FastQCFastQC Report
Thu 13 Jul 2023
SRR3306589.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3306589.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242417925
Total Bases12.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT212475168.764828755959362RNA PCR Primer, Index 37 (96% over 33bp)
CATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAG156734416.465462898422218RNA PCR Primer, Index 1 (100% over 29bp)
CGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTC72552182.9928554169416306RNA PCR Primer, Index 37 (96% over 32bp)
GACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC58412992.4095986301343024RNA PCR Primer, Index 37 (97% over 34bp)
CGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAAC49437382.039345069057909RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA49173122.0284440599844253No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG44551021.8377774663321615No Hit
AGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAA36471391.5044840434138689RNA PCR Primer, Index 37 (97% over 35bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAAG36441991.5032712618095383No Hit
AGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAAG31622051.3044435554837788No Hit
TCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAA25509761.0523050224111934RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAGG22775070.9394961201817068Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA18732960.7727547375054876RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAGGA14661450.6048005732249379RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG12432380.5128490395254394Illumina Small RNA Adapter 2 (100% over 21bp)
GGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCC11249540.4640556179993703RNA PCR Primer, Index 37 (96% over 31bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGGAATTCTCGGGTGCCAAGG10289120.42443726056973713Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG9794930.40405139182673894No Hit
CGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAA9359570.38609232382465114RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGAA9322840.3845771718407374RNA PCR Primer, Index 1 (100% over 23bp)
ATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGT9232170.3808369368725518RNA PCR Primer, Index 1 (100% over 30bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGTGGAATTCTCGGGTGCCAAGGA9051900.3734006055864062RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACCTGGAATTCTCGGGTGCCAAGG8870440.3659151855210377Illumina Small RNA Adapter 2 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGTGGAATTCTCGGGTGCCAAG8564420.35329153155650517No Hit
GAATACAAGCTTGGGCTGCAGGTCGACTGGAATTCTCGGGTGCCAAGGAA8060880.3325199652624698RNA PCR Primer, Index 1 (100% over 23bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGTGGAATTCTCGGGTGCCAAG6931340.28592522603268716No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTGGAATTCTCGGGTGCCA6378200.26310760641978104No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGGAATTCTCGGGTGCCAAG6133370.25300810573310534No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGGA6010800.24795196147314602RNA PCR Primer, Index 1 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCTGGAATTCTCGGGTGCCAAGGA5989090.24705640063539033RNA PCR Primer, Index 1 (100% over 22bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTTGGAATTCTCGGGTGCCAAGG5867730.24205017017615346Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA5478550.22599607681651426RNA PCR Primer, Index 1 (100% over 22bp)
TAGTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAA5442480.22450815054208553No Hit
AAACCTGCATTAAAAATTTCGGTTGGGTGGAATTCTCGGGTGCCAAGGAA5414120.2233382700557312RNA PCR Primer, Index 1 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGA5343210.2204131563290957RNA PCR Primer, Index 1 (100% over 22bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAGG5316290.21930267739070863Illumina Small RNA Adapter 2 (100% over 21bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG5277730.21771203593958657No Hit
GGTCGCTGGTTCGTATCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGGA4938160.20370440840956788RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGCTGGTTCGTTTCCGGCTCGAAGGTGGAATTCTCGGGTGCCAAGG4871330.20094759906883947Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG4855750.2003049073206942Illumina Small RNA Adapter 2 (100% over 21bp)
GCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA4392010.18117513381075265RNA PCR Primer, Index 37 (96% over 30bp)
CGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4315370.1780136514244976RNA PCR Primer, Index 1 (100% over 33bp)
CCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACTCCA4188170.17276651468739368RNA PCR Primer, Index 1 (100% over 28bp)
CCGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAACT3952880.16306054925600078RNA PCR Primer, Index 37 (96% over 29bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAA3726040.15370315540816545No Hit
AGTTCAACTTTAAATTTGCCCACAGAATGGAATTCTCGGGTGCCAAGGAA3605830.14874436368515243RNA PCR Primer, Index 1 (100% over 23bp)
AGTGCGGTAACGCGACCGATCCCGGAGATGGAATTCTCGGGTGCCAAGGA3544270.146204947509554RNA PCR Primer, Index 1 (100% over 22bp)
GGTCGCTGGTTCGTATCCGGCTCGGAGGTGGAATTCTCGGGTGCCAAGGA3482690.14366470631245606RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACTGGAATTCTCGGGTGCCAAGGA3416840.14094832302520532RNA PCR Primer, Index 1 (100% over 22bp)
CACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTGCCAAGGAAC3276720.13516822239939558RNA PCR Primer, Index 37 (96% over 30bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTGGAATTCTCGGGTGCCAAGGA3228060.13316094509100349RNA PCR Primer, Index 1 (100% over 22bp)
GTTCAACTTTAAATTTGCCCACAGAACCCTGGAATTCTCGGGTGCCAAGG3140020.129529200450008Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG3077120.12693450783394009No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCTGGAATTCTCGGGTGCCAAGG3051860.1258925056800152Illumina Small RNA Adapter 2 (100% over 21bp)
CAAGCAGAAGACGGCATACGAGATATTCCGGTGACTGGAATTCTCGGGTG3010820.1241995615629496RNA PCR Primer, Index 37 (97% over 43bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG3003490.12389719118336649Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATTAAAGTCCTACGTGATCTGAGTTGGAATTCTCGGGTGCCAAGGAA2972280.12260974513332709RNA PCR Primer, Index 1 (100% over 23bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACTGGAATTCTCGGGTGCCAAGGA2604690.10744626248244639RNA PCR Primer, Index 1 (100% over 22bp)
AGGTCGGTTTCTATCTACTTCAAATTCCTGGAATTCTCGGGTGCCAAGGA2575880.10625781901235233RNA PCR Primer, Index 1 (100% over 22bp)
AGTTCAACTTTAAATTTGCCCACAGAACCCTTGGAATTCTCGGGTGCCAA2471640.10195780695672566No Hit
GACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGGAAC2467580.1017903275923181RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)