FastQCFastQC Report
Tue 29 Aug 2023
SRR3306584.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306584.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242256008
Total Bases12.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT225006299.28795499676524RNA PCR Primer, Index 37 (96% over 33bp)
CATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG106919694.413500035879399RNA PCR Primer, Index 1 (100% over 29bp)
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTC73703343.042374082214712RNA PCR Primer, Index 37 (96% over 32bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC72588462.996353345342007RNA PCR Primer, Index 37 (97% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG55135882.275934473418715No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA54445002.2474158824577017No Hit
AGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAA47059601.942556570155321RNA PCR Primer, Index 37 (97% over 35bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC41528241.7142295187164152RNA PCR Primer, Index 1 (100% over 24bp)
AGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAAG40841401.6858776934853148No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG32349921.3353608963951886No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA21931830.9053162471000513RNA PCR Primer, Index 1 (100% over 23bp)
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA20834170.8600063285117784RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG19209090.7929252264406174Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG18829660.7772628697819539Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACT14615480.6033072253052234RNA PCR Primer, Index 37 (96% over 33bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG14582430.6019429660543238No Hit
GGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCC14331830.5915985373621776RNA PCR Primer, Index 37 (96% over 31bp)
AGTTCAACTTTAAATTTGCCCACAGAACCTGGAATTCTCGGGTGCCAAGG11572350.4776909392480371Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG11566640.4774552381792736No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA10999490.45404405409008475RNA PCR Primer, Index 1 (100% over 22bp)
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA9900500.40867923490260766RNA PCR Primer, Index 1 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA9553060.39433738213006464RNA PCR Primer, Index 1 (100% over 23bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTTGGAATTCTCGGGTGCCAAGG8881720.3666253759122457Illumina Small RNA Adapter 2 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAGG8343960.34442737122953004Illumina Small RNA Adapter 2 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGGAATTCTCGGGTGCCAAGG8189220.3380399135446829Illumina Small RNA Adapter 2 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGTGGAATTCTCGGGTGCCAAG7953220.3282981530844015No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA7835050.32342025548443776RNA PCR Primer, Index 1 (100% over 23bp)
ACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGGA7690560.31745590392127654RNA PCR Primer, Index 1 (100% over 22bp)
TAGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAA7548040.31157287129077105No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG7098350.2930102769628731Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG7009090.2893257450192938No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA6691940.2762342224346403No Hit
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACTC6618170.27318909671788205RNA PCR Primer, Index 37 (96% over 32bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA6392510.26387415745742826No Hit
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA6372220.26303661372972015RNA PCR Primer, Index 1 (100% over 22bp)
ATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT6289440.25961956741233844RNA PCR Primer, Index 1 (100% over 30bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTGGAATTCTCGGGTGCCA6206920.25621325354292146No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGG6017210.24838228160681983Illumina Small RNA Adapter 2 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGTGGAATTCTCGGGTGCCAAGGAA5824010.24040724719611495RNA PCR Primer, Index 1 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA5731250.23657823999147215RNA PCR Primer, Index 1 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA5627580.23229888275877147RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG5287130.21824556772189527No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA5220080.21547783450637886RNA PCR Primer, Index 1 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGTGGAATTCTCGGGTGCCAAGGA5147970.2125012313420107RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTATCCGGCTCGGAGGTGGAATTCTCGGGTGCCAAGGA4737560.19556006222970537RNA PCR Primer, Index 1 (100% over 22bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTGGAATTCTCGGGTGCCAAGGA4730390.19526409433775527RNA PCR Primer, Index 1 (100% over 22bp)
GCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA4655320.19216530638117343RNA PCR Primer, Index 37 (96% over 30bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG4635310.19133932067435042No Hit
AGTTCAACTTTAAATTTGCCCACAGAACTGGAATTCTCGGGTGCCAAGGA4529380.1869666737016487RNA PCR Primer, Index 1 (100% over 22bp)
GTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAAGG4324510.1785099174919121Illumina Small RNA Adapter 2 (100% over 21bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAAC4239930.17501856961169773RNA PCR Primer, Index 37 (97% over 34bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAGGA4220970.17423592648319375RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACCCTTGGAATTCTCGGGTGCCAA4180600.17256950754344139No Hit
AGTTCAACTTTAAATTTGCCCACAGAATGGAATTCTCGGGTGCCAAGGAA4118980.17002591737580353RNA PCR Primer, Index 1 (100% over 23bp)
CAGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAG3887690.16047857933826765No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG3757170.15509089045997984Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTG3564430.1471348442264433RNA PCR Primer, Index 37 (97% over 43bp)
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA3324570.13723374819253192RNA PCR Primer, Index 1 (100% over 23bp)
AGTGCGGTAACGCGACCGATCCCGGAGATGGAATTCTCGGGTGCCAAGGA3197330.13198145327318364RNA PCR Primer, Index 1 (100% over 22bp)
CACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC3165550.1306696179027271RNA PCR Primer, Index 37 (96% over 30bp)
GGTCGCTGGTTCGAATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA2839050.11719213997780396RNA PCR Primer, Index 1 (100% over 22bp)
TCTTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGG2791630.11523470658362372Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGGA2659200.10976817549144127RNA PCR Primer, Index 1 (100% over 22bp)
CCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA2609010.10769640024779076RNA PCR Primer, Index 1 (100% over 28bp)
CCGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT2606910.10760971509115266RNA PCR Primer, Index 37 (96% over 29bp)
GGTCGCTGGTTCGGATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA2579190.10646547102352978RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAA2549080.10522257099192356No Hit
GTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAGG2532780.10454972906182784Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2471630.10202553985781851RNA PCR Primer, Index 1 (100% over 33bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTGGAATTCTCGGGTGCCAAGGA2463600.10169407233029285RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)