FastQCFastQC Report
Sun 2 Jul 2023
SRR3306576.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306576.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66669156
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA9078061.3616581556844667No Hit
ACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGA3752730.5628884817440917No Hit
GTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA3531700.5297352196868969No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCCCAAAGATTAAGCCA3050440.45754891512350926No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG2499350.37488850166334786No Hit
CTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAG2411260.3616754950370153No Hit
AGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAG2335620.35032991868083646No Hit
AACAGCTAAGGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATCA2258270.33872785190201No Hit
TACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAG2211150.33166011581127564No Hit
AGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG2134060.3200970475762435No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC2036990.30553709124501294No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA2028020.304191641484107No Hit
TAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGA2021520.3032166778892476No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC1727930.2591798222254381No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1627550.24412338443282527No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1602730.24040052344445456No Hit
GTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAG1571820.23576419656490027No Hit
CTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACG1570620.2355842032858493No Hit
AGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCAC1482010.22229319957192797No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA1478520.22176971911868812No Hit
TCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCAC1461880.21927381231584814No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC1443740.21655291391419443No Hit
TAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC1299970.1949882191398973No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG1290290.1935362733555529No Hit
TGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGG1259560.1889269454678562No Hit
TAACAGCTAAGGACTGCAAAACCCCACTCTGCATCAACTGAACGCAAATC1256520.18847096249426046No Hit
TCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCA1191160.1786673285619515No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA1188940.17833434099570722No Hit
ATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA1172010.1757949358170966No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1135690.17034713923782085No Hit
GAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGG1120860.1681227222975494No Hit
TGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGA1021830.15326877694386892No Hit
TTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAA944580.14168170960496335No Hit
TTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC839430.12590979852812298No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA818290.12273891692884187No Hit
TACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAA796120.11941354109837538No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA731950.10978840050112529No Hit
AGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGT726300.1089409321455937No Hit
ATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACC690600.10358613209382761No Hit
GTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACG686610.10298765444098318No Hit
CGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCAC683680.10254817085130041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)