FastQCFastQC Report
Thu 6 Jul 2023
SRR3306586.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306586.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239897608
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA77566043.233297765936874RNA PCR Primer, Index 1 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA63233602.6358578781660884RNA PCR Primer, Index 1 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA60041652.5028031959368264RNA PCR Primer, Index 1 (100% over 23bp)
CATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG52575322.191573331569025RNA PCR Primer, Index 1 (100% over 29bp)
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT51163092.132705299837754RNA PCR Primer, Index 37 (96% over 33bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA42140201.7565910869774077RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA39129401.6310875429820877No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG37323421.555806258810217Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA35364101.4741330809767808No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG31978991.3330266302613571No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA31802971.3256893332592128No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG24958231.0403701065664648Illumina Small RNA Adapter 2 (100% over 21bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG24668741.0283028749498828Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG24646161.0273616400543686Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG21968120.9157290138549443No Hit
TCTTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGG18140560.7561792779526172Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG17805150.7421978963625181Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG15812280.6591262052100161No Hit
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTC14156880.5901217656159373RNA PCR Primer, Index 37 (96% over 32bp)
CGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGGA12185990.5079662986885639RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG11467580.4780197725022752Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGGA11278890.47015433351048674RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA11002400.458628999752261RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG10037120.41839183323578616No Hit
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGG9952360.41485865919930304Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA9735290.4058102154982721RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG9292390.38734817230857926No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG9257510.3858942186701586No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA9198990.3834548446185424RNA PCR Primer, Index 1 (100% over 23bp)
AGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAA8937250.3725443565072979RNA PCR Primer, Index 37 (97% over 35bp)
CTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGA8714470.36325789459309654RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA8469240.35303561676196454RNA PCR Primer, Index 1 (100% over 22bp)
ATGTGTGACTGAAAGGTATTTTCTGAGCTGGAATTCTCGGGTGCCAAGGA7968640.33216838077018257RNA PCR Primer, Index 1 (100% over 22bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC7516320.31331367005543465RNA PCR Primer, Index 37 (97% over 34bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA7466900.3112536245046678RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA7401410.3085237098320714RNA PCR Primer, Index 1 (100% over 22bp)
CAGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAG7351350.30643698623289317No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA6787540.2829348761159803RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAAG6740730.2809836269813912No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG5981750.24934596263252445Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTTGGAATTCTCGGGTGCCAAGG5940560.24762898011054785Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA5894650.2457152469815372No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA5868420.2446218638411768RNA PCR Primer, Index 1 (100% over 22bp)
TCCCATGGTGTAATGGTTAGCACTCTGGTGGAATTCTCGGGTGCCAAGGA5767230.2404038142806326RNA PCR Primer, Index 1 (100% over 22bp)
TGGAGGTGATGAACTGTCTGAGCCTGACCTGGAATTCTCGGGTGCCAAGG5742190.2393600356365371Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATTAAAGTCCTACGTGATCTGAGTTCTGGAATTCTCGGGTGCCAAG5666490.23620452272287767No Hit
TAGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAA5334400.2223615335089127No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAA5163670.2152447472506687RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAA5153100.21480414260737438No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGGAATTCTCGGGTG5142880.21437812752180507No Hit
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACT5056400.21077325623021634RNA PCR Primer, Index 37 (96% over 33bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA4899210.20422087743367576No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGGA4817330.2008077546150439RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA4646630.19369221889031923RNA PCR Primer, Index 1 (100% over 23bp)
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA4504260.18775760365230487RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAAC4498040.1874983263693067RNA PCR Primer, Index 1 (100% over 24bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAGG4492380.18726239237866849Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA4425900.18449121009993563No Hit
GACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAAC4324220.18025273515857648RNA PCR Primer, Index 1 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG4316980.17995093973592266Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA4293740.1789821931029842No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC4251100.1772047681275755RNA PCR Primer, Index 1 (100% over 24bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA4221960.17599008323584453No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC4186290.1745031988814161RNA PCR Primer, Index 1 (100% over 24bp)
CGCAGCAACCTCGGTTCGTATCCGAGTCACGTGGAATTCTCGGGTGCCAA4092880.17060945434687286No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA3949710.16464149154834423No Hit
CGGCGGCGGCGACTCTGGACGCGAGCCGGGTGGAATTCTCGGGTGCCAAG3523060.1468568206815968No Hit
TCGTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGG3330150.13881547330809568Illumina Small RNA Adapter 2 (100% over 21bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCTGGAATTCTCGGGTGCCAAGGA3273270.13644446175553362RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA3219230.13419183404279714RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACCTGGAATTCTCGGGTGCCAAGG3198900.13334438916122915Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA3169380.1321138641782539No Hit
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG3099210.12918886627664916No Hit
CCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG3048490.12707463094004673No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA2890660.12049557409509477No Hit
CAGTCTGATTATTTAGCAAGACTGAGGCTTTGGAATTCTCGGGTGCCAAG2884690.1202467179247573No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT2869090.11959644049472974No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTGGAATTCTCGGGTGCCAAGGA2822290.11764560820464705RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2643340.11018617576211932No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA2608400.10872972105666014No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAA2607730.10870179247472946No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG2607290.10868345131644665No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGG2580820.10758006390793193Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATGGTGTAATGGTTAGCACTCTGGTGGAATTCTCGGGTGCCAAGGAA2569790.10712028441734192RNA PCR Primer, Index 1 (100% over 23bp)
TAGGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAG2531330.10551710044562011No Hit
CAACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAG2526760.10532660250618256No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGGA2508390.10456085914787447RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGG2477640.10327906229060858Illumina Small RNA Adapter 2 (100% over 21bp)
GCTGTGATGGCCGAGTGGTTAAGGCTTTGGTGGAATTCTCGGGTGCCAAG2473930.10312441297872382No Hit
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACTC2425620.10111063716817051RNA PCR Primer, Index 37 (96% over 32bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAG2419790.10086761682092305No Hit
CCCTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGAACT2408230.10038574457149234RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)