FastQCFastQC Report
Thu 13 Jul 2023
SRR3306587.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306587.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242516177
Total Bases12.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT165639146.830024374002893RNA PCR Primer, Index 37 (96% over 33bp)
CATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG111667404.60453407196832RNA PCR Primer, Index 1 (100% over 29bp)
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTC62243612.566575589718289RNA PCR Primer, Index 37 (96% over 32bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC48076561.9824063118065727RNA PCR Primer, Index 37 (97% over 34bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA43261361.783854608593801No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA42572511.755450317856528RNA PCR Primer, Index 1 (100% over 23bp)
AGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAA32567581.3429034055736413RNA PCR Primer, Index 37 (97% over 35bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA30585481.2611727752907798RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA28423141.1720100634771262RNA PCR Primer, Index 1 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG27592291.1377504932382305No Hit
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC26858241.1074824093074829RNA PCR Primer, Index 1 (100% over 24bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA24483281.0095524472992166RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG23033100.9497551992170816Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG21781710.8981549300935912Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG18709470.7714730716705962No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA18443750.760516276817278RNA PCR Primer, Index 1 (100% over 23bp)
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG13443410.5543304436965457Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG13294180.5481770397526925No Hit
GGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCC12776300.5268225880040984RNA PCR Primer, Index 37 (96% over 31bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG11811740.48704957113025904No Hit
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA11268360.46464364313313417RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTTGGAATTCTCGGGTGCCAA10956560.4517867688471767No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAAG10872910.4483375144083687No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG10832410.44666752271952564Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACT10071450.415289822088858RNA PCR Primer, Index 37 (96% over 33bp)
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGG10024610.41335840454057626Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA9791650.40375244740890004RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA8423990.34735785893573606RNA PCR Primer, Index 1 (100% over 23bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTTGGAATTCTCGGGTGCCAAGG7829220.3228328970401014Illumina Small RNA Adapter 2 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAAC7257770.2992695204823388RNA PCR Primer, Index 1 (100% over 24bp)
ATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT7181490.2961241632965375RNA PCR Primer, Index 1 (100% over 30bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG6995360.2884492113695162No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA6827330.2815206014071383RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAAC6408710.26425907249890385RNA PCR Primer, Index 1 (100% over 24bp)
TCTTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGG6334000.2611784532625219Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGGA6152140.2536795720641762RNA PCR Primer, Index 1 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGGA6044450.249239043546361RNA PCR Primer, Index 1 (100% over 22bp)
CCCATGGTGTAATGGTTAGCACTCTGGTGGAATTCTCGGGTGCCAAGGAA5870540.24206797553138074RNA PCR Primer, Index 1 (100% over 23bp)
TAGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAA5836170.24065075048581192No Hit
CAGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAG5554710.22904492676379273No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG5231020.21569777590548112Illumina Small RNA Adapter 2 (100% over 21bp)
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAACTC5144600.2121343022820288RNA PCR Primer, Index 37 (96% over 32bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGGA5102130.21038307889869137RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA4923480.2030165600045724RNA PCR Primer, Index 1 (100% over 22bp)
GCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA4738180.19537583259858168RNA PCR Primer, Index 37 (96% over 30bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGGGCCAAGGAAC4617050.19038111424624676RNA PCR Primer, Index 37 (97% over 34bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG4499430.18553112850694492Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG4200100.17318844672370043Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA4154500.17130815978515115RNA PCR Primer, Index 1 (100% over 23bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTGGAATTCTCGGGTGCCAAGGA4005660.1651708372427461RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAA4001530.16500053932484676No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGA3836920.15821295088286008RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACCTGGAATTCTCGGGTGCCAAGG3777190.15575002239953667Illumina Small RNA Adapter 2 (100% over 21bp)
TCTTGGTCTAGGGGTATGATTCTCGCTTTGGAATTCTCGGGTGCCAAGGA3688270.1520834628693656RNA PCR Primer, Index 1 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA3566640.14706812733568697RNA PCR Primer, Index 1 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAGG3516920.14501795482286528Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA3420210.14103017960735872RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA3381010.13941379259000938No Hit
TCCCACATGGTCTAGCGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA3375460.13918494187709385RNA PCR Primer, Index 1 (100% over 23bp)
CCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG3267450.13473121836321872No Hit
CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTG3255030.13421908757864015RNA PCR Primer, Index 37 (97% over 43bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAA3113440.12838071416571933No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTCTGGAATTCTCGGGTGCCAAG3032920.12506052328212316No Hit
GGTCGCTGGTTCGTATCCGGCTCGGAGGTGGAATTCTCGGGTGCCAAGGA3015360.12433644787333094RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG2887280.11905515070031802No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCA2825520.11650851646073904No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG2802550.11556136314980751Illumina Small RNA Adapter 2 (100% over 21bp)
CCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAAG2466880.10172022462649986No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGA2448790.10097429500548329RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)