FastQCFastQC Report
Mon 3 Jul 2023
SRR3306585.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306585.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116546351
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2319034219.897956307529522No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA43554313.7370805371675684No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG30762752.6395292290189336Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG29570442.5372257257543827No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAG19752441.694814108766048No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG13634251.169856446213404Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG12956001.1116607160013101No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG11000900.9439077161669353No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGG10792680.9260418629494458Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA9672570.8299333198342692No Hit
TTTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG8655350.7426530239458119No Hit
TGGTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG7000530.6006648805332395No Hit
TTGTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG6963630.5974987582408308No Hit
GTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG6121640.5252536821165683Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGG5605470.48096486521487064Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA5044980.4328732694513962RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA4676400.40124808369161213No Hit
ACTTTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAG4283640.3675481869011927No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG4251560.3647956339705565Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGACTGGAATTCTCGGGTGCCAA4164530.35732821871016796No Hit
CTTTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGG3736860.3206329471439222Illumina Small RNA Adapter 2 (100% over 21bp)
TTTTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG3712380.3185324952816412No Hit
GTTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG3519100.3019485354801027Illumina Small RNA Adapter 2 (100% over 21bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAG2932090.25158145019915723No Hit
TGTTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2928310.2512571157204227No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGGAATTCTCGGGTGCCAAG2911610.2498242094254843No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGGGCCAAG2789490.23934597489028206No Hit
GGTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2674260.2294589214552071Illumina Small RNA Adapter 2 (100% over 21bp)
ACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGA2563870.21998715343734787RNA PCR Primer, Index 1 (100% over 22bp)
GTTTGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2544590.2183328759902573Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCATGGAATTCTCGGGTGCCAAG2540930.218018837844181No Hit
TGGGGGGCCCAAGTCCTTCTTATCGAGGCCTGGAATTCTCGGGTGCCAAG2492300.21384624903442923No Hit
GTGTGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG2309240.19813919356428414Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCACGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2212750.18986008407933766No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA2162210.1855236119747756RNA PCR Primer, Index 1 (100% over 22bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2136110.1832841596216084No Hit
TTGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG2042840.17528133506299137Illumina Small RNA Adapter 2 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGA1990950.1708290292160241RNA PCR Primer, Index 1 (100% over 22bp)
NGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1839560.15783934753993284No Hit
GTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1823470.15645878093600718No Hit
AGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1770520.15191552415055878No Hit
GGGTGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1722970.14783560233473117Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGCTCGAGGCCTGGAATTCTCGGGTGCCAAG1672840.14353430936675143No Hit
GTTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1663570.1427389176689024No Hit
TGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG1602910.13753412151016206Illumina Small RNA Adapter 2 (100% over 21bp)
GTTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA1577730.13537360770737472No Hit
ATGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1507420.12934081479736761No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAGGA1500410.1287393373645821RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA1480950.1270696154185042RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA1419840.12182620801229546RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGTCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1381520.11853824578343082No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTATGGAATTCTCGGGTGCCAA1381290.11851851114583586No Hit
TTGGGGGCCCAAGTCCTTCTTATCGAGGCCTGGAATTCTCGGGTGCCAAG1315070.11283665157393044No Hit
CCTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTC1295920.11119352848721964RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCCCAAGTCCTTCTGATCGCGGCCTGGAATTCTCGGGTGCCAAG1236380.10608483143328958No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG1221770.10483125293214886No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTACCAAG1199980.10296161052695676No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAA1196930.10269991207189318No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTCTGGAATTCTCGGGTGCCAAGG1194450.10248712119695623Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG1182710.10147979665189175No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAA1168230.10023737251113078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)