FastQCFastQC Report
Thu 29 Jun 2023
SRR3312945.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3312945.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36979757
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT4316891.1673657022678652RNA PCR Primer, Index 1 (100% over 25bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG2771910.7495749634049786Illumina Small RNA Adapter 2 (100% over 21bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCAAGG1550640.4193213059782951Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT1537800.4158491360557075RNA PCR Primer, Index 1 (100% over 25bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGGGTGCCAAGGA1317750.35634360712537944RNA PCR Primer, Index 1 (100% over 22bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGA1275220.3448427203023535RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC1155290.3124114633852245RNA PCR Primer, Index 1 (100% over 26bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC1140940.3085309619530491RNA PCR Primer, Index 1 (100% over 24bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA1001560.27084007069056726RNA PCR Primer, Index 1 (100% over 22bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG892040.24122386742562962Illumina Small RNA Adapter 2 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC817050.22094520523755742RNA PCR Primer, Index 1 (100% over 24bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA677250.18314073832340216RNA PCR Primer, Index 1 (100% over 22bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC648400.17533917272631078RNA PCR Primer, Index 1 (100% over 24bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA629810.17031209804867026RNA PCR Primer, Index 1 (100% over 23bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG536990.14521187902884272No Hit
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAAGGA533570.14428704872235912RNA PCR Primer, Index 1 (100% over 22bp)
AGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAACT530660.1435001317071932RNA PCR Primer, Index 1 (100% over 25bp)
GCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAGGA518980.14034164691779882RNA PCR Primer, Index 1 (100% over 22bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC497900.13464123087666585RNA PCR Primer, Index 1 (100% over 27bp)
AAGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAA493210.13337296943297924RNA PCR Primer, Index 1 (100% over 23bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC489460.1323589011144665RNA PCR Primer, Index 1 (100% over 26bp)
ATGTCCTAAGGGGGGCTCATGGAGAACATGGAATTCTCGGGTGCCAAGGA482940.13059577433134564RNA PCR Primer, Index 1 (100% over 22bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC434090.11738584436885294RNA PCR Primer, Index 1 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA424760.11486284239239322RNA PCR Primer, Index 1 (100% over 23bp)
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC424180.11470599982579659RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT414280.11202885946492293RNA PCR Primer, Index 1 (100% over 25bp)
ACGATCAACTTAGAACTGGTACGGACAAGTGGAATTCTCGGGTGCCAAGG409130.1106362056408321Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATATGGAATTCTCGGGTGCCAAG407410.11017108630540758No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGAA407250.11012781939048435RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATATGGAATTCTCGGGTGCCAAGG407240.11012511520830166Illumina Small RNA Adapter 2 (100% over 21bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA399050.10791039000066982No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTGCCAAGGAACT396960.1073452159244854RNA PCR Primer, Index 1 (100% over 25bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA392110.10603368756587556RNA PCR Primer, Index 1 (100% over 22bp)
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGTGCCAAGGAAC380950.10301582024998164RNA PCR Primer, Index 1 (100% over 24bp)
GGGAATACTGCCAGCTGGGACTGAGGATGGAATTCTCGGGTGCCAAGGAA375800.1016231664258908RNA PCR Primer, Index 1 (100% over 23bp)
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGGTGCCAAGGAA373950.10112289272209117RNA PCR Primer, Index 1 (100% over 23bp)
ATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACT370530.10019806241560755RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)