FastQCFastQC Report
Sun 2 Jul 2023
SRR3296806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3296806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41845273
Total Bases964.2 Mbp
Sequences flagged as poor quality0
Sequence length14-35
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGA15191783.6304650228951787No Hit
TCACGTTCTAGCATTCAAGGTCCC11379802.719494744364555No Hit
AAGAGGTGCACAATCGACCGATCCTG7468681.7848324230074923No Hit
TCACGTTCTAGCATTCAAGGTCCCA6633951.5853523049066978No Hit
CACGTTCTAGCATTCAAGGTCCC5519431.3190091984822276No Hit
TCACGTTCTAGCATTCAAGGTCC4741431.133086167223715No Hit
GTGCCCGAGTTGTAATTTG4620381.1041581685940967No Hit
AAATATAGATGGATACGAATAAGGCGTC4248811.015361997996763No Hit
CAAGAGGTGCACAATCGACCGATCCTGA4210811.006280924490563No Hit
TGTGCCCGAGTTGTAATTTG4201561.004070400018659No Hit
TATAGATGGATACGAATAAGGCGTC3510170.8388450470857246No Hit
ATATAGATGGATACGAATAAGGCGTC3194720.7634601882033366No Hit
CACGTTCTAGCATTCAAGGTCCCA3053900.7298076415943087No Hit
ACGAATAAGGCGTC2915000.6966139281729624No Hit
CCACGTTCTAGCATTCAAGGTCCC2704260.6462522063125267No Hit
TTGCCCGAGTTGTAATTTG2688610.6425122378816839No Hit
AAAGAGGTGCACAATCGACCGATCCTGA2637470.6302910247472875No Hit
ACGTTCTAGCATTCAAGGTCCC2560980.6120117796817816No Hit
TAAGAGGTGCACAATCGACCGATCCTGA2460330.5879588836712811No Hit
CAAGAGGTGCACAATCGACCGATCCTG2458360.5874881017026702No Hit
CACGTTCTAGCATTCAAGGTCC2403840.5744591509774593No Hit
TTTGCTCGAATATATT2306360.5511638076778708No Hit
AGAGGTGCACAATCGACCGATCCTGA2283230.5456363016200182No Hit
TTATAGATGGATACGAATAAGGCGTC2139760.511350469621742No Hit
TGCCCGAGTTGTAATTTG2022740.4833855427350181No Hit
AGATGGATACGAATAAGGCGTC2019480.4826064822184336No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAT1987990.47508113998921697No Hit
TATATAGATGGATACGAATAAGGCGTC1912130.456952449563419No Hit
TTTGCTCGAATATATTAGC1883660.4501488137023267No Hit
TAGATGGATACGAATAAGGCGTC1834190.4383266898509659No Hit
TTGCTCGAATATATTAGC1827010.43661084490953134No Hit
AAATATAGATGGATACGAATAAGGCGTCC1713620.4095133995182682No Hit
CCACGTTCTAGCATTCAAGGTCCCA1675280.40035107430174965No Hit
ATGGAATAATAGAAT1519180.36304698024075505No Hit
ACGTTCTAGCATTCAAGGTCCCA1502000.35894137911347834No Hit
AAAGAGGTGCACAATCGACCGATCCTG1369570.32729383794437183No Hit
ATATTAGCATGGAAT1363860.32592928716225605No Hit
AGTCAAGGATGCTGGC1361840.32544655641271597No Hit
TATAGATGGATACGAATAAGGCGTCC1340470.32033964744357146No Hit
ATATAGATGGATACGAATAAGGCGTCC1317050.31474283845632933No Hit
TAAGAGGTGCACAATCGACCGATCCTG1277140.30520532151863367No Hit
TTTGACCTCAAATC1212270.2897029731410762No Hit
AGAGGTGCACAATCGACCGATCCTG1208300.28875423993529686No Hit
AGACACCACAAAAGGTGTTAGTTC1201840.2872104574392429No Hit
TTTTCCACGTTCTAGC1184630.2830976870434087No Hit
TCAATATAGATGGATACGAATAAGGCGTC1171800.28003162985697333No Hit
TTTTGATGATTCAT1148600.27448739550581974No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCC1147710.2742747072052798No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1125630.2689981255469405No Hit
AGTTATCGTTTATTTGATAGTT1119950.2676407440333822No Hit
ACGTTCTAGCATTCAAGGTCC1118420.26727511133694837No Hit
TTTGCTCGAATATATTAGCATGGAAT1107990.2647825956351151No Hit
CCACGTTCTAGCATTCAAGGTCC1053010.2516437161253554No Hit
TTTTCCACGTTCTAGCATTCAAGGTCC1013190.24212770699333233No Hit
ATGGAATAATAGAATA1006220.24046204693180037No Hit
CTACATGGTATAACTGTGGT942830.22531338246974753No Hit
TTTCCACGTTCTAGCATTCAAGGTCCC940890.22484976976969417No Hit
AAGAGGTGCACAATCGACCGATC939720.22457016829595067No Hit
AGATGGATACGAATAAGGCGTCC937240.22397750876186184No Hit
AAATCGACCGATCCTGA935680.22360470679687044No Hit
TTAGATGGATACGAATAAGGCGTC918280.21944653103350528No Hit
ATAGATGGATACGAATAAGGCGTC910680.2176303163322653No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCA893140.21343868398229832No Hit
TCAAGAGGTGCACAATCGACCGATCCTGA855680.2044866573101339No Hit
TATATAGATGGATACGAATAAGGCGTCC823370.19676535507367823No Hit
TTTCCACGTTCTAGC812020.19405298180274747No Hit
TTATAGATGGATACGAATAAGGCGTCC788690.18847768062117792No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCA787750.1882530435397088No Hit
ATGGAGAACAGAAATCTCCAGT783890.18733059765197374No Hit
TTGCTCGAATATATT768210.18358345995257336No Hit
ACAATCGACCGATCCTGA767730.18346875165565296No Hit
TTTTCTTTACTTATTCAATGAAGCGGAG741350.17716457483740158No Hit
TCCACGTTCTAGCATTCAAGGTCCC737140.1761584874831621No Hit
TTTTCCACGTTCTAGCATTCAAGGTC724660.1731760717632312No Hit
ATTAGCATGGAATAATAGAAT708020.16919951746998999No Hit
AGCATGGAATAATAGAAT705590.16861880671683036No Hit
TAGATGGATACGAATAAGGCGTCC698010.16680737152796207No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCA693250.16566984758350126No Hit
TTTCCACGTTCTAGCATTCAAGGTCC683140.16325380407961493No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGC669240.15993204298129446No Hit
AGAGGTGCACAATCGACCGATC668030.15964288248280756No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAG655160.1565672662716288No Hit
CCTGATTCAAGCTCAGGTA652850.1560152325926993No Hit
AAATATAGATGGATACGAAT650630.15548470671944237No Hit
ACTTTTACTTTGAAAAAATTAGAGTGTTC638280.1525333578299274No Hit
AAATCGACCGATCCTG623990.1491183962403591No Hit
AAGAGGTGCACAATCGACCGAT618700.14785421521804865No Hit
TAATATAGATGGATACGAATAAGGCGTC614420.14683139957050823No Hit
AGATGGATACGAAT602210.14391350726759508No Hit
GCACGTTCTAGCATTCAAGGTCCC586770.14022372371665492No Hit
CAATATAGATGGATACGAATAAGGCGTC578610.1382736826690078No Hit
CCTGATTCAAGCTCAGGT571790.1366438689502635No Hit
AAGAGGTGCACAATCGACCGATCCT566920.13548005768775842No Hit
AATATAGATGGATACGAATAAGGCGTC534900.12782805838069214No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAA534020.12761775983633802No Hit
TCCACGTTCTAGCATTCAAGGTCCCA528010.12618151636864694No Hit
AAAATCAGTTATCGTTTATTTGATAGTT520340.12434857337410608No Hit
TCAAGAGGTGCACAATCGACCGATCCTG517640.12370333920392872No Hit
AGCATTCAAGGTCCCA516250.12337116309409668No Hit
ATTCAATGAAGCGGAG516020.1233161987018223No Hit
TTTCTTTACTTATTCAATGAAGCGGAG501290.11979608784007695No Hit
TCTTCTTCATGATAAGACAGTT478690.11439523886007388No Hit
AGCATTCAAGGTCCC477450.11409890909302946No Hit
TCAATATAGATGGATACGAATAAGGCGTCC471140.11259097293976311No Hit
ATATAGATGGATACGAAT462400.11050232603333715No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC457390.10930505818423028No Hit
TAGATGGATACGAAT454230.10854989522950417No Hit
AGAGCTAATACATGCT451270.10784252739849494No Hit
AGGTGCTGCAATCT444020.10610995416375944No Hit
TCAGATTTAGGCTCTGATA441180.1054312634069803No Hit
TTTGTGGCGTCGCTGAACCATAGC434760.10389703993566968No Hit
ACAATCGACCGATCCTG434350.10379905993205016No Hit
TTTGTGGCGTCGCTGAACCATA432070.10325419552167817No Hit
TTTCCACGTTCTAGCATTCAAGGTC426110.10182990083491628No Hit
ATAGATGGATACGAAT424180.10136867789104877No Hit
GTGCCCGAGTTGTAATTT420470.10048207834610136No Hit
TCACGTTCTAGCATTC419940.10035542126825173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)