FastQCFastQC Report
Thu 29 Jun 2023
SRR3312946.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3312946.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31149275
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACT6644462.133102616353029RNA PCR Primer, Index 1 (100% over 25bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGG3797881.2192514914071033Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGAAC2188920.7027194051867981RNA PCR Primer, Index 1 (100% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGATCTATCTCGTATGC1896980.6089965175754491RNA PCR Primer, Index 2 (97% over 37bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGA1721180.5525586069017657RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGAACT1564070.5021208358782026RNA PCR Primer, Index 1 (100% over 25bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTTGGAATTCTCGGGTGCCAAGGA1386970.4452655800175125RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATGGAATTCTCGGGTGCCAAGGAACTC1366730.43876783649057643RNA PCR Primer, Index 1 (100% over 26bp)
CCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCAAGG1223390.3927507141016926Illumina Small RNA Adapter 2 (100% over 21bp)
TTCGGTGCCCGAGTTGTAATTTGTGGAATTCTCGGGTGCCAAGGAACTCC947970.3043313207129219RNA PCR Primer, Index 1 (100% over 27bp)
CTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGAA913550.2932813043000198RNA PCR Primer, Index 1 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATATGGAATTCTCGGGTGCCAAGGAAC906440.2909987471618521RNA PCR Primer, Index 1 (100% over 24bp)
ACGCGAAACTCAGGTGCTGCAATCTTGGAATTCTCGGGTGCCAAGGAACT800460.25697548337802406RNA PCR Primer, Index 1 (100% over 25bp)
AGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAAC778010.2497682530331765RNA PCR Primer, Index 1 (100% over 24bp)
AATGGCTTAGTGAGGCCTCAGGATCTGGAATTCTCGGGTGCCAAGGAACT719620.23102303344138828RNA PCR Primer, Index 1 (100% over 25bp)
AATGGCTTAGTGAGGCCTCAGGATCTTGGAATTCTCGGGTGCCAAGGAAC708930.2275911718651558RNA PCR Primer, Index 1 (100% over 24bp)
GGCGCCAGTGAAATACCACTACCTTTATGGAATTCTCGGGTGCCAAGGAA666940.21411092232483742RNA PCR Primer, Index 1 (100% over 23bp)
GAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGAACTC652600.20950728387739362RNA PCR Primer, Index 1 (100% over 26bp)
AGGGAGGTAGTGACAATAAATAACGTGGAATTCTCGGGTGCCAAGGAACT640720.20569339093767028RNA PCR Primer, Index 1 (100% over 25bp)
AAGCCATGCATGTCTAAGTATAAGCAATGGAATTCTCGGGTGCCAAGGAA623830.2002711138541748RNA PCR Primer, Index 1 (100% over 23bp)
CTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAAC604290.19399809465870393RNA PCR Primer, Index 1 (100% over 24bp)
ATGTCCTAAGGGGGGCTCATGGAGAACATGGAATTCTCGGGTGCCAAGGA600110.19265616936509758RNA PCR Primer, Index 1 (100% over 22bp)
ATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAACT591990.1900493671201015RNA PCR Primer, Index 1 (100% over 25bp)
ACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAGGA570740.18322737848633716RNA PCR Primer, Index 1 (100% over 22bp)
ATGGCTTAGTGAGGCCTCAGGATCTGCTGGAATTCTCGGGTGCCAAGGAA528120.16954487704770013RNA PCR Primer, Index 1 (100% over 23bp)
TGCAAGAGTTCGAATCTCTTAGCAACCACCATGGAATTCTCGGGTGCCAA499680.16041464849502918No Hit
ATAAATCATTTGTATACGACTTAGATTGGAATTCTCGGGTGCCAAGGAAC484750.15562159953963617RNA PCR Primer, Index 1 (100% over 24bp)
AATGGCTTAGTGAGGCCTCAGGATCTGTGGAATTCTCGGGTGCCAAGGAA448960.1441317655065808RNA PCR Primer, Index 1 (100% over 23bp)
GGCGCCAGTGAAATACCACTACCTTTATTGGAATTCTCGGGTGCCAAGGA416710.13377839452122078RNA PCR Primer, Index 1 (100% over 22bp)
ACTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGGAA403050.12939305971005746RNA PCR Primer, Index 1 (100% over 23bp)
ATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCAAGGAACT398380.12789382738442548RNA PCR Primer, Index 1 (100% over 25bp)
TGTGGCGTCGCTGAACCATAGCAGTGGAATTCTCGGGTGCCAAGGAACTC397490.12760810644870546RNA PCR Primer, Index 1 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATGGAATTCTCGGGTGCCAAGGAA396550.12730633377502365RNA PCR Primer, Index 1 (100% over 23bp)
AATGACCCACTCGGCACCTTACGAGAAATGGAATTCTCGGGTGCCAAGGA394620.12668673668969824RNA PCR Primer, Index 1 (100% over 22bp)
AATGACCCACTCGGCACCTTACGAGATGGAATTCTCGGGTGCCAAGGAAC389370.1250013042037094RNA PCR Primer, Index 1 (100% over 24bp)
TCGAAGAGTCGAGTTGTTTGGGAATGGAATTCTCGGGTGCCAAGGAACTC387080.12426613460505903RNA PCR Primer, Index 1 (100% over 26bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTGGAATTCTCGGGTGCCAAGGA375610.12058386591662246RNA PCR Primer, Index 1 (100% over 22bp)
TTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAAGGA319700.10263481252773943RNA PCR Primer, Index 1 (100% over 22bp)
AATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAAC318750.10232982950646524RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)