FastQCFastQC Report
Mon 10 Jul 2023
SRR3306588.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306588.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173946221
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2176516212.512581115516157No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG123285877.087585421013544Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG40650692.336968849699816No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG36827902.1172003501013106Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG28175191.61976442132652Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA27882111.6029155356010867No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA23483771.3500592231894477RNA PCR Primer, Index 1 (100% over 22bp)
ACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAG15707440.9030055329572236No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA13707140.7880102206991896RNA PCR Primer, Index 1 (100% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG11323990.651005232243591No Hit
CTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGG10825690.6223584472122565Illumina Small RNA Adapter 2 (100% over 21bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG8420790.4841030722938212Illumina Small RNA Adapter 2 (100% over 21bp)
TGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGA6903760.3968904849045269RNA PCR Primer, Index 1 (100% over 22bp)
ACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGA6893960.3963270923833407RNA PCR Primer, Index 1 (100% over 22bp)
TTGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG6861450.3944581239278547Illumina Small RNA Adapter 2 (100% over 21bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA6724390.38657867709583643No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA6211350.35708450372141165RNA PCR Primer, Index 1 (100% over 22bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGG5767940.3315932916990476Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATTCGAACGTCTGCCCTATCAACTTTGGAATTCTCGGGTGCCAAGGA5617940.32296993678293245RNA PCR Primer, Index 1 (100% over 22bp)
GTGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGGA5408240.3109144866102035RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG5239790.3012304590394062No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTGGAATTCTCGGGTGCCAAGG4892220.281248995918112Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA4800620.2759830005160043RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG4462460.25654250919311433Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4430620.254712058389587No Hit
CCTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTC4374970.2515127937157083RNA PCR Primer, Index 1 (100% over 26bp)
GTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAA4340610.24953747054959016RNA PCR Primer, Index 1 (100% over 23bp)
GTGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG4163480.23935443817431368No Hit
TACTTGGATAACTGTGGTAATTCTAGAGTGGAATTCTCGGGTGCCAAGGA3975070.22852292950934527RNA PCR Primer, Index 1 (100% over 22bp)
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA3849760.22131897881242274RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAATCAGTTATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAG3535290.2032404026759512No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG3429500.19715863789877908No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTGGAATTCTCGGGTGCCAAGGA3379730.1942974087376121RNA PCR Primer, Index 1 (100% over 22bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA3368600.1936575558028363RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGAATTCTCGGGTGCCAAGGCTGGAATTCTCGGGTGCCAAGGAACTC3337640.19187769534815016RNA PCR Primer, Index 1 (100% over 26bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG3182820.18297724329406384Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGGATACCGCAGCTAGGAATAATGGATGGAATTCTCGGGTGCCAAG2897840.1665940187341006No Hit
TAAATCAGTTATGGTTCCTTTGGTCGCTTGGAATTCTCGGGTGCCAAGGA2831950.1628060663646151RNA PCR Primer, Index 1 (100% over 22bp)
GACCCATTCGAACGTCTGCCCTATCAACTTTGGAATTCTCGGGTGCCAAG2630610.15123122450587762No Hit
CCCGGGGAGGTAGTGACGAAAAATAACAATGGAATTCTCGGGTGCCAAGG2568670.14767035381584978Illumina Small RNA Adapter 2 (100% over 21bp)
GATGTTGGATCAGGACATCCCGATGGTGCTGGAATTCTCGGGTGCCAAGG2529060.14539321322766763Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2368210.13614610230595353No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAAGG2304130.13246220508578913Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCATGGAATTCTCGGGTGCCAAG2204140.12671387669870676No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA2109890.12129553535974777No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAAG2083560.11978184912680569No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG1994490.11466130097761652No Hit
CCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTA1917330.11022544720876691RNA PCR Primer, Index 2 (100% over 47bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCTGGAATTCTCGGGTGCCA1812990.10422704152911721No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAA1804030.10371193979546126No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGGGCCAAG1798840.10341357171536368No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG1783560.10253513929457542Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG1781450.10241383743542208No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)