FastQCFastQC Report
Thu 6 Jul 2023
SRR3306583.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306583.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178511892
Total Bases9.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2237494912.534150385902581No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG126032847.060192942215861Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG40258952.2552531122128268No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG34680981.9427826130485468Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAG26019641.457585806104167No Hit
GTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG25093081.4056811408396253Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA21213291.1883404384061986No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA20669661.1578870050853531RNA PCR Primer, Index 1 (100% over 22bp)
CTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGG17505730.9806478326945299Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA17224250.9648796955219096No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG14987630.8395872024032999No Hit
TGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA11973820.6707575537880692RNA PCR Primer, Index 1 (100% over 22bp)
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA11732460.657236885932507RNA PCR Primer, Index 1 (100% over 22bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG10362840.580512585682527Illumina Small RNA Adapter 2 (100% over 21bp)
ACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGA9218230.5163930479208634RNA PCR Primer, Index 1 (100% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGG7799240.4369031055925394Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA6215880.34820537334285834RNA PCR Primer, Index 1 (100% over 22bp)
TTGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG6209380.34784125194303583Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA5520600.3092567076707696RNA PCR Primer, Index 1 (100% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGA5290510.29636737030382265RNA PCR Primer, Index 1 (100% over 22bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAG5152290.2886244687832898No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG4866160.2725958447631041Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA4370550.24483242830679314No Hit
CCTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTC4356920.24406889374070387RNA PCR Primer, Index 1 (100% over 26bp)
CTTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTC4267450.23905690271883961RNA PCR Primer, Index 1 (100% over 26bp)
GTGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA4151390.23255537507831692RNA PCR Primer, Index 1 (100% over 22bp)
TAAATCAGTTATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAGGA3681120.2062114718945447RNA PCR Primer, Index 1 (100% over 22bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGA3643080.20408052142542976RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3526310.19753922052431105No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAA3338310.1870077092679069No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG3221150.18044456108279888No Hit
TGTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3204910.17953481777001165No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGGAATTCTCGGGTGCCAAG3077490.17239691795995304No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3017960.16906212612434807No Hit
TGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG3009480.16858708774427195Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGTGGAATTCTCGGGTGCCAAGGA2913430.16320649382843358RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA2862900.1603758700848905RNA PCR Primer, Index 1 (100% over 22bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAAG2836370.1588896945868458No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTGGAATTCTCGGGTGCCAAGG2813430.1576046261388569Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA2788200.15619127492077672RNA PCR Primer, Index 1 (100% over 23bp)
CCCGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGG2720970.1524251392730743Illumina Small RNA Adapter 2 (100% over 21bp)
ATGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2590420.14511190100433197No Hit
CGCCTGGATACCGCAGCTAGGAATAATGGATGGAATTCTCGGGTGCCAAG2523710.14137489506861536No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG2513570.1408068656848923Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG2420600.13559880929389287No Hit
GCATTTATCAGTTGGTGACTCTAGATAACCTTGGAATTCTCGGGTGCCAA2382920.13348802554846037No Hit
GTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2289580.12825924224700952No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA2110760.11824198244450852RNA PCR Primer, Index 1 (100% over 22bp)
TGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAAGG2104560.11789466664775476Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2094710.11734288268033147Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCTGGAATTCTCGGGTGCCA1922120.10767461923489108No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG1919690.10753849385003439No Hit
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA1840760.1031169396826515RNA PCR Primer, Index 1 (100% over 23bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG1822550.1020968395763796Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGGGCCAAG1801510.10091820661449268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)