166580075154No Hit
AAAATCAGTTATCGTTTATTTGATAGTT411990.13703522808869933No Hit
TTTTCCACGTTCTAGCATTCAAGGTC405670.1349330832756685No Hit
TTTTCCACGTTCTAGC394300.13115121831931395No Hit
TCCACGTTCTAGCATTCAAGGTCCCA392580.13057911561703336No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC392170.1304427422984665No Hit
TCAAGAGGTGCACAATCGACCGATCCTG381930.12703673551279626No Hit
TAGATGGATACGAATAAGGCGTCC378780.1259889892847825No Hit
AATATAGATGGATACGAATAAGGCGTC375830.1250077666268013No Hit
AAATGAAAGTGAACGT366380.12186452794275993No Hit
TATTTGATCAGACA357490.11890755525480988No Hit
ATGATGCCCTGACGAGT355020.11808598916490702No Hit
TTTGTGGCGTCGCTGAACCATA343650.11430412420855246No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG335190.11149017719617256No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG328950.10941464181115476No Hit
ATTCAATGAAGCGGAG326980.10875938464633343No Hit
AGTTGAACTTTGGG319180.10616496541506118No Hit
ACACGTTCTAGCATTCAAGGTCCC315770.10503073854600499No Hit
ATTAATCAAGAACG315190.10483782019291037No Hit
CAATATAGATGGATACGAATAAGGCGTC308850.10272902302287626No Hit
TATGACTCTTAATCATA303770.10103932434404767No Hit
GCACGTTCTAGCATTCAAGGTCCCA302860.10073664210039923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)